LOCUS       3196bp_PCR_prod         3196 bp    DNA     linear   UNK 03-MAR-2015
DEFINITION  Product_775_578
ACCESSION   3196bp CnmV4IyrtwWBeFKbnEjd/ZW/kyY
VERSION     3196bp CnmV4IyrtwWBeFKbnEjd/ZW/kyY
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     1..18
                     /note="775"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            1..18
                     /label="775"
     overlap         13..724
                     /note="olp_t+5ztakvUkuGtUKBpYQWSwQAfDU"
                     /ApEinfo_revcolor="#D1F5C6"
                     /chksum="t+5ztakvUkuGtUKBpYQWSwQAfDU"
                     /ApEinfo_fwdcolor="#F9F2C5"
     promoter        20..717
                     /note="tp YGR193C YGR192C"
                     /ApEinfo_revcolor="#b1e681"
                     /ApEinfo_fwdcolor="#b1e6cc"
     primer_bind     20..41
                     /note="415_ScTDH3tpf"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(692..717)
                     /note="623_ScTDH3tpr_PacI"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     CDS             765..1856
                     /product="xylitol dehydrogenase"
                     /codon_start=1
                     /transl_table=12
                     /db_xref="GI:640922"
                     /translation="MTANPSLVLNKIDDISFETYDAPEISEPTDVLVQVKKTGICGSDI
                     HFYAHGRIGNFVLTKPMVLGHESAGTVVQVGKGVTSLKVGDNVAIEPGIPSRFSDEYKS
                     GHYNLCPHMAFAATPNSKEGEPNPPGTLCKYFKSPEDFLVKLPDHVSLELGALVEPLSV
                     GVHASKLGSVAFGDYVAVFGAGPVGLLAAAVAKTFGAKGVIVVDIFDNKLKMAKDIGAA
                     THTFNSKTGGSEELIKAFGGNVPNVVLECTGAEPCIKLGVDAIAPGGRFVQVGNAAGPV
                     SFPITVFAMKELTLFGSFRYGFNDYKTAVGIFDTNYQNGRENAPIDFEQLITHRYKFKD
                     AIEAYDLVRAGKGAVKCLIDGPE"
                     /protein_id="CAA01269.1"
     primer_bind     complement(1833..1856)
                     /note="XDH_YPK_rec_rev"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     primer_bind     1895..1915
                     /note="549_ScPGI1tpf"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     gene            2165..2269
                     /locus_tag="YBR196C-B"
     mRNA            2165..2269
                     /locus_tag="YBR196C-B"
                     /product="hypothetical protein"
     CDS             2165..2269
                     /product="hypothetical protein"
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     ND]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     ND]"
                     /codon_start=1
                     /locus_tag="YBR196C-B"
                     /note="hypothetical protein; identified by expression
                     profiling and mass spectrometry"
                     /db_xref="SGD:S000028816"
                     /GO_process="GO:0008150 - biological_process [Evidence ND]"
                     /translation="MWVVLSKEKILLKKAYYAKTILFSALVLRGVRGE"
                     /protein_id="DAA07312.1"
     gene            2622..2771
                     /locus_tag="YBR196C-A"
     mRNA            2622..2771
                     /locus_tag="YBR196C-A"
                     /product="hypothetical protein"
     CDS             2622..2771
                     /product="hypothetical protein"
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     ND]"
                     /GO_component="GO:0016021 - integral to membrane [Evidence
                     IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA,IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     ND]"
                     /codon_start=1
                     /locus_tag="YBR196C-A"
                     /note="hypothetical protein; identified by fungal homology
                     and RT-PCR"
                     /db_xref="SGD:S000028534"
                     /GO_process="GO:0008150 - biological_process [Evidence ND]"
                     /translation="MSRVYIYPLTVFYFFAIEMSVFCYYNWFYRRNFPYLFRPIFPFLI
                     VLIS"
                     /protein_id="DAA07311.1"
     primer_bind     complement(2874..2894)
                     /note="622_ScPGI1tpr_PacI"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(2939..2962)
                     /note="166_pCAPSrv"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     overlap         2955..3196
                     /note="olp_WozPxSoJjWK29v8yQeT4MATRraA"
