LOCUS       pUC_LAC4                6389 bp ds-DNA     circular     28-JAN-2014
DEFINITION  Cloning vector pUC19c, complete sequence.
ACCESSION   L09137 X02514
VERSION     L09137.2  GI:20141090
KEYWORDS    .
SOURCE      Cloning vector pUC19c
  ORGANISM  Cloning vector pUC19c other sequences; _artificial_ sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Yanisch-Perron,C., Vieira,J. and Messing,J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
   PUBMED   2985470
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
  TITLE     The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
            to facilitate the use of sonicated DNA for nucleotide sequencing
  JOURNAL   Gene 68 (1), 139-149 (1988)
   PUBMED   2851488
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Department of Biochemistry, University of
            Minnesota, St. Paul, MN 55108, USA
REFERENCE   5  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2002) Rutgers, The State University of New
            Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
            Piscataway, NJ 08854-8020, USA
  REMARK    Sequence update by submitter
COMMENT     On Apr 11, 2002 this sequence version replaced gi:209213. These
            data and their annotation were supplied to GenBank by Will Gilbert
            under the auspices of the GenBank Currator Program. pUC19c -
            Cloning vector (beta-galactosidase mRNA on complementary strand)
            ENTRY PUC19C                    #TYPE DNA CIRCULAR TITLE pUC19c -
            Cloning vector               (beta-galactosidase mRNA on
            complementary strand) DATE      03-FEB-1986    #sequence
            16-DEC-1986 ACCESSION VB0033 SOURCE    artificial COLLECTION  ATCC
            37254 REFERENCE    #number    #authors  Norrander J., Kempe T.,
            Messing J.    #journal Gene (1983) 26: 101-106 REFERENCE    #number
            1    #authors  Yanisch-Perron C., Vieira J., Messing J.    #journal
            Gene (1985) 33: 103-119    #comment shows the complete compiled
            sequence REFERENCE    #number  2    #authors  Chambers,S.P., et al.
            #journal Gene (1988) 68: 139-149    #describes mutation at nt1308
            and its effect on copy number REFERENCE    #number    #authors
            Pouwels P.H., Enger-Valk B.E., Brammar W.J.    #book    Cloning
            Vectors, Elsvier 1985 and supplements    #comment  vector I-A-iv-20
            COMMENT    This Sequence was obtained 3-MAR-1986 from J. Messing,
            Waksman    Institute, NJ on floppy disk.    Revised 16-DEC-1986 by
            F. Pfeiffer:    1062/3 'AT' to 'TA' to match revised sequence of
            PBR322    The beta-galactosidase mRNA sequence including the
            multiple    cloning site of M13mp19 is on the strand complementary
            to that    shown. KEYWORDS CROSSREFERENCE    #complement
            VecBase(3):pUC19    #prerevised      GenBank(50):M11662,
            EMBL(11):ARPuc19    #parent      VecBase(3):pUC13,
            VecBase(3):M13mp19, VecSource(3):bGal19 PARENT    Features of
            pUC19c (2686 bp)      residue     source       1- 137  2074-2210
            pBR322     138- 237  2252-2351 pBR322     238- 395  1461-1304 (c)
            Lac-Operon     396- 452    57-   1 (c) polylinker of M13mp19 455-
            682  1298-1071 (c) Lac-Operon     683-2686  2352-4355 pBR322
            Conflict (cfl) and Mutations (mut):         pUC19c  source    mut
            1308  A  G  2977   pBR322   linked to increased copy number    mut
            1942  A  G  3611   pBR322    mut 2243  T  C  3912   pBR322 FEATURE
            1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER
            HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION
            #resistance  Ap    #indicator beta-galactosidase SUMMARY  pUC19c
            #length 2686   #checksum 4465.
