LOCUS       pYPKa_A_ScXKS1          4933 bp    DNA     circular UNK 14-MAY-2014
DEFINITION  pYPKa_A_ScXKS1
ACCESSION   pYPKa_A_ScXKS1
VERSION     pYPKa_A_ScXKS1
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    528..533
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#002cff"
                     /label="ZraI"
                     /ApEinfo_revcolor="green"
     primer_bind     583..602
                     /note="pfw1803"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     primer_bind     complement(2366..2385)
                     /note="prv1803"
                     /ApEinfo_fwdcolor="green"
                     /ApEinfo_revcolor="red"
     protein_bind    2414..2419
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#002cff"
                     /label="EcoRV"
                     /ApEinfo_revcolor="green"
     rep_origin      3118
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /direction=BOTH
                     /ApEinfo_revcolor="pink"
                     /label="rep_origin"
     gene            complement(3877..4737)
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_fwdcolor="#ffc02c"
                     /gene="bla"
                     /label="bla"
                     /ApEinfo_revcolor="pink"
     CDS             complement(3877..4737)
                     /product="beta-lactamase"
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /db_xref="GI:2769263"
                     /db_xref="GOA:Q79DR3"
                     /db_xref="HSSP:P62593"
                     /db_xref="InterPro:IPR000871"
                     /db_xref="InterPro:IPR001466"
                     /db_xref="InterPro:IPR012338"
                     /db_xref="UniProtKB/TrEMBL:Q79DR3"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /label="beta-lactamase"
                     /protein_id="CAA04868.1"
                     /ApEinfo_fwdcolor="pink"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc ctgaggatcg gggtgataaa tcagtctgcg ccacatcggg ggaaacaaaa
      241 tggcgcgaga tctaaaaaaa aaggctccaa aaggagcctt tcgcgctacc aggtaacgcg
      301 ccactccgac gggattaacg agtgccgtaa acgacgatgg ttttaccgtg tgcggagatc
      361 aggttctgat cctcgagcat cttaagaatt cgtcccacgg tttgtctaga gcagccgaca
      421 atctggccaa tttcctgacg ggtaattttg atttgcatgc cgtccgggtg agtcatagcg
      481 tctggttgtt ttgccagatt cagcagagtc tgtgcaatgc ggccgctgac gtcgaggaac
      541 gccaggttgc ccactttctc actagtgacc tgcagccgac aaatgttgtg ttcagtaatt
      601 cagagacaga caagagaggt ttccaacaca atgtctttag actcatacta tcttgggttt
      661 gatctttcga cccaacaact gaaatgtctc gccattaacc aggacctaaa aattgtccat
      721 tcagaaacag tggaatttga aaaggatctt ccgcattatc acacaaagaa gggtgtctat
      781 atacacggcg acactatcga atgtcccgta gccatgtggt tagaggctct agatctggtt
      841 ctctcgaaat atcgcgaggc taaatttcca ttgaacaaag ttatggccgt ctcagggtcc
      901 tgccagcagc acgggtctgt ctactggtcc tcccaagccg aatctctgtt agagcaattg
      961 aataagaaac cggaaaaaga tttattgcac tacgtgagct ctgtagcatt tgcaaggcaa
     1021 accgccccca attggcaaga ccacagtact gcaaagcaat gtcaagagtt tgaagagtgc
     1081 ataggtgggc ctgaaaaaat ggctcaatta acagggtcca gagcccattt tagatttact
     1141 ggtcctcaaa ttctgaaaat tgcacaatta gaaccagaag cttacgaaaa aacaaagacc
     1201 atttctttag tgtctaattt tttgacttct atcttagtgg gccatcttgt tgaattagag
     1261 gaggcagatg cctgtggtat gaacctttat gatatacgtg aaagaaaatt cagtgatgag
     1321 ctactacatc taattgatag ttcttctaag gataaaacta tcagacaaaa attaatgaga
     1381 gcacccatga aaaatttgat agcgggtacc atctgtaaat attttattga gaagtacggt
     1441 ttcaatacaa actgcaaggt ctctcccatg actggggata atttagccac tatatgttct
     1501 ttacccctgc ggaagaatga cgttctcgtt tccctaggaa caagtactac agttcttctg
     1561 gtcaccgata agtatcaccc ctctccgaac tatcatcttt tcattcatcc aactctgcca
     1621 aaccattata tgggtatgat ttgttattgt aatggttctt tggcaaggga gaggataaga
     1681 gacgagttaa acaaagaacg ggaaaataat tatgagaaga ctaacgattg gactcttttt
     1741 aatcaagctg tgctagatga ctcagaaagt agtgaaaatg aattaggtgt atattttcct
     1801 ctgggggaga tcgttcctag cgtaaaagcc ataaacaaaa gggttatctt caatccaaaa
     1861 acgggtatga ttgaaagaga ggtggccaag ttcaaagaca agaggcacga tgccaaaaat
     1921 attgtagaat cacaggcttt aagttgcagg gtaagaatat ctcccctgct ttcggattca
     1981 aacgcaagct cacaacagag actgaacgaa gatacaatcg tgaagtttga ttacgatgaa
     2041 tctccgctgc gggactacct aaataaaagg ccagaaagga ctttttttgt aggtggggct
     2101 tctaaaaacg atgctattgt gaagaagttt gctcaagtca ttggtgctac aaagggtaat
     2161 tttaggctag aaacaccaaa ctcatgtgcc cttggtggtt gttataaggc catgtggtca
     2221 ttgttatatg actctaataa aattgcagtt ccttttgata aatttctgaa tgacaatttt
     2281 ccatggcatg taatggaaag catatccgat gtggataatg aaaattggga tcgctataat
     2341 tccaagattg tccccttaag cgaactggaa aagactctca tctaagtgcc atctgtgcag
     2401 acaaacgcat caggatatcc ggatttacct gaatcaattg gcgaaatttt ttgtacgaaa
     2461 tttcagccac ttcacaggcg gttttcgcac gtacccatgc gctacgttcc tggccctctt
     2521 caaacaggcc cagttcgcca ataaaatcac cctgattcag ataggagagg atcatttctt
     2581 taccctcttc gtctttgatc agcactgcca cagagccttt aacgatgtag tacagcgttt
     2641 ccgctttttc accctggtga ataagcgtgc tcttggatgg gtacttatga atgtggcaat
     2701 gagacaagaa ccattcgaga gtaggatccg tttgaggttt accaagtacc ataagatcct
     2761 taaattttta ttatctagct agatgataat attatatcaa gaattgtacc tgaaagcaaa
     2821 taaatttttt atctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     2881 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     2941 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3001 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3061 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3121 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3181 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3241 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3301 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3361 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3421 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3481 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3541 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3601 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3661 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3721 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3781 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3841 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3901 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3961 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4021 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4081 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4141 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4201 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4261 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4321 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4381 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4441 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4501 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4561 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4621 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4681 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4741 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4801 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4861 gccacctgct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4921 aggccctttc gtc
//