LOCUS       pYPK0_tp_gene_tp        8746 bp    DNA     circular UNK 28-MAR-2014
DEFINITION  pYPK0_RPL12Btp_CiGXF1_TDH3tp
ACCESSION   -
VERSION     -
KEYWORDS    .
SOURCE      
  ORGANISM  . .
            .
FEATURES             Location/Qualifiers
     overlap         433..530
                     /note="olp_pX1elTSrsO+Y7S81pRpDvK/GuRY"
                     /ApEinfo_revcolor="#EEDFB1"
                     /chksum="pX1elTSrsO+Y7S81pRpDvK/GuRY"
                     /ApEinfo_fwdcolor="#C3FAB4"
     primer_bind     537..558
                     /note="pfw604"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(1116..1140)
                     /note="prv604"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     overlap         1148..1197
                     /note="olp_8ro7GZnjXs9RDyEisndJYyFeLIU"
                     /ApEinfo_revcolor="#D5EEEF"
                     /chksum="8ro7GZnjXs9RDyEisndJYyFeLIU"
                     /ApEinfo_fwdcolor="#D9F5BE"
     primer_bind     complement(1175..1197)
                     /note="567_pCAPsAjiIF"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     overlap         2853..2889
                     /note="olp_+ALddA7RDRqA4MsR6S2vDiSMYD0"
                     /ApEinfo_revcolor="#DFB6CB"
                     /chksum="+ALddA7RDRqA4MsR6S2vDiSMYD0"
                     /ApEinfo_fwdcolor="#C3E0F2"
     promoter        2891..3588
                     /note="tp YGR193C YGR192C"
                     /ApEinfo_fwdcolor="#b1e6cc"
                     /ApEinfo_revcolor="#b1e681"
     primer_bind     2891..2912
                     /note="415_ScTDH3tpf"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     primer_bind     complement(3563..3588)
                     /note="623_ScTDH3tpr_PacI"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     overlap         3596..3656
                     /note="olp_OmDbOULBfMl1s6bOD56K7WGX+Bw"
                     /ApEinfo_revcolor="#F7D5D1"
                     /chksum="OmDbOULBfMl1s6bOD56K7WGX+Bw"
                     /ApEinfo_fwdcolor="#B5B0F0"
     primer_bind     complement(3633..3656)
                     /note="166_pCAPSrv"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     rep_origin      complement(4279..4961)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="gray50"
                     /label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
     misc_feature    5041..6071
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="green"
                     /label="URA3 marker"
                     /ApEinfo_fwdcolor="cyan"
     misc_feature    6074..7545
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="green"
                     /label="2micron 2µ"
                     /ApEinfo_fwdcolor="cyan"
     CDS             complement(7693..8352)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="yellow"
                     /label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
      241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
      301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
      361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
      421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
      481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac ttaaattata
      541 agctatgtaa aatcatgcat cattttcgaa accataagtt cttttttggt gcggccttct
      601 gcaattactg attaagtagc aattttcctt caaacatccg tgcactgtgg atgtttgggt
      661 tgtgtatttt gctttcataa catacagata ttttgtttaa ggaagtgaaa taaacaatat
      721 cataaaacag gtacttcata gaccataaag cataacccag attatcctct tagatagcaa
      781 tgctaatgta aacagagatc cgtttgcgtg actttatact aatatgatat gtctacttcg
      841 ctttgtgttc gagcagcctg gcagtcccct ctagccgctt ttttcccttt ccgaaggttt
      901 ccgcctaagc cccctggctc taggccgaga aaatgttaat gctccttcta cgagaaaatg
      961 cttgtcgcca caccaggaca ggtgctcgac gacgcttccg ctaatctttc tcaatgttgt
     1021 atcttctttg gcggtacatt actagtatga aaatggaata aaaacagtac ctaaattatt
     1081 tacttacttc ccgttaaagc aaccccaagt gcccaataga aggataaatc aatagtcaat
     1141 ttaattagtc gaggaacgcc aggttgccca ctttctcact agtgacctgc agccgaccgc
     1201 gccaaaatgt cacaagattc gcattcttct ggtgccgcta caccagtcaa tggttccatc
     1261 cttgaaaagg aaaaagaaga ctctccagtt cttcaagttg atgccccaca aaagggtttc
     1321 aaggactaca ttgtcatttc tatcttctgt tttatggttg ccttcggtgg tttcgtcttc
     1381 ggtttcgaca ctggtaccat ttccggtttc gtgaacatgt ctgactttaa agacagattc
     1441 ggtcaacacc acgctgatgg tactccttac ttgtccgacg ttagagttgg tttgatgatt
     1501 tctattttca acgttggttg cgctgtcggt ggtattttcc tctgcaaggt cgctgatgtc
     1561 tggggtagaa gaattggtct tatgttctcc atggctgtct acgttgttgg tattattatt
     1621 cagatctctt catccaccaa gtggtaccag ttcttcattg gtcgtcttat tgctggtttg
     1681 gctgttggta ccgtttctgt cgtttcccca cttttcatct ctgaggtttc tccaaagcaa
     1741 attagaggta ctttagtgtg ctgcttccag ttgtgtatca ccttgggtat cttcttgggt
     1801 tactgtacta cttacggtac taagacctac actgactcta gacagtggag aattcctttg
     1861 ggtttgtgtt tcgcttgggc tatcttgttg gttgtcggta tgttgaacat gccagagtct
     1921 ccaagatact tggttgagaa gcacagaatt gatgaggcca agagatccat tgccagatcc
     1981 aacaagatcc ctgaggagga cccattcgtc tacactgagg ttcagcttat tcaggccggt
     2041 attgagagag aagctttggc tggtcaggca tcttggaagg agttgatcac tggtaagcca
     2101 aagatcttca gaagagttat catgggtatt atgcttcagt ccttgcaaca gttgaccggt
     2161 gacaactact tcttctacta cggtactacc attttccagg ctgtcggttt gaaggattct
     2221 ttccagactt ctatcatttt gggtattgtc aactttgctt ccaccttcgt tggtatctat
     2281 gtcattgaga gattgggtag aagattgtgt cttttgaccg gttccgctgc tatgttcatc
     2341 tgtttcatca tctactcttt gattggtact cagcacttgt acaagcaagg ttactccaac
     2401 gagacctcca acacttacaa ggcttctggt aacgctatga tcttcatcac ttgtctttac
     2461 attttcttct ttgcttctac ctgggctggt ggtgtttact gtatcatttc cgagtcctac
     2521 ccattgagaa ttagatccaa ggccatgtct attgctaccg ctgctaactg gttgtggggt
     2581 ttcttgattt ccttcttcac tccattcatc accagtgcca tccacttcta ctacggtttc
     2641 gttttcactg gttgtttggc tttctctttc ttctacgtct acttcttcgt ctacgaaacc
     2701 aagggtcttt ctttggagga ggttgatgag atgtacgctt ccggtgttct tccactcaag
     2761 tctgccagct gggttccacc aaatcttgag cacatggctc actctgccgg ttacgctggt
     2821 gctgacaagg ccaccgacga acaggtttaa ccgtgccatc tgtgcagaca aacgcatcag
     2881 gatttaaata ataaaaaaca cgctttttca gttcgagttt atcattatca atactgccat
     2941 ttcaaagaat acgtaaataa ttaatagtag tgattttcct aactttattt agtcaaaaaa