COMMENT     
COMMENT     ApEinfo:methylated:1
ORIGIN
        1 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
       61 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1801 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2221 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     2281 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2341 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2401 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     2461 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     2521 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2581 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2641 tcacgacgtt gtaaaacgac ggccagtgaa ttcgagctcg gtacccAAAA AAAAAATAAA
     2701 CACACATACT CATCGAGAAC TGAAAGATAT GTCTTGCCTT ATTCCTGAGA ATTTAAGGAA
     2761 CCCCAAAAAG GTTCACGAAA ATAGATTGCC TACTAGGGCT TACTACTATG ATCAGGATAT
     2821 TTTCGAATCT CTCAATGGGC CTTGGGCTTT TGCGTTGTTT GATGCACCTC TTGACGCTCC
     2881 GGATGCTAAG AATTTAGACT GGGAAACGGC AAAGAAATGG AGCACCATTT CTGTGCCATC
     2941 CCATTGGGAA CTTCAGGAAG ACTGGAAGTA CGGTAAACCA ATTTACACGA ACGTACAGTA
     3001 CCCTATCCCA ATCGACATCC CAAATCCTCC CACTGTAAAT CCTACTGGTG TTTATGCTAG
     3061 AACTTTTGAA TTAGATTCGA AATCGATTGA GTCGTTCGAG CACAGATTGA GATTTGAGGG
     3121 TGTGGACAAT TGTTACGAGC TTTATGTTAA TGGTCAATAT GTGGGTTTCA ATAAGGGGTC
     3181 CCGTAACGGG GCTGAATTTG ATATCCAAAA GTACGTTTCT GAGGGCGAAA ACTTAGTGGT
     3241 CGTCAAGGTT TTCAAGTGGT CCGATTCCAC TTATATCGAG GACCAAGATC AATGGTGGCT
     3301 CTCTGGTATT TACAGAGACG TTTCTTTACT AAAATTGCCT AAGAAGGCCC ATATTGAAGA
     3361 CGTTAGGGTC ACTACAACTT TTGTGGACTC TCAGTATCAG GATGCAGAGC TTTCTGTGAA
     3421 AGTTGATGTC CAGGGTTCTT CTTATGATCA CATCAATTTC ACACTTTACG AACCTGAAGA
     3481 TGGATCTAAA GTTTACGATG CAAGCTCTTT GTTGAACGAG GAGAATGGGA ACACGACTTT
     3541 TTCAACTAAA GAATTTATTT CCTTCTCCAC CAAAAAGAAC GAAGAAACAG CTTTCAAGAT
     3601 CAACGTCAAG GCCCCAGAAC ATTGGACCGC AGAAAATCCT ACTTTGTACA AGTACCAGTT
     3661 GGATTTAATT GGATCTGATG GCAGTGTGAT TCAATCTATT AAGCACCATG TTGGTTTCAG
     3721 ACAAGTGGAG TTGAAGGACG GTAACATTAC TGTTAATGGC AAAGACATTC TCTTTAGAGG
     3781 TGTCAACAGA CATGATCACC ATCCAAGGTT CGGTAGAGCT GTGCCATTAG ATTTTGTTGT
     3841 TAGGGACTTG ATTCTAATGA AGAAGTTTAA CATCAATGCT GTTCGTAACT CGCATTATCC
     3901 AAACCATCCT AAGGTGTATG ACCTCTTCGA TAAGCTGGGC TTCTGGGTCA TTGACGAGGC
     3961 AGATCTTGAA ACTCATGGTG TTCAAGAGCC ATTTAATCGT CATACGAACT TGGAGGCTGA
     4021 ATATCCAGAT ACTAAAAATA AACTCTACGA TGTTAATGCC CATTACTTAT CAGATAATCC
     4081 AGAGTACGAG GTCGCGTACT TAGACAGAGC TTCCCAACTT GTCCTAAGAG ATGTCAATCA
     4141 TCCTTCGATT ATTATCTGGT CCTTGGGTAA CGAAGCTTGT TATGGCAGAA ACCACAAAGC
     4201 CATGTACAAG TTAATTAAAC AATTGGATCC TACCAGACTT GTGCATTATG AGGGTGACTT