                     /ApEinfo_revcolor="#F7BCF3"
                     /chksum="WozPxSoJjWK29v8yQeT4MATRraA"
                     /ApEinfo_fwdcolor="#FEF5E0"
     primer_bind     complement(3168..3196)
                     /note="578"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(3168..3196)
                     /label="578"
ORIGIN
        1 gcggccgctg acttaaataa taaaaaacac gctttttcag ttcgagttta tcattatcaa
       61 tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta actttattta
      121 gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat agggggcggg
      181 ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct ggcatccact
      241 aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat cccagcacca
      301 aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc gcaactacag
      361 agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga tgcaacctgc
      421 ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc attttcttac
      481 accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc
      541 agttccctga aattattccc ctacttgact aataagtata taaagacggt aggtattgat
      601 tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag ttagtctttt
      661 ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac aaacaaatta
      721 attagtcgag gaacgccagg ttgcccactt tctcactagt gaaaatgact gctaaccctt
      781 ccttggtgtt gaacaagatc gacgacattt cgttcgaaac ttacgatgcc ccagaaatct
      841 ctgaacctac cgatgtcctc gtccaggtca agaaaaccgg tatctgtggt tccgacatcc
      901 acttctacgc ccatggtaga atcggtaact tcgttttgac caagccaatg gtcttgggtc
      961 acgaatccgc cggtactgtt gtccaggttg gtaagggtgt cacctctctt aaggttggtg
     1021 acaacgtcgc tatcgaacca ggtattccat ccagattctc cgacgaatac aagagcggtc
     1081 actacaactt gtgtcctcac atggccttcg ccgctactcc taactccaag gaaggcgaac
     1141 caaacccacc aggtacctta tgtaagtact tcaagtcgcc agaagacttc ttggtcaagt
     1201 tgccagacca cgtcagcttg gaactcggtg ctcttgttga gccattgtct gttggtgtcc
     1261 acgcctccaa gttgggttcc gttgctttcg gcgactacgt tgccgtcttt ggtgctggtc
     1321 ctgttggtct tttggctgct gctgtcgcca agaccttcgg tgctaagggt gtcatcgtcg
     1381 ttgacatttt cgacaacaag ttgaagatgg ccaaggacat tggtgctgct actcacacct
     1441 tcaactccaa gaccggtggt tctgaagaat tgatcaaggc tttcggtggt aacgtgccaa
     1501 acgtcgtttt ggaatgtact ggtgctgaac cttgtatcaa gttgggtgtt gacgccattg
     1561 ccccaggtgg tcgtttcgtt caagttggta acgctgctgg tccagtcagc ttcccaatca
     1621 ccgttttcgc catgaaggaa ttgactttgt tcggttcttt cagatacgga ttcaacgact
     1681 acaagactgc tgttggaatc tttgacacta actaccaaaa cggtagagaa aatgctccaa
     1741 ttgactttga acaattgatc acccacagat acaagttcaa ggacgctatt gaagcctacg
     1801 acttggtcag agccggtaag ggtgctgtca agtgtctcat tgacggccct gagtaacgcg
     1861 ccatctgtgc agacaaacgc atcaggattt aaataattca gttttctgac tgagttaaaa
     1921 actaatgtag cgacaccact tccattggtg cctccgttat tttttttttg tatgttaatg
     1981 ctaaataata caccgctatg tatttcaggg cactacttct acacatcaac ggtactaaac
     2041 atttcgctca agatcggtcc gctttcacgt atttggatgc tatgcaatgt tgactattct
     2101 tagtggataa catgcggcat ttcctggcgg ctatttgcat cgctatgtaa gtggcaatgt
     2161 tcggatgtgg gtagtacttt caaaagaaaa aatattactg aagaaggcat actacgccaa
     2221 gactatttta ttctcagcac ttgtcctacg tggggttaga ggcgagtaag actttggccc
     2281 cgctgcggaa gcaaagagaa ttttgccatc ggacatgcta ccttacgctt atatctctca
     2341 ttggaatatc gttttctgat taaaacacgg aagtaagaac ttaattcgtt tttcgttgaa
     2401 ctatgttgtg ccagcgtaac attaaaaaag agtgtacaag gccacgttct gtcaccgtca
     2461 gaaaaatatg tcaatgaggc aagaaccggg atggtaacaa aaatcacgat ctgggtgggt
     2521 gtgggtgtat tggattatag gaagccacgc gctcaacctg gaattacagg aagctggtaa
     2581 ttttttgggt ttgcaatcat caccatctgc acgttgttat aatgtcccgt gtctatatat
     2641 atccattgac ggtattctat ttttttgcta ttgaaatgag cgttttttgt tactacaatt
     2701 ggttttacag acggaatttt ccctatttgt ttcgtcccat ttttcctttt ctcattgttc
     2761 tcatatctta aaaaggtcct ttcttcataa tcaatgcttt cttttactta atattttact
     2821 tgcattcagt gaattttaat acatattcct ctagtcttgc aaaatcgatt tagaatcaag
     2881 ataccagcct aaaattaatt aatccggatt tacctgaatc aattggcgaa attttttgta
     2941 cgaaatttca gccacttcac aggcggtttt cgcacgtacc catgcgctac gttcctggcc
     3001 ctcttcaaac aggcccagtt cgccaataaa atcaccctga ttcagatagg agaggatcat
     3061 ttctttaccc tcttcgtctt tgatcagcac tgccacagag cctttaacga tgtagtacag
     3121 cgtttccgct ttttcaccct ggtgaataag cgtgctcttg gatgggtact tatgaatgtg
     3181 gcaatgagac aagaac
//