usage: Tm [-h] [--uncorrected] [-d DNA] [--na NA] [--mg MG] [--dntp DNTP]
          [--version]
          seq

Calculate nucleotide melting temps

positional arguments:
  seq                nucleotide sequence

optional arguments:
  -h, --help         show this help message and exit
  --uncorrected      Do not use monovalent/divalent cation corrections
  -d DNA, --dna DNA  DNA concentration (nM)
  --na NA            Na+ concentration (mM)
  --mg MG            Mg++ concentration (mM)
  --dntp DNTP        Nucleotide triphosphate concentration (mM)
  --version          show program's version number and exit
