>630_A_HXT11 (24-mer)
CGTTCAAAGTATCAGTAGCAGTTC

>629_D_HXT11 (23-mer)
CATGATATCGTAGCTGCTTGAAC

>628_phor (56-mer)
tggttttttttgatgtttttcatatgcatTGGTAATCTCGAATTTGCTTGCTCTAT

>627_HygMX4_rev (67-mer)
gtggagacggggtcgttacaaaagactctattaccctgttatccctattattcctttgccctcggac

>626_ScTEF1tpr_PacI (35-mer)
taattaaTTTGTAATTAAAACTTAGATTAGATTGC

>625_ScPDC1tpr_PacI (34-mer)
taattaaTTTGATTGATTTGACTGTGTTATTTTG

>624_ScFBA1tpr_PacI (29-mer)
taattaaTTTGAATATGTATTACTTGGTT

>623_ScTDH3tpr_PacI (33-mer)
taattaaTTTGTTTGTTTATGTGTGTTTATTCG

>622_ScPGI1tpr_PacI (28-mer)
taattaaTTTTAGGCTGGTATCTTGATT

>621_ScTPI1tpr_PacI (35-mer)
taattaaTTTTAGTTTATGTATGTGTTTTTTGTAG

>620_XR_fwd (28-mer)
AAATGCCTTCTATTAAGTTGAACTCTGG

>619_XR_rev (26-mer)
TTAGACGAAGATAGGAATCTTGTCCC

>618_XDH_fwd (26-mer)
AAATGACTGCTAACCCTTCCTTGGTG

>617_XDH_rev (24-mer)
TTACTCAGGGCCGTCAATGAGACA

>616_Xk_fwd (25-mer)
AAATGTTGTGTTCAGTAATTCAGAG

>615_XK_rev (24-mer)
TTAGATGAGAGTCTTTTCCAGTTC

>614_TAL1_fwd (25-mer)
AAATGTCTGAACCAGCTCAAAAGAA

>613_TAL1_rev (26-mer)
TTAAGCGGTAACTTTCTTTTCAATCA

>612_XKS1casfw (21-mer)
acgacggccagtgaattcgag

>611_XKS1casrv (29-mer)
tgcatcattctatacgtgtcattctgaac

>610_XRcasfw (27-mer)
aacgatacctggcagtgactcctaagc

>609_XRcasrv (18-mer)
cccgtcctgtggatccgt

>608_XDHcasfw (29-mer)
gacaacacctgttgtaatcgttcttccac

>607_XDHcasrv (28-mer)
aaacagctatgaccatgattacgccaag

>606_TALcasfw (29-mer)
AGTTATTAGGTGATATCAGATCCACTAGG

>605_D-tal1 (25-mer)
GATTCATATGGGACGTTGATTTAAG

>604_phof (56-mer)
gtaattaatcagcgaagcgatgatttttgaGATCCGAAAGTTGTATTCAACAAGAA

>603_lowgc_rev
AAGTTGGAAATCTAGCTTTTCTTgacgtcagcggccgcattgcaca

>602_Lowgc_fwd
TTTCACTAGTTACTTGTAGTCGacgtgccatctgtgcagacaaacgcatcaggatat

>601_Sc_ade2_fwd
GAAAATCGGACAAAACAATCAAGTTATATAAGTTTATTGATATACTTGTACAGC

>600_Sc_ade2_rev
GCTGTACAAGTATATCAATAAACTTATATAACTTGATTGTTTTGTCCGATTTTC

>599_A_ade2
TATTAGTGAGAAGCCGAGAATTTTG

>598_D_ade2
ACTACAAAGATATCCTGACGTAGCG

>597_A_A_ade2
GATGCGGAATTGACTTTTTCTTGA

>596_D_D_ade2
TAGAAAAGGTTACACGGTACAGTC

>595_pRS306_fwd
taaagggagcccccgatttagagc

>594_pRS306_rev
tcgctttcttcccttcctttctcg

>593_GIN11_rev
gatcgtaaataatacacacatact

>592_GAL10_fwd
tcaaaaatcatcgcttcgctg

>591_ScPDC5_loxP_fd
ATCTCAAAGAGAACAACACAATACAATAACAAGAAGAACAAAATGCAGCTGAAGCTTCGTACGC

>590_PDC6-Tc1
TCAATCTTTCAAATAAGTATTTTCCAAGAGTAATTTCAGAAAGCTTCGTACGAGCGTAATC

>589_PDC6-Tc2
GCAGGCGATCGTACATCTTCCAAGCATCTCATAAACCTATGCATAGGCCACTAGTGGATCTG

>588_PDC1-Tc1
TTAATCTTTCGAACAAATATTTACCCAAAGTAATTTCAGAAAGCTTCGTACGAGCGTAATC

>587_PDC1-Tc2
AGGGTAGCCTCCCCATAACATAAACTCAATAAAATATATAGCATAGGCCACTAGTGGATCTG

>586_YlACC1_6264_F (20-mer)
CTCCTCTCTCAAGAAGCAGC

>585_3race_Adap (18-mer)
GACTCGAGTCGACATCGA

>584_B-PDC6 (25-mer)
TGGTGTTAACATTAACTTGCTTCAA

>583_B-PDC1 (25-mer)
GGTGTTAACGTTGACTTGCTTTAAT

>582_3race_Sint (36-mer)
GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTTT

>581_ScPDC5_loxP_rv (67-mer)
AAAATACACAAACGTTGAATCATGAGTTTTATGTTAATTAGCTTAGCATAGGCCACTAGTGGATCTG

>580_GXF1_YPK_fwd (83-mer)
gtcgaggaacgccaggttgcccactttctcactagtgaAAatgtcacaagattcgcattctt

>579_GXF1_YPK_rev (83-mer)
ATTTAAatcctgatgcgtttgtctgcacagatggcgcgttaaacctgttcgtcggtggcctt

