LOCUS       pYPK0_tp_gene_tp        8580 bp    DNA     circular UNK 09-DEC-2014
DEFINITION  pYPK0_TDH3tp_SsXYL2_PGItp
ACCESSION   -
VERSION     -
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     overlap         363..486
                     /note="olp_GqXRnFdj0bUssSD9FyJPEMLnbh0"
                     /ApEinfo_revcolor="#BAE5EA"
                     /chksum="GqXRnFdj0bUssSD9FyJPEMLnbh0"
                     /ApEinfo_fwdcolor="#D7CFE4"
     misc            531..555
                     /label="pfw698"
     primer_bind     537..555
                     /note="pfw698"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(1215..1234)
                     /note="prv698"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(1215..1241)
                     /label="prv698"
     overlap         1242..1291
                     /note="olp_8ro7GZnjXs9RDyEisndJYyFeLIU"
                     /ApEinfo_revcolor="#F2B0F4"
                     /chksum="8ro7GZnjXs9RDyEisndJYyFeLIU"
                     /ApEinfo_fwdcolor="#EAFCB9"
     primer_bind     complement(1269..1291)
                     /note="567"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(1269..1291)
                     /label="567"
     misc            1292..1310
                     /label="pfw1092"
     primer_bind     1294..1310
                     /note="pfw1092"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     primer_bind     complement(2370..2385)
                     /note="prv1092"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(2370..2385)
                     /label="prv1092"
     overlap         2386..2422
                     /note="olp_+ALddA7RDRqA4MsR6S2vDiSMYD0"
                     /ApEinfo_revcolor="#BBD1F9"
                     /chksum="+ALddA7RDRqA4MsR6S2vDiSMYD0"
                     /ApEinfo_fwdcolor="#F3F2CB"
     primer_bind     complement(2392..2416)
                     /note="467"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(2392..2422)
                     /label="467"
     primer_bind     2423..2443
                     /note="549_ScPGI1tpf"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     gene            2693..2797
                     /locus_tag="YBR196C-B"
     mRNA            2693..2797
                     /locus_tag="YBR196C-B"
                     /product="hypothetical protein"
     CDS             2693..2797
                     /product="hypothetical protein"
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     ND]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     ND]"
                     /codon_start=1
                     /locus_tag="YBR196C-B"
                     /note="hypothetical protein; identified by expression
                     profiling and mass spectrometry"
                     /db_xref="SGD:S000028816"
                     /GO_process="GO:0008150 - biological_process [Evidence ND]"
                     /translation="MWVVLSKEKILLKKAYYAKTILFSALVLRGVRGE"
                     /protein_id="DAA07312.1"
     gene            3150..3299
                     /locus_tag="YBR196C-A"
     mRNA            3150..3299
                     /locus_tag="YBR196C-A"
                     /product="hypothetical protein"
     CDS             3150..3299
                     /product="hypothetical protein"
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     ND]"
                     /GO_component="GO:0016021 - integral to membrane [Evidence
                     IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA,IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     ND]"
                     /codon_start=1
                     /locus_tag="YBR196C-A"
                     /note="hypothetical protein; identified by fungal homology
                     and RT-PCR"
                     /db_xref="SGD:S000028534"
                     /GO_process="GO:0008150 - biological_process [Evidence ND]"
                     /translation="MSRVYIYPLTVFYFFAIEMSVFCYYNWFYRRNFPYLFRPIFPFLI
                     VLIS"
                     /protein_id="DAA07311.1"
     primer_bind     complement(3402..3422)
                     /note="622_ScPGI1tpr_PacI"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_fwdcolor="green"
     misc            complement(3402..3429)
                     /label="622_ScPGI1tpr_PacI"
     overlap         3483..3724
                     /note="olp_WozPxSoJjWK29v8yQeT4MATRraA"
                     /ApEinfo_revcolor="#E1B9FE"
                     /chksum="WozPxSoJjWK29v8yQeT4MATRraA"
                     /ApEinfo_fwdcolor="#E2ECEE"
     primer_bind     complement(3696..3724)
                     /note="578"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     misc            complement(3696..3724)
                     /label="578"