     3001 ttagcctttt aattctgctg taacccgtac atgcccaaaa tagggggcgg gttacacaga
     3061 atatataaca tcgtaggtgt ctgggtgaac agtttattcc tggcatccac taaatataat
     3121 ggagcccgct ttttaagctg gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt
     3181 tttcttcacc aaccatcagt tcataggtcc attctcttag cgcaactaca gagaacaggg
     3241 gcacaaacag gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa
     3301 atgatgacac aaggcaattg acccacgcat gtatctatct cattttctta caccttctat
     3361 taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg
     3421 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     3481 gtaaatctat ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt
     3541 taaaacacca agaacttagt ttcgaataaa cacacataaa caaacaaatt aattaatccg
     3601 gatttacctg aatcaattgg cgaaattttt tgtacgaaat ttcagccact tcacaggcgg
     3661 ttttcgcacg tacccatgcg ctacgttcct ggccctcttc aaacaggccc agttcgccaa
     3721 taaaatcacc ctgattcaga taggagagga tcatttcttt accctcttcg tctttgatca
     3781 gcactgccac agagccttta acgatgtagt acagcgtttc cgctttttca ccctggtgaa
     3841 taagcgtgct cttggatggg tacttatgaa tgtggcaatg agacaagaac cattcgagag
     3901 taggatccgt ttgaggttta ccaagtacca taagatcctt aaatttttat tatctagcta
     3961 gatgataata ttatatcaag aattgtacct gaaagcaaat aaatttttta tctggcttaa
     4021 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     4081 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     4141 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4201 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4261 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4321 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4381 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4441 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4501 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4561 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4621 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4681 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4741 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4921 tcagtggaac gaaaactcac gttaagggat tttggtcatg aggggtaata actgatataa
     4981 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt
     5041 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc
     5101 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc
     5161 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt
     5221 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc
     5281 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac
     5341 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat
     5401 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat
     5461 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac
     5521 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa
     5581 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga
     5641 aaaatcagtc aaaatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
     5701 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
     5761 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
     5821 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg
     5881 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
     5941 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     6001 aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     6061 ttgaaaagct agcttatcga tgataagctg tcaaagatga gaattaattc cacggactat
     6121 agactatact agatactccg tctactgtac gatacacttc cgctcaggtc cttgtccttt
     6181 aacgaggcct taccactctt ttgttactct attgatccag ctcagcaaag gcagtgtgat
     6241 ctaagattct atcttcgcga tgtagtaaaa ctagctagac cgagaaagag actagaaatg
     6301 caaaaggcac ttctacaatg gctgccatca ttattatccg atgtgacgct gcagcttctc
     6361 aatgatattc gaatacgctt tgaggagata cagcctaata tccgacaaac tgttttacag
     6421 atttacgatc gtacttgtta cccatcattg aattttgaac atccgaacct gggagttttc
     6481 cctgaaacag atagtatatt tgaacctgta taataatata tagtctagcg ctttacggaa
     6541 gacaatgtat gtatttcggt tcctggagaa actattgcat ctattgcata ggtaatcttg
     6601 cacgtcgcat ccccggttca ttttctgcgt ttccatcttg cacttcaata gcatatcttt
     6661 gttaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     6721 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     6781 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgacgagag
     6841 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     6901 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
     6961 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
     7021 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
     7081 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
     7141 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
     7201 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
     7261 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
     7321 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
     7381 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
     7441 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     7501 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatgggaag ctccaccccg
     7561 gttgataatc agaaaagccc caaaaacagg aagattatta tcaaaaagga tcttcaccta
     7621 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7681 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7741 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agcgcttacc
     7801 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7861 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7921 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7981 tttgcgcaac gttgttggca ttgctacagg catcgtggtg tcactctcgt cgtttggtat
     8041 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8101 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8161 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8221 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8281 accgagttgc tcttgcccgg cgtcaatacg ggataatagt gtatcacata gcagaacttt
     8341 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8401 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8461 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8521 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8581 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8641 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gctaagaaac cattattatc
     8701 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//