>578_crp42-70 (29-mer)
gttcttgtctcattgccacattcataagt

>577_crp585-557 (29-mer)
gttctgatcctcgagcatcttaagaattc

>576_pBR322_2 (25-mer)
gaaaaataaacaaataggggttccg

>575_pBR322_1 (21-mer)
tcgttccactgagcgtcagac

>574_marker_1 (25-mer)
aaactcacgttaagggattttggtc

>573_marker_2 (29-mer)
taaatacattcaaatatgtatccgctcat

>572_ScHIS3fwd (27-mer)
CGCGCCAAAatgacagagcagaaagcc

>571_ScHIS3rev (27-mer)
CCctacataagaacacctttggtggag

>570_sso7_f (27-mer)
GATCATATGGCAACCGTAAAGTTCAAG

>569_sso7_r (28-mer)
GATCATATGCTTTTTCTGCTTCTCCAGC

>568_pCAPsAjiIR (22-mer)
GTGCcatctgtgcagacaaacg

>567_pCAPsAjiIF (23-mer)
GTcggctgcaggtcactagtgag

>566_T7_prom_F (24-mer)
GAAATTAATACGACTCACTATAGG

>565_T7_term_R (20-mer)
TATGCTAGTTATTGCTCAGC

>564_YlACC1_628_R (25-mer)
tcgtccactccggttccagaccacg

>563_pfu-rev314 (24-mer)
gatgttctctaactttttctctaa

>562_I-SceI_R (29-mer)
aacaatatgtagggataacagggtaatcc

>561_pCAPs_release_re (31-mer)
ATTTAAatcctgatgcgtttgtctgcacaga

>560_pCAPs_release_fw (25-mer)
gtcgaggaacgccaggttgcccact

>559_pCAPsAscI-5P_F (31-mer) 5'PHO
CCGGCTGCAGGTCACTAGTGAGAAAGT

>558_pCAPsAscI-5P_R (26-mer)
CGCGCCATCTGTGCAGACAAAC

>557_RPE1_YPK_fwd (29-mer)
CGCGCCAAAATGGTCAAACCAATTATAGC

>556_RPE1_YPK_rev (22-mer)
GGCTAATCTAGCAAATCTCTAG

>555_RKI1_YPK_fwd (29-mer)
CGCGCCAAAATGGCTGCCGGTGTCCCAAA

>554_RKI1_YPK_rev (26-mer)
GGTCACTTTTCGGTAACTTCAACACT

>553_GXF1_YPK_fwd (29-mer)
CGCGCCAAAatgtcacaagattcgcattc

>552_GXF1_YPK_rev (22-mer)
GGttaaacctgttcgtcggtgg

>551_GXS1_YPK_fwd (27-mer)
CGCGCCAAAatgggtttggaggacaat

>550_GXS1_YPK_rev (22-mer)
GGttaaacagaagcttcttcag

>549_ScPGI1tpf (27-mer)
ttaaatAATTCAGTTTTCTGACTGAGT

>548_ScPGI1tpr_FspAI (28-mer)
GCGCATTTTTTAGGCTGGTATCTTGATT

>547_ScTDH1tpf (27-mer)
ttaaatAAAAGCTCGACCTTAAATAAG

>546_ScTDH1tpr_FspAI (28-mer)
gcgcatTTTGTTTTGTGTGTAAATTTAG

>545_ScRPL11btpf (26-mer)
ttaaatCTTGGACGCGTATTTTTGTA

>544_ScRPL11btpr_FspA (25-mer)
GCGCATTCAAAATAAATCAACAATC

>543_ScRPL12Atpf (26-mer)
ttaaatTTTTGTATATCCTTTGTTCT

>542_ScRPL12Atpr_FspA (26-mer)
gcgcatTCTGGAATTTACCCTAACTG

>541_ScRPS18btpf (27-mer)
ttaaatCTTTACCACTTTCTGCTCTAT

>540_ScRPS18btpr_FspA (26-mer)
gcgcatTTTTCATATGTACACATACA

>539_ScRPS19atpf (26-mer)
ttaaatTTTTACTCTATTTGTCGATC

>538_ScRPS19atpr_FspA (26-mer)
gcgcatGTTAACTGAAATGAAAATTT

>537_ScRPS19btpf (27-mer)
ttaaatCTTTATTATCTTTGGTTCTAT

>536_ScRPS19btpr_FspA (26-mer)
gcgcatTCTAGTATGGTTTGAAACCT

>535_pSU0_2u_Fwd (21-mer)
taactgtcagaccaagtttac

>534_pSU0_2u_rev (27-mer)
ttatcgatgataagctgtcaaagatga

>533_A-Gal2 (25-mer)
tggcattatactcctgctagaaagt

>532_D-Gal2 (25-mer)
tctggtgatgtggtcctttaataat

>531_pSU1_MX4f (67-mer)
taattctcatctttgacagcttatcatcgataagtccccgccgggtc

>530_ScZWF1_YPK_rv (28-mer)
CCctaattatccttcgtatcttctggct

>529_ScZWF1_YPK_fw (28-mer)
CGCGCCAAAatgagtgaaggccccgtca

>528_ScALD6_YPK_rv (28-mer)
CCttacaacttaattctgacagctttta

>527_ScALD6_YPK_fw (29-mer)
CGCGCCAAAatgactaagctacactttga

>526_ScACS1_YPK_rv (28-mer)
CCttacaacttgaccgaatcaattagat

>525_ScACS1_YPK_fw (28-mer)
CGCGCCAAAatgtcgccctctgccgtac

>524_pFA6aF (29-mer)
cacatacgatttaggtgacactatagaac

>523_AgTEF1tpR (21-mer)
ggttgtttatgttcggatgtg

>522_AgMUD1tpF (27-mer)
tcagtactgacaataaaaagattcttg

>521_pFA6aR (20-mer)
cgactcactatagggagacc

>520_KlLEU2ttF (20-mer)
agagtcttttgtaacgaccc

>519_KlNFS1ttF (22-mer)
gctggaacaaaagtgatctata

>518_crp252f (27-mer)
ctgtttgaagagggccaggaacgtagc

>517_crp459r (25-mer)
ccgggtgagtcatagcgtctggttg

>516_T4lig_fwd (28-mer)
gatCATATGATTCTTAAAATTCTGAACG

>515_T4lig_rev (29-mer)
gatGGATCCTAGACCAGTTACCTCATGAA

>514_pTACf (21-mer)
TGAAATGAGCTGTTGACAATT

>513_GFP_orfF (29-mer)
ATGTATAAAAAATCTAACAACCCGTTTTC

>512_crp_EcoRV (29-mer)
ttcgccaattgattcaggtaaatccggat

>511_crp_ZraI (29-mer)
gtcagcggccgcattgcacagactctgct

>510_mkk1_rev (20-mer)
ACCAGTGCCGCCCACAGTAA

>509_mycGFPr (20-mer)
CTACTTGTACAGCTCGTCCA

>508_TDHpromFWD
ACAAGGCAATTGACCCACGCATGTATCTA

>507_D-pdc5
CTAAAGGTACAAAACCGAATACGAA

>506_A-pdc5
AAAAATTGATTCTCATCGTAAATGC

>505_YlACC1r_CpoI
TCCGtcacaaccccttgagcagctca

>504_YlACC1f_SgsI
CCAAatgcgactgcaattgaggacact

>503_MX4bl-PI-SceIr
TCCGATCTATGTCGGGTGCGGAGAAAGAGGTAATcaagaatctttttattgtcagtactgatta