     rep_origin      complement(4113..4795)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="gray50"
                     /label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
     misc_feature    4797..5855
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="green"
                     /label="URA3"
                     /ApEinfo_fwdcolor="cyan"
     CDS             complement(7527..8186)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_revcolor="yellow"
                     /label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
      241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
      301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
      361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
      421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
      481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac ttaaatataa
      541 aaaacacgct ttttcagttc gagtttatca ttatcaatac tgccatttca aagaatacgt
      601 aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag ccttttaatt
      661 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcgt
      721 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt
      781 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcaccaacc
      841 atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac aaacaggcaa
      901 aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga tgacacaagg
      961 caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc ttctgctctc
     1021 tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat tattccccta
     1081 cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct
     1141 taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa acaccaagaa
     1201 cttagtttcg aataaacaca cataaacaaa caaattaatt agtcgaggaa cgccaggttg
     1261 cccactttct cactagtgac ctgcagccga caaatgactg ctaacccttc cttggtgttg
     1321 aacaagatcg acgacatttc gttcgaaact tacgatgccc cagaaatctc tgaacctacc
     1381 gatgtcctcg tccaggtcaa gaaaaccggt atctgtggtt ccgacatcca cttctacgcc
     1441 catggtagaa tcggtaactt cgttttgacc aagccaatgg tcttgggtca cgaatccgcc
     1501 ggtactgttg tccaggttgg taagggtgtc acctctctta aggttggtga caacgtcgct
     1561 atcgaaccag gtattccatc cagattctcc gacgaataca agagcggtca ctacaacttg
     1621 tgtcctcaca tggccttcgc cgctactcct aactccaagg aaggcgaacc aaacccacca
     1681 ggtaccttat gtaagtactt caagtcgcca gaagacttct tggtcaagtt gccagaccac
     1741 gtcagcttgg aactcggtgc tcttgttgag ccattgtctg ttggtgtcca cgcctccaag
     1801 ttgggttccg ttgctttcgg cgactacgtt gccgtctttg gtgctggtcc tgttggtctt
     1861 ttggctgctg ctgtcgccaa gaccttcggt gctaagggtg tcatcgtcgt tgacattttc
     1921 gacaacaagt tgaagatggc caaggacatt ggtgctgcta ctcacacctt caactccaag
     1981 accggtggtt ctgaagaatt gatcaaggct ttcggtggta acgtgccaaa cgtcgttttg
     2041 gaatgtactg gtgctgaacc ttgtatcaag ttgggtgttg acgccattgc cccaggtggt
     2101 cgtttcgttc aagttggtaa cgctgctggt ccagtcagct tcccaatcac cgttttcgcc
     2161 atgaaggaat tgactttgtt cggttctttc agatacggat tcaacgacta caagactgct
     2221 gttggaatct ttgacactaa ctaccaaaac ggtagagaaa atgctccaat tgactttgaa
     2281 caattgatca cccacagata caagttcaag gacgctattg aagcctacga cttggtcaga
     2341 gccggtaagg gtgctgtcaa gtgtctcatt gacggccctg agtaagtgcc atctgtgcag
     2401 acaaacgcat caggatttaa ataattcagt tttctgactg agttaaaaac taatgtagcg
     2461 acaccacttc cattggtgcc tccgttattt tttttttgta tgttaatgct aaataataca
     2521 ccgctatgta tttcagggca ctacttctac acatcaacgg tactaaacat ttcgctcaag
     2581 atcggtccgc tttcacgtat ttggatgcta tgcaatgttg actattctta gtggataaca
     2641 tgcggcattt cctggcggct atttgcatcg ctatgtaagt ggcaatgttc ggatgtgggt
     2701 agtactttca aaagaaaaaa tattactgaa gaaggcatac tacgccaaga ctattttatt
     2761 ctcagcactt gtcctacgtg gggttagagg cgagtaagac tttggccccg ctgcggaagc
     2821 aaagagaatt ttgccatcgg acatgctacc ttacgcttat atctctcatt ggaatatcgt
     2881 tttctgatta aaacacggaa gtaagaactt aattcgtttt tcgttgaact atgttgtgcc
     2941 agcgtaacat taaaaaagag tgtacaaggc cacgttctgt caccgtcaga aaaatatgtc
     3001 aatgaggcaa gaaccgggat ggtaacaaaa atcacgatct gggtgggtgt gggtgtattg
     3061 gattatagga agccacgcgc tcaacctgga attacaggaa gctggtaatt ttttgggttt
     3121 gcaatcatca ccatctgcac gttgttataa tgtcccgtgt ctatatatat ccattgacgg
     3181 tattctattt ttttgctatt gaaatgagcg ttttttgtta ctacaattgg ttttacagac
     3241 ggaattttcc ctatttgttt cgtcccattt ttccttttct cattgttctc atatcttaaa
     3301 aaggtccttt cttcataatc aatgctttct tttacttaat attttacttg cattcagtga
     3361 attttaatac atattcctct agtcttgcaa aatcgattta gaatcaagat accagcctaa
     3421 aattaattaa tccggattta cctgaatcaa ttggcgaaat tttttgtacg aaatttcagc
     3481 cacttcacag gcggttttcg cacgtaccca tgcgctacgt tcctggccct cttcaaacag
     3541 gcccagttcg ccaataaaat caccctgatt cagataggag aggatcattt ctttaccctc
