LOCUS       pYPK0_tp_gene_tp        9223 bp    DNA     circular UNK 09-DEC-2014
DEFINITION  pYPK0_PGItp_ScXKS1_FBA1tp
ACCESSION   -
VERSION     -
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     overlap         363..486
                     /note="olp_GqXRnFdj0bUssSD9FyJPEMLnbh0"
                     /ApEinfo_revcolor="#B0DACE"
                     /chksum="GqXRnFdj0bUssSD9FyJPEMLnbh0"
                     /ApEinfo_fwdcolor="#C4B0D1"
     misc            531..557
                     /label="549_ScPGI1tpf"
     primer_bind     537..557
                     /note="549_ScPGI1tpf"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     gene            807..911
                     /locus_tag="YBR196C-B"
     mRNA            807..911
                     /locus_tag="YBR196C-B"
                     /product="hypothetical protein"
     CDS             807..911
                     /product="hypothetical protein"
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     ND]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     ND]"
                     /codon_start=1
                     /locus_tag="YBR196C-B"
                     /note="hypothetical protein; identified by expression
                     profiling and mass spectrometry"
                     /db_xref="SGD:S000028816"
                     /GO_process="GO:0008150 - biological_process [Evidence ND]"
                     /translation="MWVVLSKEKILLKKAYYAKTILFSALVLRGVRGE"
                     /protein_id="DAA07312.1"
     gene            1264..1413
                     /locus_tag="YBR196C-A"
     mRNA            1264..1413
                     /locus_tag="YBR196C-A"
                     /product="hypothetical protein"
     CDS             1264..1413
                     /product="hypothetical protein"
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     ND]"
                     /GO_component="GO:0016021 - integral to membrane [Evidence
                     IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA,IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     ND]"
                     /codon_start=1
                     /locus_tag="YBR196C-A"
                     /note="hypothetical protein; identified by fungal homology
                     and RT-PCR"
                     /db_xref="SGD:S000028534"
                     /GO_process="GO:0008150 - biological_process [Evidence ND]"
                     /translation="MSRVYIYPLTVFYFFAIEMSVFCYYNWFYRRNFPYLFRPIFPFLI
                     VLIS"
                     /protein_id="DAA07311.1"
     primer_bind     complement(1516..1536)
                     /note="622_ScPGI1tpr_PacI"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(1516..1543)
                     /label="622_ScPGI1tpr_PacI"
     overlap         1544..1593
                     /note="olp_8ro7GZnjXs9RDyEisndJYyFeLIU"
                     /ApEinfo_revcolor="#F0B2E6"
                     /chksum="8ro7GZnjXs9RDyEisndJYyFeLIU"
                     /ApEinfo_fwdcolor="#D4F6C4"
     primer_bind     complement(1571..1591)
                     /note="567_pCAPsAjiIF"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(1571..1593)
                     /label="567_pCAPsAjiIF"
     primer_bind     complement(1571..1593)
                     /note="567"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(1571..1593)
                     /label="567"
     misc            1594..1615
                     /label="pfw1803"
     primer_bind     1596..1615
                     /note="pfw1803"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(3379..3398)
                     /note="prv1803"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(3379..3398)
                     /label="prv1803"
     overlap         3399..3435
                     /note="olp_+ALddA7RDRqA4MsR6S2vDiSMYD0"
                     /ApEinfo_revcolor="#F6CBC7"
                     /chksum="+ALddA7RDRqA4MsR6S2vDiSMYD0"
                     /ApEinfo_fwdcolor="#FABFB1"
     primer_bind     complement(3405..3429)
                     /note="467"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(3405..3435)
                     /label="467"
     primer_bind     3436..3458
                     /note="pfw630"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(4045..4065)
                     /note="prv630"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(4045..4072)
                     /label="prv630"
     overlap         4126..4367
                     /note="olp_WozPxSoJjWK29v8yQeT4MATRraA"
                     /ApEinfo_revcolor="#F4D1E8"
                     /chksum="WozPxSoJjWK29v8yQeT4MATRraA"
                     /ApEinfo_fwdcolor="#FACDE6"
     primer_bind     complement(4339..4367)
                     /note="578"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(4339..4367)
                     /label="578"
     rep_origin      complement(4756..5438)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="gray50"
                     /label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
     misc_feature    5440..6498
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="green"
                     /label="URA3"
                     /ApEinfo_fwdcolor="cyan"
     CDS             complement(8170..8829)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="yellow"