>502_MX4bl-I-SceIr
TCCGATTACCCTGTTATCCCTAcaagaatctttttattgtcagtactgatta

>501_MX4blf
CCAAtcacatcacatccgaacataaacaaccatg

>500_pCAPsEcoRV5Pr
ATCCGGATTTACCTGAATCAATTGGCGAAATTTTTTGTACG

>499_pCAPsEcoRV5Pf
ATCCTGATGCGTTTGTCTGCACAGATGGCG

>498_pCAPsZraI5Pr
GTCGAGGAACGCCAGGTTGCCCACTTTC

>497_pCAPsZraI5Pf
GTCAGCGGCCGCATTGCACAGACT

>496_HS1 (27-mer)
GATCATATGGCCCTGGAGGAGGCCCCC

>495_HS2 (26-mer)
GAAGCTTACTCCTTGGCGGAGAGCCA

>494_tp_Eco32I_rev2 (29-mer)
ttcgccaattgattcaggtaaatccggat

>493_pCAPsAscI5PF (5'P)
CCGGCTGCAGGTCACTAGTGAG

>492_pCAPsAscI5PR (5'P)
CGCGCCATCTGTGCAGACAAAC

>491_A-pox1 (25-mer)
TATTAGTTGATTAAACTCCGAAGCG

>490_D-pox1 (25-mer)
AAACACTCAATAATTACCTGCCATC

>489_D1tpf_SmiI (26-mer)
TTAAATCTACGAGACCGACACCGTAA

>488_U1tpr_FspAI (34-mer)
GCGCATCTATTCAAGAGACCTCGTGGACATCCAT

>487_PrimerFwdCL2b (26-mer)
atgtcagcgaaatccattcacgaggc

>486_PrimerRevCL2b (25-mer)
ttaaactccgagaggagtggaagcc

>485_KlLEU2fwd-c2 (61-mer)
tacggttcctggccttttgctggccttttgctcacatgttAAGATCCCAGCAAAGGCTTAC

>484_yeast_DNA_int_f
GTGAATGAATACATGAAAGATTCAT

>483_yeast_DNA_int_r
CTTTTCTTCCTTCAATGATTTCC

>482_PrimerFwdACLB1 (32-mer)
AGGCGCGCCAAAatggctacgggacagctatt

>481_PrimerRevACLB1 (33-mer)
AGGCGCGCCttacttggtgtacaagacatcttc

>480_PrimerFwdACBL2 (32-mer)
AGGCGCGCCAAAatggcaacgggacagctttt

>479_PrimerRevACBL2 (33-mer)
AGGCGCGCCttacttggtgtacaacacatcttc

>478_CEN6ARSHf (58-mer)
TTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAAacggatcgcttgcctgta

>477_CEN6ARSHr (30-mer)
cttttcatcacgtgctataaaaataattat

>476_bleMX4f (58-mer)
AATTTAAATTATAATTATTTTTATAGCACGTGATGAAAAGgtccccgccgggtcaccc

>475_bleMX4r (59-mer)
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGgagctcgttttcgacactg

>474_MSW_fwd (18-mer)
TCCTTGACAGTCTTGACG

>473_MSW_rev (18-mer)
GTATAGCGACCAGCATTC

>472_Kanfwd2 (29-mer)
gcgataatgtcgggcaatcaggtgcgaca

>471_Kanrev (29-mer)
aaaactcaccgaggcagttccataggatg

>470_pSU0f-dEcoRV (50-mer)
gtttactaaaaacacatgtggatattttgactgatttttccatggagggc

>469_pSU0r-dEcoRV (50-mer)
gccctccatggaaaaatcagtcaaaatatccacatgtgtttttagtaaac

>468_pCAPs_release_fw (25-mer) 79.66
gtcgaggaacgccaggttgcccact

>467_pCAPs_release_re (31-mer)
ATTTAAatcctgatgcgtttgtctgcacaga

>466_tp_ZraI_fwd (31-mer) this works well according to FP
gagtctgtgcaatgcggccgctgacTTAAAT

>465_tp_ZraI_rev (31-mer)
agtgggcaacctggcgttcctcgacGCGCAT

>464_tp_Eco32I_fwd (31-mer)
tctgtgcagacaaacgcatcaggatTTAAAT

>463_tp_Eco32I_rev (31-mer) this primer does not work so well according to FP
ccaattgattcaggtaaatccggatGCGCAT

>462_Primer_fwd_1b (29-mer)
GATCTGTACATGCAGCTCTCGGAGGGCGA

>461_Primer_rev_1 (29-mer)
TACATACCTCGCTCTGCTAATCCTGTTAC

>460_Primer_rev_2b (29-mer)
GATCTGTACAGGCCGCGCTTCTGCGGGCG

>459_Primer_fwd_2 (29-mer)
GGTAAGACACGACTTATCGCCACTGGCAG

>458_nat_fwd (29-mer)
GATCTGTACAAGCTTGCCTTGTCCCCGCC

>457_nat_rev (29-mer)
GATCTGTACATCGACACTGGATGGCGGCG

>456_ScTPI1tpr_FspAI (36-mer)
GCGCATTTTTTAGTTTATGTATGTGTTTTTTGTAGT

>455_ScTEF1tpr_FspAI (36-mer)
GCGCATTTTTGTAATTAAAACTTAGATTAGATTGCT

>454_ScTDH3tpr_FspAI (33-mer)
GCGCATTTTTGTTTGTTTATGTGTGTTTATTCG

>453_ScPDC1tpr_FspAI (31-mer)
GCGCATTTTTGATTGATTTGACTGTGTTATT

>452_ScENO2tpr_FspAI (39-mer)
GCGCATTTATTATTGTATGTTATAGTATTAGTTGCTTGG

>451_ScFBA1tpr_FspAI (34-mer)
GCGCATTTTTGAATATGTATTACTTGGTTATGGT

>450_PDC5-Tc1_61bp (61-mer)
TCTGAAATAACCTTAGGTAAATATTTATTTGAAAGATTGAaagcttcgtacgagcgtaat
c

>449_PDC5-Tc2_62bp (62-mer)
TTGCTAAAAGAACTTGTATATAAGAGCTGCCATTCTCGATgcataggccactagtggatc
tg

>448_PrimerFwdCL1 (35-mer)
GATAGGCGCGCCAAAATGTCTGCCAACGAGAACAT

>447_PrimerRevCL1 (32-mer)
GATAGGCGCGCCCTATGATCGAGTCTTGGCCT

>446_PrimerFwdCL2 (35-mer)
GATAGGCGCGCCAAAATGTCAGCGAAATCCATTCA

>445_PrimerRevCL2 (32-mer)
GATAGGCGCGCCTTAAACTCCGAGAGGAGTGG

>444_A-PDC1 (25-mer)
CAAATATCGTTTGAATATTTTTCCG

>443_D-PDC1 (25-mer)
TACACTAATGCAGTTTCAGGGTTTT

>442_A-PDC6 (25-mer)
AGAACGAATACTTTTTAAGCCGAAT

>441_D-PDC6 (25-mer)
AAAAATAATAATTGACGTGGCTGAA

>440_ScPDC5rev (23-mer)
GTGGTTTCAGAAATGTTGGCAGA

>439_pSH47_Prom_fw (58-mer)
ATTCGATACTAACGCCGCCATCCAGTGTCGAAAACtcactaaagggaacaaaagctgg

