LOCUS       AX023560                8601 bp    DNA     linear   PAT 15-SEP-2000
DEFINITION  Sequence 15 from Patent WO0003008.
ACCESSION   AX023560
VERSION     AX023560.1  GI:10183921
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1
  AUTHORS   Barth,G., Juretzek,T. and Mauersberger,S.
  TITLE     Recombinant haploid or diploid yarrowia lipolytica cells for the
            functional heterologous expression of cytochrome p450 systems
  JOURNAL   Patent: WO 0003008-A2 15 20-JAN-2000;
            UNIV DRESDEN TECH (DE)
FEATURES             Location/Qualifiers
     source          1..8601
                     /organism="synthetic construct"
                     /mol_type="unassigned DNA"
                     /db_xref="taxon:32630"
     promoter        8094..8600
                     /note="Promotor des GAL10-Gens aus Saccharomyces
                     cerevisiae;
                     ringf rmig geschlossene DNA aus Saccharomyces
                     cerevisiae/E. coli auf der Basis des 2 -Plasmid yEP51.
                     Plasmid yEP5117a"
ORIGIN
        1 tcgacaatgt ggctgctcct ggctgtcttt ctgctcaccc tcgcctattt attttggccc
       61 aagaccaagc actctggtgc caagtacccc aggagcctcc catccctgcc cctggtgggc
      121 agcctgccgt tcctccccag acgtggccag cagcacaaga acttcttcaa gctgcaggaa
      181 aaatatggcc ccatctattc ctttcgtttg ggttccaaga cgactgtgat gattggacac
      241 caccagttgg ccagggaggt gcttctcaag aagggcaagg aattctctgg gcgtcccaaa
      301 gtggccactc tagacatcct gtcagacaac caaaagggca ttgcctttgc cgaccatggt
      361 gcccactggc agctgcatcg gaagctggca ctgaatgcct ttgccctgtt caaggatggc
      421 aacctgaagt tagagaagat cattaatcag gaagccaatg tgctctgtga tttcctggcc
      481 acccagcatg gagaggccat agatctgtcc gagcctctct ctctggcggt gaccaacata
      541 atcagcttta tctgcttcaa cttctccttc aagaatgagg atcctgccct gaaggccata
      601 caaaatgtca atgatggcat cctggaggtt ctgagcaagg aagttctgtt agacatattc
      661 cctgtgctga agattttccc cagcaaagcc atggaaaaga tgaagggttg tgttcaaacg
      721 cgaaatgaat tgctgaatga aatccttgaa aaatgtcagg agaacttcag cagtgattcc
      781 atcactaact tgctgcacat actgatccaa gccaaggtga atgcagacaa taacaatgct
      841 ggcccagacc aggattcaaa gctgctttca aatagacaca tgctcgctac tataggggac
      901 atcttcgggg ctggtgtgga gaccaccacg tctgtgataa agtggatcgt ggcctacctg
      961 ctacaccatc cttcgttgaa gaagaggatc caggatgaca ttgaccagat tataggtttc
     1021 aatcgcaccc caaccatcag tgaccggaac cgccttgtcc tgctggaggc gaccatcaga
     1081 gaagtgctcc gaatccggcc tgtggcccct acgctgatcc cccacaaggc tgtcattgac
     1141 tccagcattg gcgaccttac cattgacaaa ggcacagacg ttgtggtcaa cctgtgggca
     1201 ctgcatcaca gtgagaagga gtggcagcat cccgacctgt tcatgcccga gcgcttcttg
     1261 gaccccacgg ggacgcaact catctcgcca tcattaagct acttgccctt tggagcagga
     1321 ccccgctcct gcgtaggtga gatgctagcc cgccaggagc tcttcctctt catgtcccgg
     1381 ctgctgcaga ggttcaacct ggagatcccg gatgatggga agctaccttc tctggagggc
     1441 catgccagtc tcgtcttgca gatcaaacct ttcaaggtga agatcgaggt gcgccaggcc
     1501 tggaaggaag cccaggctga gggtagcacc ccatgactcc accctatgtg acccccaccg
     1561 cacagaatta gaggagctcc cccaccctct cccaccattc cttcttcctc cccgcccact
     1621 ctgccttctt tcccagcctg cagccctggc agtgatgtgc attaaacagt ttctttctcc
     1681 aaaacaagct ttgaagaaaa atgcgcctta ttcaatcttt gctataaaaa atggcccaaa
     1741 atctcacatt ggaagacatt tgatgacctc atttctttca atgaagggcc taacggagtt
     1801 gactaatgtt gtgggaaatt ggagcgataa gcgtgcttct gccgtggcca ggacaacgta
     1861 tactcatcag ataacagcaa tacctgatca ctacttcgca ctagtttctc ggtactatgc
     1921 atatgatcca atatcaaagg aaatgatagc attgaaggat gagactaatc caattgagga
     1981 gtggcagcat atagaacagc taaagggtag tgctgaagga agcatacgat accccgcatg
     2041 gaatgggata atatcacagg aggtactaga ctacctttca tcctacataa atagacgcat
     2101 ataagtacgc atttaaagca taaacacgca ctatgccgtt cttctcatgt atatatatat
     2161 acaggcaaca cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc gcagctcgcg
     2221 ttgcattttc ggaagcgctc gttttcggaa acgctttgaa gttcctattc cgaagttcct
     2281 attctctaga aagtatagga acttcagagc gcgttttgaa aaccaaaagc gctctgaaga
     2341 cgcactttca aaaaaccaaa acgcaccgga ctgtaacgag ctactaaaat attgcgaata
     2401 ccgcttccac aaacattgct caaaagtagc tctttgctat atatctctgt gctatatccc
     2461 tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc gacctctaca
     2521 ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta agaactattc
     2581 atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag tatatagaga
     2641 caaaatagaa gaaaccgttc ataaaaaact gaccaatgaa gaatcatcaa cgctatcact
     2701 ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg gatgccttta
     2761 tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa gtggagtcag
     2821 gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc ttaacggacc
     2881 tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag aaaaaaagta
     2941 atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta gaacaaaaaa
     3001 gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa
     3061 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca
     3121 aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg
     3181 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt
     3241 ctacaaaatg aagcacagat gcttcgttaa caaagatatg ctaatgaagt gcaagatgga
     3301 aacgcagaaa atgaaccggg gatgcgacgt gcaagattac ctatgcaata gatgcaatag
     3361 tttctccagg aaccgaaata catacattgt cttccgtaaa gcgctagact atatattatt
     3421 atacaggttc aaatatacta tctgtttcag ggaaaactcc caggttcgga tgttcaaaat
     3481 tcaatgtagg gtaacaagta cgatcgtaaa tctgtaaaac agtttgtcgg atattaggct
     3541 gtatctcctc aaagcgtatt caatatcatt gagaagctgc agcgtcacat cggataataa