     3601 ttcgtctttg atcagcactg ccacagagcc tttaacgatg tagtacagcg tttccgcttt
     3661 ttcaccctgg tgaataagcg tgctcttgga tgggtactta tgaatgtggc aatgagacaa
     3721 gaaccattcg agagtaggat ccgtttgagg tttaccaagt accataagat ccttaaattt
     3781 ttattatcta gctagatgat aatattatat caagaattgt acctgaaagc aaataaattt
     3841 tttatctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     3901 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     3961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4561 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaggggt
     4801 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact
     4861 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct
     4921 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca
     4981 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc
     5041 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct
     5101 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc
     5161 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac
     5221 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc
     5281 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct
     5341 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat
     5401 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact
     5461 gtgccctcca tggaaaaatc agtcaaaata tccacatgtg tttttagtaa acaaattttg
     5521 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca
     5581 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga
     5641 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcag
     5701 gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta
     5761 catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg
     5821 gagattaccg aatcaaaaaa atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa
     5881 aatgatgaat tgaattgaaa agctagctta tcgatgataa gctgtcaaag atgagaatta
     5941 attccacgga ctatagacta tactagatac tccgtctact gtacgataca cttccgctca
     6001 ggtccttgtc ctttaacgag gccttaccac tcttttgtta ctctattgat ccagctcagc
     6061 aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt aaaactagct agaccgagaa
     6121 agagactaga aatgcaaaag gcacttctac aatggctgcc atcattatta tccgatgtga
     6181 cgctgcagct tctcaatgat attcgaatac gctttgagga gatacagcct aatatccgac
     6241 aaactgtttt acagatttac gatcgtactt gttacccatc attgaatttt gaacatccga
     6301 acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa tatatagtct
     6361 agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt gcatctattg
     6421 cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat cttgcacttc
     6481 aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg
     6541 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     6601 acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg
     6661 caacgcgacg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
     6721 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta
     6781 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct
     6841 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg
     6901 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt
     6961 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc
     7021 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt
     7081 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact
     7141 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta
     7201 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga
     7261 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
     7321 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatgg
     7381 gaagctccac cccggttgat aatcagaaaa gccccaaaaa caggaagatt attatcaaaa
     7441 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7501 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7561 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7621 cgggagcgct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7681 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7741 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7801 tcgccagtta atagtttgcg caacgttgtt ggcattgcta caggcatcgt ggtgtcactc
     7861 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7921 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7981 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8041 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8101 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa tagtgtatca
     8161 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8221 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8281 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8341 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8401 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8461 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgctaag
     8521 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//