>438_pSH47_Term_rv (60-mer)
CATACATTATACGAAGTTATATTAAGGGTTCTCGAtaatacgactcactatagggcgaat

>437_D-aco1
TGACAGACGGTGCACTTATTACTTA

>436_C-aco1
CTGCTAGAGATTACAGAGACCAAGG

>435_B-aco1
GGTAAATGGCCTGTTTAATCTTTTT

>434_A-aco1
AAAATTTGAACCCTTCAGTTGTTCT

>433_Cit1_rev
ctcgagttagttcttactttcgattt

>432_Cit1_fwd
ggatccatgtcagcgatattatcaac

>431_MDH3RevΔSKL
ctcgagtcaagagtctaggatgaaactc

>430_mdh3_rev
ctcgagtcatagcttggaagagtcta

>429_mdh3_fwd
ggatccatggtcaaagtcgcaattct

>428_YPK-GFPS65Tf (60-mer)
GTTTAAAATCCTGATGCGTTTGTCTGCACAGATGGatgagtaaaggagaagaacttttca

>427_YPK-GFPS65Tr (63-mer)
AATTTCCTGACGGGTAATTTTGATTTGCATGCCGTCCGctatttgtatagttcatccatg
cca

>426_ScPDC1_loxP_fwd (64-mer)
ACTCATAACCTCACGCAAAATAACACAGTCAAATCAATCAAAATGcagctgaagcttcgt
acgc

>425_ScPDC1_loxP_rev (67-mer)
AATGCTTATAAAACTTTAACTAATAATTAGAGATTAAATCGCTTAgcataggccactagt
ggatctg

>424_ScPDC6_loxP_fwd (64-mer)
TAAAAAACCCACGTAATATAGCAAAAACATATTGCCAACAAAATGcagctgaagcttcgt
acgc

>423_ScPDC6_loxP_rev (67-mer)
TTTATTTGCAACAATAATTCGTTTGAGTACACTACTAATGGCTTAgcataggccactagt
ggatctg

>422_CORE_FAA1_I-Sce (81-mer)
TAGTATGATGAGGCTTTCCTATCATGGAAATGTTGATCCATAATGGGACAATAGGGATTT
ATTCGTACGCTGCAGGTCGAC

>421_CaART1_1221_F (20-mer)
gctcctgaagttttcccatt

>420_CaART1_132_R (20-mer)
tggcagaaattgatgaaaca

>419_ScTPI1tpf (32-mer)
TTAAATTGTTTAAAGATTACGGATATTTAACT

>418_ScTPI1tpr (37-mer)
TTTAAACTTTTTAGTTTATGTATGTGTTTTTTGTAGT

>417_ScTEF1tpf (30-mer)
TTAAATAACAATGCATACTTTGTACGTTCA

>416_ScTEF1tpr (37-mer)
TTTAAACTTTTGTAATTAAAACTTAGATTAGATTGCT

>415_ScTDH3tpf (29-mer)
TTAAATAATAAAAAACACGCTTTTTCAGT

>414_ScTDH3tpr (34-mer)
TTTAAACTTTTGTTTGTTTATGTGTGTTTATTCG

>413_ScPDC1tpf (24-mer)
TTAAATAAGGGTAGCCTCCCCATA

>412_ScPDC1tpr (32-mer)
TTTAAACTTTTGATTGATTTGACTGTGTTATT

>411_ScENO2tpf (23-mer)
TTAAATACGCGGCGTTATGTCAC

>410_ScENO2tpr (40-mer)
TTTAAACTTATTATTGTATGTTATAGTATTAGTTGCTTGG

>409_ScFBA1tpf (37-mer)
TTAAATAATAACAATACTGACAGTACTAAATAATTGC

>408_ScFBA1tpr (35-mer)
TTTAAACTTTTGAATATGTATTACTTGGTTATGGT

>407_pTACf (21-mer)
TGAAATGAGCTGTTGACAATT

>406_mycGFPr (20-mer)
CTACTTGTACAGCTCGTCCA

>405_KlLEU2fwd-c (60-mer)
gtcaggggggcggagcctatggaaaaacgccagcaacgcggctgtgaagatcccagcaaa

>404_KlLEU2rev-c (57-mer)
atgaccccaggtgtaggtGGATCCcgatAAGCTTaattgggttaaccttgtttgctg

>403_KlURA3fwd-c (68-mer)
tgcatttttgttctacaaaatgaagcacagatgcttcgttgttttatttaggttctatcg
aggagaaa

>402_KlURA3rev-c (60-mer)
ccagcaaacaaggttaacccaattAAGCTTatcgGGATCCacctacacctggggtcatga

>401_HIS1_fw (21-mer)
tcatcctccaggtaccggatc

>400_RPS10_rv (27-mer)
aaattgagagttgatgaagtccaaggt

>399_RPS10_fw (30-mer)
attgccattttctgaccaatgatcttaatt

>398_JEN2loc_rv (29-mer)
ttgaactgtacaactaattgttagtgctc

>397_CYC1termrev2 (29-mer)
AATGTAAGCGTGACATAACTAATTACATG

>396_ScFAA4f (50-mer)
AAACTAAGAAGTACGCATCAAAATGTAAAGGAAGACATAGTTTTTTACTT

>395_ScFAA4r (50-mer)
AAGTAAAAAACTATGTCTTCCTTTACATTTTGATGCGTACTTCTTAGTTT

>394_kanBeta (18-mer)
GTCGACCTGCAGCGTACG

>393_kanGamma (24-mer)
GAAAACGAGCTCGAATTCATCGAT

>392_PROMrecPsXYL1f (55-mer)
TCTAGAACTAGTGGATCCCCCGGGCTGCAGAAAatgccttctattaagttgaact

>391_CYC1t_recPsXYL1r (53-mer)
CGACGGTATCGATAAGCTTGATATCGAATTCttagacgaagataggaatcttg

>390_T7term(19-mer)
GCTAGTTATTGCTCAGCGG

>389_AgTEF1p_rev(25-mer)
ggttgtttatgttcggatgtgatgt

>388_AgTEF1t_fwd (27-mer)
tcagtactgacaataaaaagattcttg

>387_A-PGI1 (2-mer)
GTGTGGGTGTATTGGATTATAGGAA

>386_B-PGI1 (25-mer)
GTGATCTTCTTACCGGTATAACCCT

>385_C-PGI1 (25-mer)
TCTACCAACGGTTTAATCAATCAAT

>384_D-PGI1 (25-mer)
GGTATACTGGAGGCTTCATGAGTTA

>383_up-PGI1 (64-mer)
AGTCTTGCAAAATCGATTTAGAATCAAGATACCAGCCTAAAAATGCAGCTGAAGCTTCGT
ACGC

>382_dn-PGI1 (67-mer)
TAGCTTTAATGTTCTTTAGGTATATATTTAAGAGCGATTTGTTCAgcataggccactagt
ggatctg

