LOCUS       pUC19                   2695 bp ds-DNA     circular     25-APR-2014
DEFINITION  Cloning vector pUC19c, complete sequence.
ACCESSION   L09137 X02514
VERSION     L09137.2  GI:20141090
KEYWORDS    .
SOURCE      Cloning vector pUC19c
  ORGANISM  Cloning vector pUC19c other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Yanisch-Perron,C., Vieira,J. and Messing,J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
   PUBMED   2985470
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
  TITLE     The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
            to facilitate the use of sonicated DNA for nucleotide sequencing
  JOURNAL   Gene 68 (1), 139-149 (1988)
   PUBMED   2851488
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Department of Biochemistry, University of
            Minnesota, St. Paul, MN 55108, USA
REFERENCE   5  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2002) Rutgers, The State University of New
            Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
            Piscataway, NJ 08854-8020, USA
  REMARK    Sequence update by submitter
COMMENT     On Apr 11, 2002 this sequence version replaced gi:209213. These
            data and their annotation were supplied to GenBank by Will Gilbert
            under the auspices of the GenBank Currator Program. pUC19c -
            Cloning vector (beta-galactosidase mRNA on complementary strand)
            ENTRY PUC19C                    #TYPE DNA CIRCULAR TITLE pUC19c -
            Cloning vector               (beta-galactosidase mRNA on
            complementary strand) DATE      03-FEB-1986    #sequence
            16-DEC-1986 ACCESSION VB0033 SOURCE    artificial COLLECTION  ATCC
            37254 REFERENCE    #number    #authors  Norrander J., Kempe T.,
            Messing J.    #journal Gene (1983) 26: 101-106 REFERENCE    #number
            1    #authors  Yanisch-Perron C., Vieira J., Messing J.    #journal
            Gene (1985) 33: 103-119    #comment shows the complete compiled
            sequence REFERENCE    #number  2    #authors  Chambers,S.P., et al.
            #journal Gene (1988) 68: 139-149    #describes mutation at nt1308
            and its effect on copy number REFERENCE    #number    #authors
            Pouwels P.H., Enger-Valk B.E., Brammar W.J.    #book    Cloning
            Vectors, Elsvier 1985 and supplements    #comment  vector I-A-iv-20
            COMMENT    This Sequence was obtained 3-MAR-1986 from J. Messing,
            Waksman    Institute, NJ on floppy disk.    Revised 16-DEC-1986 by
            F. Pfeiffer:    1062/3 'AT' to 'TA' to match revised sequence of
            PBR322    The beta-galactosidase mRNA sequence including the
            multiple    cloning site of M13mp19 is on the strand complementary
            to that    shown. KEYWORDS CROSSREFERENCE    #complement
            VecBase(3):pUC19    #prerevised      GenBank(50):M11662,
            EMBL(11):ARPuc19    #parent      VecBase(3):pUC13,
            VecBase(3):M13mp19, VecSource(3):bGal19 PARENT    Features of
            pUC19c (2686 bp)      residue     source       1- 137  2074-2210
            pBR322     138- 237  2252-2351 pBR322     238- 395  1461-1304 (c)
            Lac-Operon     396- 452    57-   1 (c) polylinker of M13mp19
            455- 682  1298-1071 (c) Lac-Operon     683-2686  2352-4355 pBR322
            Conflict (cfl) and Mutations (mut):         pUC19c  source    mut
            1308  A  G  2977   pBR322   linked to increased copy number    mut
            1942  A  G  3611   pBR322    mut 2243  T  C  3912   pBR322 FEATURE
            1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER
            HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION
            #resistance  Ap    #indicator beta-galactosidase SUMMARY  pUC19c
            #length 2686   #checksum 4465.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Cloning vector pUC19c"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:174689"
                     /label=source:Cloning vector pUC19c
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          424..2695
                     /organism="Cloning vector pUC19c"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:174689"
                     /label=source:Cloning vector pUC19c(1)
                     /ApEinfo_label=source:Cloning vector pUC19c
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccATGTTT
      421 TAAggggatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag
      481 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      541 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      601 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      661 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      721 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      781 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      841 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      901 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      961 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1021 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1081 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1141 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1201 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1261 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1321 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1381 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1441 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1501 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1561 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1621 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1681 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1741 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1801 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1861 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1921 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1981 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2041 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2101 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2161 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2221 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2281 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2341 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2401 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2461 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2521 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2581 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2641 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
