LOCUS       YEplac181	5741 bp 	DNA	SYN
DEFINITION  YEplac181
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file was downloaded from the addgene website
COMMENT     https://www.addgene.org/browse/sequence_vdb/4893/
COMMENT     The Genbank header is broken, see definition
COMMENT     https://www.ncbi.nlm.nih.gov/Sitemap/samplerecord.html
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="YEplac181"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     misc_feature    287..442
                     /label="lacZ_a"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     rep_origin      complement(743..1907)
                     /label="2micron2_origin"
     gene            complement(2227..3319)
                     /label="LEU2 (variant)"
                     /gene="LEU2 (variant)"
     CDS             complement(2227..2805)
                     /label="ORF frame 3"
                     /translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT
                     LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL
                     PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV
                     LDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     promoter        3870..3898
                     /label="AmpR_promoter"
                     /translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT
                     LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL
                     PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV
                     LDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     gene            3940..4800
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT
                     LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL
                     PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV
                     LDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     CDS             3940..4800
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4955..5574
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
  541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
  601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
  661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
  721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
  781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
  841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
  901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
  961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
 1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
 1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
 1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
 1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
 1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
 1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
 1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
 1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
 1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
 1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
 1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
 1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
 1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
 1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
 1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
 1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
 1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
 2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
 2101 aagctgtcaa acatgagaat taattctacc ctatgaacat attccatttt gtaatttcgt
 2161 gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga
 2221 atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt
 2281 actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc
 2341 atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc
 2401 agacaagata gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccgtg
 2461 gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga
 2521 tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa
 2581 catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc
 2641 ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct tgatggtttc
 2701 ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca
 2761 aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg
 2821 cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt
 2881 tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt
 2941 agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg
 3001 tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct
 3061 aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt
 3121 cttggaggct tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc
 3181 accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt
 3241 aagaacctta atggcttcgg ctgtgatctt gaccaacgtg gtcacctggc aaaacgacga
 3301 tcttcttagg ggcagacata ggggcagaca ttagaatgta tatccttgaa atatatatat
 3361 atattgctga aatgtaaaag gtaagaaaag ttagaataag acgattgcta accacctatt
 3421 ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt
 3481 tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcctctc acctttcctt
 3541 tttctcccaa tttttcagtt gaaaaaggta tatgcgcggc gacctctgaa attaacaaaa
 3601 aatttccagt catcgaattt gtctgtgcga tagcgcccct gtgtgttctc gttatgttga
 3661 ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt
 3721 cccacagtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
 3781 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
 3841 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
 3901 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
 3961 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
 4021 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
 4081 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
 4141 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
 4201 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
 4261 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
 4321 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
 4381 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
 4441 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
 4501 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
 4561 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
 4621 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
 4681 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
 4741 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
 4801 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
 4861 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
 4921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
 4981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
 5041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
 5101 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
 5161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
 5221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
 5281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
 5341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
 5401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
 5461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
 5521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
 5581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
 5641 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
 5701 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//