>381_cip20_10122_rev (22-mer)
acgccaagcgcgcaattaaccc

>380_cip20_3551_fwd (20-mer)
ttttcaatttcacggccaat

>379_2my_4057_rev (18-mer)
GAACCGGGGATGCGACgt

>378_2my_4936_fwd (27-mer)
GCACAGAGATATATAGCAAAGAGATAC

>377_cip20_5676_rev (24-mer)
caacagatctaccggtttaaagaa

>376_N1 (50-mer)
CCTCGCTGCCCAGCCGGCGATGGCCtataaaaaatctaacaacccgtttt

>375_N2 (50-mer)
aaaacgggttgttagattttttataGGCCATCGCCGGCTGGGCAGCGAGG

>374_N3 (50-mer)
tatctcctccgaaactttcctgaaaCTCGAGATCAAACGGGCTAGCCAGC

>373_N4 (50-mer)
GCTGGCTAGCCCGTTTGATCTCGAGtttcaggaaagtttcggaggagata

>372_ScFAA1 (50-mer)
AAAGACAAAAAAAGACAACAATATGTAATGGATCAACATTTCCATGATAG

>371_ScFAA1 (50-mer)
CTATCATGGAAATGTTGATCCATTACATATTGTTGTCTTTTTTTGTCTTT

>370_ScGAL2_int_fw (20-mer)
GGCATCGCCGTCTTATGTCC

>369_ScGAL2_int_rv (20-mer)
CACCAACAGAGGCGTAGATG

>368_Pg3012ART1_int_f (20-mer)
TCCCTTGGGGTGTCCAGATT

>367_Pg3012ART1_int_r (19-mer)
CAGCGGCTCCGAACAACAA

>366_ALD6-C
GTAAGAAGGTTGGTATTCCAGCT

>365_B-Ald6
AGCAGCATCTCTTAGACAGTTGATT

>364_ACS1-C
GAGCTGGTGATTCCTACATCGAA

>363_B-Acs1
GACCAGTTTAAAAATTGGGTAGCTT

>362_D-snf2
CAAGAACAAGTTCACTATGATGACG

>361_A-snf2
TTAATTCCAATACATTCGCCACTAT

>360_D-faa4
GAAATCAAGAATGAAGCAAGACATT

>359_A-faa4
TCGTTCATCTCGTTCTTTCTACTCT

>358_D-faa1
GGTAATGCTTCTTATGTGAGGTGTC

>357_A-faa1
ATGATACAGGCACGAATACATCATA

>356_CaART1_rev (23-mer)
ttaactatctaaagaccgaacgg

>355_Pg3012ART1_rev (22-mer)
TTAGACAGTTGGCTTTTCCTCC

>354_ScGAL2_rev (22-mer)
TTATTCTAGCATGGCCTTGTAC

>353_TDHpromFWD3 (29-mer)
ACAAGGCAATTGACCCACGCATGTATCTA

>352_CYC1t_Pg05324_re (50-mer)
TGACTCGAGGTCGACGGTATCGATAAGCTTGctactcaacgtgcgacacc

>351_PromrecPg05324_F (50-mer)
CCCGGGCTGCAGGAATTCGATATCAAGCTTatgacagaaccaacaaatcc

>350_GFPS65T_Fwd (20-mer)
GTCAGTGGAGAGGGTGAAGG

>349_A-faa1 (25-mer)
ATGATACAGGCACGAATACATCATA

>348_D-faa1 (25-mer)
GGTAATGCTTCTTATGTGAGGTGTC

>347_A-faa4 (25-mer)
TCGTTCATCTCGTTCTTTCTACTCT

>346_D-faa4 (25-mer)
GAAATCAAGAATGAAGCAAGACATT

>345_A-snf2 (25-mer)
TTAATTCCAATACATTCGCCACTAT

>344_D-snf2 (25-mer)
CAAGAACAAGTTCACTATGATGACG

>343_KMR (17-mer)
TTCCCGTTGAATATGGC

>342_ORIR-1 (21-mer)
CCTTTTTACGGTTCCTGGCCT

>341_ARB2 (20-mer)
GGCCACGCGTCGACTAGTAC

>340_lacZ-1 (20-mer)
GGCGATTAAGTTGGGTAACG

>339_TN1 (24-mer)
GACATCATAACGGTTCTGGCAAAT

>338_lacZ-2 (19-mer)
TTTTCCCAGTCACGACGTT

>337_TN2 (25-mer)
TGAGCTGTTGACAATTAATCATCGG

>336_ARB1 (35-mer)
GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT

>335_MumMX4_KpnI_fw (68-mer)
gcttgagaaggttttgggacgctcgaaggctttaatttgcggccggtaccCGTACGCTGC
AGGTCGAC

>334_MumMX4_KpnI_rv (68-mer)
cagtgagcgcgcgtaatacgactcactatagggcgaattgggtaccGCATAGGCCACTAG
TGGATCTG

>333_ScCYC1term_EcoRI_fw (29-mer)
ctgcaggaattcgatatcaagcttatcga

>332_ScCYC1term_KpnI_rv (26-mer) This primer can be used as reverse primers to the ScCYC1 terminator
ggtaccggccgcaaattaaagccttc

