LOCUS                  Newcrap        2963 bp ds-DNA   linear       13-JAN-2011
DEFINITION  .
ACCESSION   .
VERSION     .
SOURCE      .
ORGANISM    .
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..5741
                     /mol_type="other DNA"
                     /organism="YEplac181"
     promoter        142..171
                     /label=lac_promoter
     misc_feature    185..207
                     /label=M13_pUC_rev_primer
     promoter        206..224
                     /label=M13_reverse_primer
     misc_feature    287..442
                     /label=lacZ_a
     promoter        complement(290..306)
                     /label=M13_forward20_primer
     misc_feature    complement(299..321)
                     /label=M13_pUC_fwd_primer
     rep_origin      complement(743..1907)
                     /label=2micron2_origin
     gene            complement(2227..3319)
                     /gene="LEU2 (variant)"
                     /label=LEU2 (variant)
     CDS             complement(2227..2805)
                     /translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT 
                     LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL 
                     PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV 
                     /label=ORF frame 3
     promoter        3870..3898
                     /translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT 
                     LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL 
                     PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV 
                     /label=AmpR_promoter
     gene            3940..4800
                     /label=Ampicillin
                     /translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT 
                     LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL 
                     PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV 
                     /gene="Ampicillin"
     CDS             3940..4800
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     /label=ORF frame 1
     rep_origin      4955..5574
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     /label=pBR322_origin
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
      541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
      601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
      661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
      721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
      781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
      841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
      901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
      961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
     1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
     1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
     1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
     1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
     1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
     2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
     2101 aagctgtcaa acatgagaat taattctacc ctatgaacat attccatttt gtaatttcgt
     2161 gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga
     2221 atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt
     2281 actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc
     2341 atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc
     2401 agacaagata gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccgtg
     2461 gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga
     2521 tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa
     2581 catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc
     2641 ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct tgatggtttc
     2701 ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca
     2761 aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg
     2821 cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt
     2881 tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt
     2941 agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg
     3001 tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct
     3061 aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt
     3121 cttggaggct tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc
     3181 accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt
     3241 aagaacctta atggcttcgg ctgtgatctt gaccaacgtg gtcacctggc aaaacgacga
     3301 tcttcttagg ggcagacata ggggcagaca ttagaatgta tatccttgaa atatatatat
     3361 atattgctga aatgtaaaag gtaagaaaag ttagaataag acgattgcta accacctatt
     3421 ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt
     3481 tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcctctc acctttcctt
     3541 tttctcccaa tttttcagtt gaaaaaggta tatgcgcggc gacctctgaa attaacaaaa
     3601 aatttccagt catcgaattt gtctgtgcga tagcgcccct gtgtgttctc gttatgttga
     3661 ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt
     3721 cccacagtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3781 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3841 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3901 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3961 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4021 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4081 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4141 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4201 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4261 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4321 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4381 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4441 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4501 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4561 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4621 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4681 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4741 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4801 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4861 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5101 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5641 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5701 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//