                     /label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
      241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
      301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
      361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
      421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
      481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac ttaaataatt
      541 cagttttctg actgagttaa aaactaatgt agcgacacca cttccattgg tgcctccgtt
      601 attttttttt tgtatgttaa tgctaaataa tacaccgcta tgtatttcag ggcactactt
      661 ctacacatca acggtactaa acatttcgct caagatcggt ccgctttcac gtatttggat
      721 gctatgcaat gttgactatt cttagtggat aacatgcggc atttcctggc ggctatttgc
      781 atcgctatgt aagtggcaat gttcggatgt gggtagtact ttcaaaagaa aaaatattac
      841 tgaagaaggc atactacgcc aagactattt tattctcagc acttgtccta cgtggggtta
      901 gaggcgagta agactttggc cccgctgcgg aagcaaagag aattttgcca tcggacatgc
      961 taccttacgc ttatatctct cattggaata tcgttttctg attaaaacac ggaagtaaga
     1021 acttaattcg tttttcgttg aactatgttg tgccagcgta acattaaaaa agagtgtaca
     1081 aggccacgtt ctgtcaccgt cagaaaaata tgtcaatgag gcaagaaccg ggatggtaac
     1141 aaaaatcacg atctgggtgg gtgtgggtgt attggattat aggaagccac gcgctcaacc
     1201 tggaattaca ggaagctggt aattttttgg gtttgcaatc atcaccatct gcacgttgtt
     1261 ataatgtccc gtgtctatat atatccattg acggtattct atttttttgc tattgaaatg
     1321 agcgtttttt gttactacaa ttggttttac agacggaatt ttccctattt gtttcgtccc
     1381 atttttcctt ttctcattgt tctcatatct taaaaaggtc ctttcttcat aatcaatgct
     1441 ttcttttact taatatttta cttgcattca gtgaatttta atacatattc ctctagtctt
     1501 gcaaaatcga tttagaatca agataccagc ctaaaattaa ttagtcgagg aacgccaggt
     1561 tgcccacttt ctcactagtg acctgcagcc gacaaatgtt gtgttcagta attcagagac
     1621 agacaagaga ggtttccaac acaatgtctt tagactcata ctatcttggg tttgatcttt
     1681 cgacccaaca actgaaatgt ctcgccatta accaggacct aaaaattgtc cattcagaaa
     1741 cagtggaatt tgaaaaggat cttccgcatt atcacacaaa gaagggtgtc tatatacacg
     1801 gcgacactat cgaatgtccc gtagccatgt ggttagaggc tctagatctg gttctctcga
     1861 aatatcgcga ggctaaattt ccattgaaca aagttatggc cgtctcaggg tcctgccagc
     1921 agcacgggtc tgtctactgg tcctcccaag ccgaatctct gttagagcaa ttgaataaga
     1981 aaccggaaaa agatttattg cactacgtga gctctgtagc atttgcaagg caaaccgccc
     2041 ccaattggca agaccacagt actgcaaagc aatgtcaaga gtttgaagag tgcataggtg
     2101 ggcctgaaaa aatggctcaa ttaacagggt ccagagccca ttttagattt actggtcctc
     2161 aaattctgaa aattgcacaa ttagaaccag aagcttacga aaaaacaaag accatttctt
     2221 tagtgtctaa ttttttgact tctatcttag tgggccatct tgttgaatta gaggaggcag
     2281 atgcctgtgg tatgaacctt tatgatatac gtgaaagaaa attcagtgat gagctactac
     2341 atctaattga tagttcttct aaggataaaa ctatcagaca aaaattaatg agagcaccca
     2401 tgaaaaattt gatagcgggt accatctgta aatattttat tgagaagtac ggtttcaata
     2461 caaactgcaa ggtctctccc atgactgggg ataatttagc cactatatgt tctttacccc
     2521 tgcggaagaa tgacgttctc gtttccctag gaacaagtac tacagttctt ctggtcaccg
     2581 ataagtatca cccctctccg aactatcatc ttttcattca tccaactctg ccaaaccatt
     2641 atatgggtat gatttgttat tgtaatggtt ctttggcaag ggagaggata agagacgagt
     2701 taaacaaaga acgggaaaat aattatgaga agactaacga ttggactctt tttaatcaag
     2761 ctgtgctaga tgactcagaa agtagtgaaa atgaattagg tgtatatttt cctctggggg
     2821 agatcgttcc tagcgtaaaa gccataaaca aaagggttat cttcaatcca aaaacgggta
     2881 tgattgaaag agaggtggcc aagttcaaag acaagaggca cgatgccaaa aatattgtag
     2941 aatcacaggc tttaagttgc agggtaagaa tatctcccct gctttcggat tcaaacgcaa
     3001 gctcacaaca gagactgaac gaagatacaa tcgtgaagtt tgattacgat gaatctccgc
     3061 tgcgggacta cctaaataaa aggccagaaa ggactttttt tgtaggtggg gcttctaaaa
     3121 acgatgctat tgtgaagaag tttgctcaag tcattggtgc tacaaagggt aattttaggc
     3181 tagaaacacc aaactcatgt gcccttggtg gttgttataa ggccatgtgg tcattgttat
     3241 atgactctaa taaaattgca gttccttttg ataaatttct gaatgacaat tttccatggc
     3301 atgtaatgga aagcatatcc gatgtggata atgaaaattg ggatcgctat aattccaaga
     3361 ttgtcccctt aagcgaactg gaaaagactc tcatctaagt gccatctgtg cagacaaacg
     3421 catcaggatt taaatataac aatactgaca gtactaaata attgcctact tggcttcaca
     3481 tacgttgcat acgtcgatat agataataat gataatgaca gcaggattat cgtaatacgt
     3541 aatagttgaa aatctcaaaa atgtgtgggt cattacgtaa ataatgatag gaatgggatt
     3601 cttctatttt tcctttttcc attctagcag ccgtcgggaa aacgtggcat cctctctttc
     3661 gggctcaatt ggagtcacgc tgccgtgagc atcctctctt tccatatcta acaactgagc
     3721 acgtaaccaa tggaaaagca tgagcttagc gttgctccaa aaaagtattg gatggttaat
     3781 accatttgtc tgttctcttc tgactttgac tcctcaaaaa aaaaaaatct acaatcaaca
     3841 gatcgcttca attacgccct cacaaaaact tttttccttc ttcttcgccc acgttaaatt
     3901 ttatccctca tgttgtctaa cggatttctg cacttgattt attataaaaa gacaaagaca
     3961 taatacttct ctatcaattt cagttattgt tcttccttgc gttattcttc tgttcttctt
     4021 tttcttttgt catatataac cataaccaag taatacatat tcaaattaat taatccggat
     4081 ttacctgaat caattggcga aattttttgt acgaaatttc agccacttca caggcggttt
     4141 tcgcacgtac ccatgcgcta cgttcctggc cctcttcaaa caggcccagt tcgccaataa
     4201 aatcaccctg attcagatag gagaggatca tttctttacc ctcttcgtct ttgatcagca
     4261 ctgccacaga gcctttaacg atgtagtaca gcgtttccgc tttttcaccc tggtgaataa
     4321 gcgtgctctt ggatgggtac ttatgaatgt ggcaatgaga caagaaccat tcgagagtag