>331_CORE-B1 (25-mer)
aggctaagataatggggctctttac

>330_CORE-B (25-mer)
gtcacccgctcggcggcttctaatc

>329_CORE-C (25-mer)
actggtatatgattttgtggacatg

>328_CORE-C3 (25-mer)
ctgtttcctgtgtgaaattgttatc

>327_Kanfwd (21-mer)
GTCGGGCAATCAGGTGCGACA

>326_Kanrev (23-mer)
CCGAGGCAGTTCCATAGGATGGC

>325_Hygfwd (27-mer)
GATGTAGGAGGGCGTGGATATGTCCTG

>324_Hygrev (27-mer)
TGCATCATCGAAATTGCCGTCAACCAA

>323_ScGAL2GFP_fw (72-mer)
AGGATTTACAACATGACGACAAACCGTGGTACAAGGCCATGCTAGAATAGTAAAGGAGAAGAACTTTTCACT

>322_MumScACS1fw (50-mer)
TCTAGAACTAGTGGATCCCCCGGGCTGCAGAATGTCGCCCTCTGCCGTAC

>321_MumScACS1rv (50-mer)
CGACGGTATCGATAAGCTTGATATCGAATTCTTACAACTTGACCGAATCA

>320_MumScALD6fw (50-mer)
TCTAGAACTAGTGGATCCCCCGGGCTGCAGAATGACTAAGCTACACTTTG

>319_MumScALD6rv (50-mer)
CGACGGTATCGATAAGCTTGATATCGAATTCTTACAACTTAATTCTGACA

>318_GFP_rev-2 (25-mer)
gcatcaccttcaccctctccactga

>317_CYC1termrev2 (29-mer)
AATGTAAGCGTGACATAACTAATTACATG

>316_CORE_FAA1_fwd (78-mer)
ACTAGAACAAACACAAAAGACAAAAAAAGACAACAATATGTAGGGATAACAGGGTAATCC
GCGCGTTGGCCGATTCAT

>315_CORE_FAA1_rev (63-mer)
TAGTATGATGAGGCTTTCCTATCATGGAAATGTTGATCCATTATTCGTACGCTGCAGGTC
GAC

>314_CORE_FAA4_fwd (78-mer)
TCACTATTTCTTGAAAAACTAAGAAGTACGCATCAAAATGTAGGGATAACAGGGTAATCC
GCGCGTTGGCCGATTCAT

>313_CORE_FAA4_rev (63-mer)
TATGAAGGGCAGGGGGGAAAGTAAAAAACTATGTCTTCCTTTATTCGTACGCTGCAGGTC
GAC

>312_CORE_SNF2_fwd (78-mer)
TTCTGCTATTTTCACGACTTTCGATTAATTATCTGCCATGTAGGGATAACAGGGTAATCC
GCGCGTTGGCCGATTCAT

>311_CORE_SNF2_rev (63-mer)
CGTATAAACGAATAAGTACTTATATTGCTTTAGGAAGGTACTATTCGTACGCTGCAGGTC
GAC

>310_A-STE20 (25-mer)
CGTAGAAGACGATAGAACGAAAAAG

>309_B-STE20 (25-mer)
GTTATCAAGACTGTCCTTGTCTGGT

>308_C-STE20 (25-mer)
TAGATATCTGGTCGTTGGGTATCAT

>307_D-STE20 (25-mer)
CCAAAATTGGATAATACCTCTCCTT

>306_A-SSK1 (25-mer)
TTTGGTATCCCTTAACCTAATTTCC

>305_B-SSK1 (25-mer)
TCATCAGTGGAGTTGTCTATTCGTA

>304_C-SSK1 (25-mer)
CGACTACCTGACTAAACCAGTGAAT

>303_D-SSK1 (25-mer)
TTCATCCATGAAATCCTTCATAACT

>302_A-STE2 (25-mer)
CTCGTGCATTAAGACAGGCTAGTAT

>301_B-STE2 (25-mer)
TCAAACCTATCCTTTTGAAGTTGTC

>300_C-STE2 (25-mer)
AACATCCACAGATAGGTTTTATCCA

>299_D-STE2 (25-mer)
GAGTTCTAGATCATGGCAAGAAAAA

>298_A-STE3 (25-mer)
GGAAACTTCTCGACTATCAATGTGT

>297_B-STE3 (25-mer)
ACAGTACTAAGGTGGCATAAACAGC

>296_C-STE3 (25-mer)
AGCACCATCTGGAATACCATTATTA

>295_D-STE3 (25-mer)
TTTTTCTTGTGTGGAACTTGTATCA

>294_A-MID2 (25-mer)
TTCAAGGCTTTCAAATTAAGTTACG

>293_B-MID2 (25-mer)
TGATAGTGCTTCCTTGATTATAGGC

>292_C-MID2 (25-mer)
GCCTATAATCAAGGAAGCACTATCA

>291_D-MID2 (25-mer)
GTTAAAGCACTCGAAACCTTATTCA

>290_A-SHO1 (25-mer)
ACGTACATAGACCCTTGAACCTACA

>289_B-SHO1 (25-mer)
GAGGAGTTGGTCTTATCTTTGATGA

>288_C-SHO1 (25-mer)
GGTTTTGAGAATACTCAGCCAAATA

>287_D-SHO1 (24-mer)
TTCCAACTCTGGTGTTGTTGTTTA

>286_A-SLG1 (25-mer)
ATTTAACGCAGTGTAGTTTTCTTGG

>285_B-SLG1 (25-mer)
AGTCAAGTTGGTACACAGAATAGGC

>284_C-SLG1 (25-mer)
TACGCCTCTTCATTTTCATCTAATC

>283_D-SLG1 (27-mer)
TTTTATTAGGTGGTATTCATTTCAAAG

>282_A-WsC2 (25-mer)
AGAAATAAAGAGGAGAAACCCTCAA

>281_B-WsC2 (25-mer)
AGAAGAGGAAGAGGATGAAGAAGAG

>280_C-WsC2 (25-mer)
GTGATGCACATAATATCAGCAAAAG

>279_D-WsC2 (25-mer)
ATTGCCTCAGATTTGTGAAGAATAG

>278_A-WSC3 (25-mer)
GTAAGTGGTATACTGCAGACCAGGA

>277_B-WSC3 (25-mer)
GTAGAAACTGTCGTGGCTATCATTT

>276_C-WSC3 (25-mer)
CTTTGAATAGTGCAAATGAAAGGTT

>275_D-WSC3 (25-mer)
TATTAATTTATCTTGCTTGGTTCGC

>274_A-RVS161 (25-mer)
ACTTTTAGGTTAGCGGAGAAGATGT

>273_B-RVS161 (25-mer)
CTTTGAAATACGTCGAAAACTTTGT

>272_C-RVS161 (25-mer)
ACAAAGTTTTCGACGTATTTCAAAG

>271_D-RVS161 (25-mer)
CGTATCTTATTCCTCGCTTCTATTG

>270_A-GPD1 (25-mer)
CTTTTTCCAGTTTTCCCTATTTTGT

>269_B-GPD1 (25-mer)
ATGATTTCAGTCAATTTTTCACCAT

>268_C-GPD1 (25-mer)
CTCAAGGTTTAATTACCTGCAAAGA

>267_D-GPD1 (25-mer)
GAAGGTGTGGCACTTTGTTTATTAT

>266_A-GPD2 (25-mer)
CTCTGCCATTGTTATATTACGCTTT

>265_B-GPD2 (25-mer)
GTTACCAGAACCAATCACTGTAACC

>264_C-GPD2 (25-mer)
GGCTTACCAACTACCAAAGGATTAT