     3601 tgatggcagc catggtagaa gtgccttttg catttctagt ctctttctcg gtctagctag
     3661 ttttactaca tcgcgaagat cgaatcttag atcacactgc ctttgctgag ctggatcaat
     3721 agagtaacaa aagagtggta aggcctcgtt aaaggacaag gacctgagcg gaagtgtatc
     3781 gtacagtaga cggagtatac tagtatagtc tatagtccgt ggaattcttg aagacgaaag
     3841 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3901 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3961 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4021 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4081 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4141 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4201 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4261 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4321 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4381 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4441 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4501 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4561 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4621 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4681 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4741 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4801 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4861 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4921 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4981 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5041 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5101 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5161 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     5221 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5281 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5341 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5401 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5461 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5521 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5581 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5641 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5701 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5761 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5821 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     5881 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     5941 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     6001 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6061 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcaga
     6121 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat
     6181 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
     6241 actggaaatt ttttgttaat tcagaggtcg cctgacgcat ataccttttt caactgaaaa
     6301 attgggagaa aaaggaaggt gagaggccgg aaccggtttt catatagaat agagaagcgt
     6361 tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt
     6421 gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta ccttttacat
     6481 ttcagcaata tatatatata tttcaaggat ataccattct aatgtctgcc cctaagaaga
     6541 tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc
     6601 ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg
     6661 gtggtgctgc tatcgatgct acaggtgtcc cacttccaga tgaggcgctg gaagcctcca
     6721 agaaggttga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg
     6781 ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa
     6841 gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg
     6901 ctaaaggtac tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga
     6961 gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag
     7021 tccaaagaat cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta
     7081 tttggtcctt ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg
     7141 aggaaaccat caagaacgaa ttccctacat tgaaggttca acatcaattg attgattctg
     7201 ccgccatgat cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca
     7261 tgtttggtga tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc
     7321 catctgcgtc cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat
     7381 gccacggttc tgctccagat ttgccaaaga ataaggttga ccctatcgcc actatcttgt
     7441 ctgctgcaat gatgttgaaa ttgtcattga acttgcctga agaagctaag gccattgaac
     7501 atgcagttac aaaaggtttt ggatgcaggt atcagaactg gtcatttagg tggttccaac
     7561 agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa
     7621 gattctctct tttttttatg atatttgtac ataaacttta taaatgaaat tcataataga
     7681 aacgacacga aattacaaaa tggaatatgt tcatagggta gacgaaacta tatacgcaat
     7741 ctacatacat ttatcaagaa ggagaaaaag gaggatagta aaggaataca ggtaagcaaa
     7801 ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac
     7861 aaggaggagg gcaccacaaa aagttaggtg taacagaaaa atcatgaaac tacgattcct
     7921 aatttgatat tggaggattt tctctaaaaa aaaaaaaata caacaaataa aaaacactca
     7981 atgacctgac catttgatgg agtttaagtc aataccttct tgaaccattt cccataatgg
     8041 tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc ttacgacgta ggatcaaaaa
     8101 tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc gcattatcat
     8161 cctatggttg ttaatttgat tcgttcattt gaaggtttgt ggggccaggt tactgccaat
     8221 ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc ggagcagtgc
     8281 ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agacgaggac gcacggagga
     8341 gagtcttcct tcggagggct gtcacccgct cggcggcttc taatccgtac ttcaatatag
     8401 caatgagcag ttaagcgtat tactgaaagt tccaaagaga aggttttttt aggctaagat
     8461 aatggggctc tttacatttc cacaacatat aagtaagatt agatatggat agttatatgg
     8521 atatgtatat ggtggtaatg ccatgtaata tgattattaa acttctttgc gtccatccaa
     8581 aaaaaaagta agaatttttg g
//