     4261 GAACGCTTTG AGTGCAGATA TCTTTAGTTT CATGTACCCA ACATTTGAAA TTATGGAAAG
     4321 GTGGAGGAAG AACCACACTG ATGAAAATGG TAAGTTTGAA AAGCCTTTGA TCTTGTGTGA
     4381 GTACGGCCAT GCAATGGGTA ACGGTCCTGG CTCTTTGAAA GAATATCAAG AGTTGTTCTA
     4441 CAAGGAGAAG TTTTACCAAG GTGGCTTTAT CTGGGAATGG GCAAATCACG GTATTGAATT
     4501 CGAAGATGTT AGTACTGCAG ATGGTAAGTT GCATAAAGCT TATGCTTATG GTGGTGACTT
     4561 TAAGGAAGAG GTTCATGACG GAGTGTTCAT CATGGATGGT TTGTGTAACA GTGAGCATAA
     4621 TCCTACTCCG GGCCTTGTAG AGTATAAGAA GGTTATTGAA CCCGTTCATA TTAAAATTGC
     4681 GCACGGATCT GTAACAATCA CAAATAAGCA CGACTTCATT ACGACAGACC ACTTATTGTT
     4741 TATCGACAAG GACACGGGAA AGACAATCGA CGTTCCATCT TTAAAGCCAG AAGAATCTGT
     4801 TACTATTCCT TCTGATACAA CTTATGTTGT TGCCGTGTTG AAAGATGATG CTGGTGTTCT
     4861 AAAGGCAGGT CATGAAATTG CCTGGGGCCA AGCTGAACTT CCATTGAAGG TACCCGATTT
     4921 TGTTACAGAG ACAGCAGAAA AAGCTGCGAA GATCAACGAC GGTAAACGTT ATGTCTCAGT
     4981 TGAATCCAGT GGATTGCATT TTATCTTGGA CAAATTGTTG GGTAAAATTG AAAGCCTAAA
     5041 GGTCAAGGGT AAGGAAATTT CCAGCAAGTT TGAGGGTTCT TCAATCACTT TCTGGAGACC
     5101 TCCAACGAAT AATGATGAAC CTAGGGACTT TAAGAACTGG AAGAAGTACA ATATTGATTT
     5161 AATGAAGCAA AACATCCATG GAGTGAGTGT CGAAAAAGGT TCTAATGGTT CTCTAGCTGT
     5221 AGTCACGGTT AACTCTCGTA TATCCCCAGT TGTATTTTAC TATGGGTTTG AGACTGTTCA
     5281 GAAGTACACG ATCTTTGCTA ACAAAATAAA CTTGAACACT TCTATGAAGC TTACTGGCGA
     5341 ATATCAGCCT CCTGATTTCC CAAGAGTTGG GTACGAATTC TGGCTAGGAG ATAGTTATGA
     5401 ATCATTTGAA TGGTTAGGTC GCGGGCCCGG CGAATCATAT CCGGATAAGA AGGAATCTCA
     5461 AAGATTCGGT CTTTACGATT CCAAAGATGT AGAGGAATTC GTATATGACT ATCCTCAAGA
     5521 AAATGGAAAT CATACAGATA CCCACTTTTT GAACATCAAA TTTGAAGGTG CAGGAAAACT
     5581 ATCGATCTTC CAAAAGGAGA AGCCATTTAA CTTCAAGATT TCAGACGAAT ACGGGGTTGA
     5641 TGAAGCTGCC CACGCTTGTG ACGTTAAAAG ATACGGCAGA CACTATCTAA GGTTGGACCA
     5701 TGCAATCCAT GGTGTTGGTA GCGAAGCATG CGGACCTGCT GTTCTGGACC AGTACAGATT
     5761 GAAAGCTCAA GATTTCAACT TTGAGTTTGA TCTCGCTTTT GAATAAGAAT TTTATACTTA
     5821 GATAAGTATG TACTTACAGG TATATTTCTA TGAGATACTG ATGTATACAT GCATGATAAT
     5881 ATTTAAACGG TTATTAGTGC CGATTGTCTT GTGCGATAAT GACGTTCCTA TCAAAGCAAT
     5941 ACACTTACCA CCTATTACAT GGGCCAAGAA AATATTTTCG AACTTGTTTA GAATATTAGC
     6001 ACAGAGTATA TGATGATATC CGTTAGATTA TGCATGATTC ATTCCTACAA CTTTTTCGTA
     6061 GCATAAGGAT TAATTACTTG GATGCCAATA AAAAAAAAAA ACATCGAGAA AATTTCAGCA
     6121 TGCTCAGAAA CAATTGCAGT GTATCAAAGT AAAAAAAAGA TTTTCACTAC ATGTTCCTTT
     6181 TGAAGAAAGA AAATCATGGA ACATTAGATT TACAAAAATT TAACCACCGC TGATTAACGA
     6241 TTAGACCGTT AAGCGCACAA CAGGTTATTA GTACAGAGAA AGCATTCTGT GGTGTTGCCC
     6301 CGGACTTTCT TTTGCGACAT AGGTAAATCG AATACCATCA TACTATCTTT TCCAATGACT
     6361 CCCTAAAGAA AGACTCTTCT TCGATGTTG   
//