>263_D-GPD2 (25-mer)
GAGAATTACTGCAGTGAAAAAGCTC

>262_A-SLN1 (25-mer)
TTCTACCTTCATGTACTTAAACGCC

>261_B-SLN1 (25-mer)
TCTTCTGGAATTAGATCACCAGTTC

>260_C-SLN1 (25-mer)
AAGACGACGATAAAAATGAAACAAG

>259_D-SLN1 (25-mer)
GTACAACTGTGACAAATCTGTTTGC

>258_B-PKC1 (25-mer)
GATTTGGTAGAAAGAAACCCGTATT

>257_C-PKC1 (25-mer)
GGAGATGACGAAGATGAAGTTTTTA

>256_kanB (20-mer)
CTGCAGCGAGGAGCCGTAAT

>255_kanC (22-mer)
TGATTTTGATGACGAGCGTAAT

>254_kanB1 (20-mer)
TGTACGGGCGACAGTCACAT

>253_kanC3 (22-mer)
CCTCGACATCATCTGCCCAGAT

>252_NP1 (23-mer)
GTTGGCCATGAGAAATTCCATCG

>251_CaART1_23_F (20-mer)
ggttttcggtaacaggcaaa

>250_CaART1_216_F (23-mer)
ggtggttcattaattggatcatt

>249_CaART1_728_F (24-mer)
tggaaattagagaagctgttgaaa

>248_CaART1_571_R (21-mer)
tcatttgtaaaccccatggaa

>247_CaART1_1099_R (23-mer)
ccaacttcatcaacatattcacc

>246_CaART1_1563_R (21-mer)
ttcaacatgatcagcagatgg

>245_CaART1opt_38_F (21-mer)
GGCAGATGCCAAAATTCTACA

>244_CaART1opt_239_F (21-mer)
TGTCAGGTCAAGTCTGCGATA

>243_CaART1opt_716_F (23-mer)
TGCCGAGATAATGGAAATTAGAG

>242_CaART1opt_590_R (20-mer)
CACCAATAGCACAGCTGGAA

>241_CaART1opt_1100_R (20-mer)
ACCTCCGACTTCATCGACAT

>240_CaART1opt_1537_R (20-mer)
TCGTCTTCATGCACCAACTC

>239_CYC1promfwd4 (35-mer)
CTTTGCAGCATAAATTACTATACTTCTATAGACAC

>238_CYC1t_recCaART1 (62-mer)
CTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCttaactatctaaagaccgaac
gg

>237_CYC1t_recPg3012A (61-mer) stock 44uM!
CTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCTTAGACAGTTGGCTTTTCCTC
C

>236_CYC1t_recScGAL2 (61-mer)
CTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCTTATTCTAGCATGGCCTTGTA
C

>235_I-SceIfwd_PscI (65-mer)
GATCACATGTATAAAAAATCTAACAACCCGTTTTCTGATATGAAAAACATCAAAAAAAAC
CAGGT

>234_I-SceIrev_BglII (32-mer)
GATCAGATCTTTCAGGAAAGTTTCGGAGGAGA

>233_A-CIN5 (25-mer)
TTGGCATTAAATCTATGAGGAAGAG

>232_D-CIN5 (25-mer)
TCTTTCCTTTTTAGTAATGTGCCTG

>231_A-SRO7 (25-mer)
CCTTAACAGCGGATAAAATAAAGGT

>230_D-SRO7 (25-mer)
GTAAATTACGTGTCAAAAGATTGGG

>229_A-PYC2 (25-mer)
CAAAGGCACATATGACTAACCTTTT

>228_D-PYC2 (25-mer)
GAAGAAAACCTACAGTCGATCAAAA

>227_PYC2HA_fwd (54-mer)
GAAGAAGAAACCCTACCCCCATCCCAAAAAAAGTCCCACCACCATCATCATCAC

>226_PYC2HA_rev (56-mer)
GATGTCATAAAATATAATTAACGAGTAAAAAACTACTATAGGGAGACCGGCAGATC

>225_CIN5HA_fwd (51-mer)
ATTGAATCATTAAAGTCGAAATTAAAAGAATCCCACCACCATCATCATCAC

>224_CIN5HA_rev (51-mer)
GTTACTAGTACTAATAATTATTCATTATTACTATAGGGAGACCGGCAGATC

>223_SRO7HA_fwd (51-mer)
AAAGATGTAATGAAAGGTGCCCTTGGTTTTTCCCACCACCATCATCATCAC

>222_SRO7HA_rev (55-mer)
CTCATTACCCTGTATGAATTAGTGTATGTAACTACTATAGGGAGACCGGCAGATC

>221_PROMrecCaART1 (51-mer)
TCTAGAACTAGTGGATCCCCCGGGCTGCAGAatggttttcggtaacaggca

>220_PROMrecPg3012ART (55-mer)
TCTAGAACTAGTGGATCCCCCGGGCTGCAGAatgggttacgaagacaaattagtg

>219_PROMrecScGAL2 (51-mer)
TCTAGAACTAGTGGATCCCCCGGGCTGCAGAatggcagttgaggagaacaa

>218_A-PKC1 (25-mer)
ATCGAAAGTAATACGACAGGTTCAG

>217_D-PKC1 (25-mer)
TAATCCTGAGCTGTTTCTTAGATGC

>216_I-SceIrev_BglII (29-mer)
GATCAGATCTTTCAGGAAAGTTTCGGAGG

>215_I-SceIfwd_PscI (63-mer)
GATCACATGTATAAAAAATCTAACAACCCGTTTTCTGATATGAAAAACATCAAAAAAAAC
CAG

>214_ATCA_06R
nnn

>213_ATCA_05F
nnn

>212_ART1_FOR2 CaART1 fwd primer for cloning (with NdeI (and BglII) restriction site)
ATAGCAGATCTCATATGGTTTTCGGTAACAGGCAAAT

>211_ART1_REV3 ART1 PrimerREV for cloning (with HindIII restriction site)
ACTGTATAAGCTTTTAACTATCTAAAGACCGAACGGA

>210_ATCA03_REV    ART1 primerREV pos 295C
CTGAACCAATAATCCAAAATCCAC

>209_ATPGb03_FOR put PgART1b PrimerFOR
AGATCTCATATGGGTTACGAAGACAAATTAGT

>208_ATPGb04_REV2 put PgART1b PrimerREV
TCTAGAAGCTTAGACAGTTGGCTTTTCCTCCGAT

>207_GPDp_01F p426 GPD (Pair)
TCTTACACCTTCTATTACCTTCTG

>206_TEFp_01F p426 TEF (Pair)
GATGACCTCCCATTGATATTTAAG

>205_CYCt_02R p426 GPD (Pair) or p426 TEF (Pair)
CAGTATAATGTTACATGCGTACAC

>204_kanB1 primer for checking deletion EUROSCARF deletion strains
TGTACGGGCGACAGTCACAT