     4381 gatccgtttg aggtttacca agtaccataa gatccttaaa tttttattat ctagctagat
     4441 gataatatta tatcaagaat tgtacctgaa agcaaataaa ttttttatct ggcttaacta
     4501 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     4561 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     4621 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4681 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4741 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4801 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4861 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4921 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4981 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5041 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5101 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5161 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     5221 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5281 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5341 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5401 gtggaacgaa aactcacgtt aagggatttt ggtcatgagg ggtaataact gatataatta
     5461 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
     5521 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
     5581 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
     5641 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
     5701 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
     5761 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
     5821 acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
     5881 aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc
     5941 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc
     6001 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
     6061 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
     6121 atcagtcaaa atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
     6181 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
     6241 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
     6301 cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag
     6361 ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac
     6421 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
     6481 aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg
     6541 aaaagctagc ttatcgatga taagctgtca aagatgagaa ttaattccac ggactataga
     6601 ctatactaga tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac
     6661 gaggccttac cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta
     6721 agattctatc ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa
     6781 aaggcacttc tacaatggct gccatcatta ttatccgatg tgacgctgca gcttctcaat
     6841 gatattcgaa tacgctttga ggagatacag cctaatatcc gacaaactgt tttacagatt
     6901 tacgatcgta cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct
     6961 gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac
     7021 aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac
     7081 gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt
     7141 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
     7201 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
     7261 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg acgagagcgc
     7321 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     7381 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     7441 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     7501 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     7561 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     7621 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     7681 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     7741 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     7801 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     7861 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     7921 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     7981 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tgggaagctc caccccggtt
     8041 gataatcaga aaagccccaa aaacaggaag attattatca aaaaggatct tcacctagat
     8101 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8161 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8221 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagc gcttaccatc
     8281 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8341 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8401 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8461 gcgcaacgtt gttggcattg ctacaggcat cgtggtgtca ctctcgtcgt ttggtatggc
     8521 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8581 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8641 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8701 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8761 gagttgctct tgcccggcgt caatacggga taatagtgta tcacatagca gaactttaaa
     8821 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8881 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8941 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9001 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9061 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9121 aggggttccg cgcacatttc cccgaaaagt gccacctgct aagaaaccat tattatcatg
     9181 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//