>203_T7_CIN5_fwd (69-mer)
CATATGGCCATGGAGGCCAGTGAATTCCACCCGGGTGGGCATCGAATGTTAATGCAAATA
AAAATGGAC

>202_T7_SRO7_fwd (63-mer)
CATATGGCCATGGAGGCCAGTGAATTCCACCCGGGTGGGCATCGAATGTTCGGTAGCAAA
CGT

>201_SRO7_1632_fwd (20-mer)
AACGTTGAACAAGGCCAAAG

>200_SRO7_1756_rev (20-mer)
CCGCTTTCTGGTCTGTTCTC

>199_T7_PYC2_fwd (63-mer)
CATATGGCCATGGAGGCCAGTGAATTCCACCCGGGTGGGCATCGAATGAGCAGTAGCAAG
AAA

>198_PYC2_1530_fwd (20-mer)
AAATCCAAGTGTCCCCCATT

>197_PYC2_1728_rev (20-mer)
CCAAATCGTGGGTTCTGACT

>196_pMEC_MX4_fwd (19-mer) primers 195 and 196 sit on each side of the integration point of the MX4 cassette in the
acttttcggggaaatgtgc

>195_pMEC_MX4_rev (20-mer)
ataataccgcgccacatagc

>194_PROMrecLAT1fwd3 (60-mer) forward primer for cloning of AmLAT1, incorporates a HindIII site and AAA in front of the start codon
TTCTAGAACTAGTGGATCCCCCGGGCTGCAGCATGGGTCGGTTACCTAAAGTTTACAACC

>193_PROMrecLAT2fwd3 (57-mer) forward primer for cloning of AmLAT2, incorporates a HindIII site and AAA in front of the start codon
TTCTAGAACTAGTGGATCCCCCGGGCTGCAGCATGGGTCAGTTTATTGAAAAATTCA

>192_CYC1promfwd (22-mer)
CAAACACAAATACACACACTAA

>191_ADH1promfwd (24-mer)
TCAAGCTATACCAAGCATACAATC

>190_MumMX4_MunI_fw (65-mer) Primers 189 and 190 are for the integration of a MX4 marker into the mumberg vectors
aatgtgcgcggaacccctatttgtttatttttctaaatacaCaattGCGTACGCTGCAGG
TCGAC

>189_MumMX4rv (64-mer)
atttatcagggttattgtctcatgagcggatacatatttgaaGCATAGGCCACTAGTGGA
TCTG

>188_I-SceIfwd_Acc65I (39-mer)
GATCGGTACCCATATGAAAAACATCAAAAAAAACCAGGT

>187_PROMrecLAT1fwd2 (60-mer)
TTCTAGAACTAGTGGATCCCCCGGGCTGCAGCATGGGTCGGTTACCTAAAGTTTACAACC

>186_CYC1t_recLAT1re (56-mer) reverse primer for cloning of AmLAT1, incorporates a XhoI site after the stop codon
TATCGATAAGCTTGATATCGAATTCCTGCAGTCAAGCAAAATTATGATTCTCATCA

>185_PROMrecLAT2fwd2 (57-mer)
TTCTAGAACTAGTGGATCCCCCGGGCTGCAGCATGGGTCAGTTTATTGAAAAATTCA

>184_CYC1t_recLAT2re (55-mer) reverse primer for cloning of AmLAT2, incorporates a XhoI site after the stop codon
TATCGATAAGCTTGATATCGAATTCCTGCAGTCAAACACTACTTACAGAGTCTTT

>183_I-SceIfwd_Bsp140 (39-mer)
GATCTGTACACATATGAAAAACATCAAAAAAAACCAGGT

>182_I-SceIrev_KpnI (33-mer)
GATCGGTACCTTTCAGGAAAGTTTCGGAGGAGA
GAACTTTTCACT

>181_LAT1fwd (38-mer)
GATCAAGCTTAAAATGCGGTTACCTAAAGTTTACAACC

>180_LAT1rev (34-mer)
GATCTCGAGTCAAGCAAAATTATGATTCTCATCA

>179_TEF1p_rec_LAT1fw (86-mer)
TTTCTAGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGATATCAAGCTTAAAATGGGTC
GGTTACCTAAAGTTTACAACCCATAC

>178_CYC1t_rec_LAT1re (81-mer)
GGATGTGGGGGGAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTCGAGTCAA
GCAAAATTATGATTCTCATCA

>177_TEF1p_rec_LAT2fw (75-mer)
TTTCTAGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGATATCAAGCTTATGGGTCAGT
TTATTGAAAAATTCA

>176_CYC1t_rec_LAT2r (84-mer)
GGATGTGGGGGGAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTCGAGTCAA
ACACTACTTACAGAGTCTTTGAGC

>175_CaART1GFP_fw (72-mer)
GAAAAACCATCTGCTGATCATGTTGAATCCGTTCGGTCTTTAGA TAGTAGTAAAGGAGAA
GAACTTTTCACT

>174_CaART1optGFP_fw (72-mer)
GAAAAACCATCTGCCGACCATGTGGAAAGTGTCAGATCACTGGATTCTAGTAAAGGAGAA
GAACTTTTCACT

>173_Pg3012ART1GFP_fw (72-mer)
GAGGGCATGCTTCAATCGCAAGCCTCATCGGAGGAAAAGCCAACTGTCAGTAAAGGAGAA
GAACTTTTCACT

>172_AmLAT1GFP_fw (72-mer) This primer (72bp) anneals (24bp) to the 5' part of the GFP gene in the pFA6a-GFPS65T-kanMX6 vector. The first 48bp are homologous to the last part of the AmLAT1 gene with the stop codon removed. This primer can be used to create GFP fusions with AmLAT1.
TGTGAAGCTGAACATGTGGAGTCAATTGATGAGAATCATAATTTTGCTAGTAAAGGAGAA
GAACTTTTCACT

>171_AmLAT2GFP_fw (72-mer) This primer (72bp) anneals (24bp) to the 5' part of the GFP gene in the pFA6a-GFPS65T-kanMX6 vector. The first 48bp are homologous to the last part of the AmLAT2 gene with the stop codon removed. This primer can be used to create GFP fusions with AmLAT2.
AATGAAGAAAAGCCTACTGATGAGCTCAAAGACTCTGTAAGTAGTGTTAGTAAAGGAGAA
GAACTTTTCACT

>170_GFPrev2 (78-mer)This primer (72bp) anneals (24bp) to the 5' part of the GFP gene in the pFA6a-GFPS65T-kanMX6 vector. The first 48bp are homologous to the last part of the AmLAT2 gene with the stop codon removed. This primer can be used to create GFP fusions with AmLAT2.
GATGTGGGAGGAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTCGAGCTATT
TGTATAGTTCATCCATGC

>169_pGAD-C3-T7 (78-mer) This primer anneals (21bp) on pGAD-Cx vectors, just before the ClaI site. The first part is homologous to the pGADT7 vector. The purpose is gap repair to transfer inserts from pGAD-Cx vectors to pGADT7 by gap repair.
GACGTACCAGATTACGCTCATATGGCCATGGAGGCCAGTGAATTCCACCCGGGTGGGCCA
AAAAAAGAGATCGAATTC

>168_ADH1termrev (20-mer) This primer anneals in the ADH1 terminator of pGADT7 and pGAD-Cx vectors. This gives a large region of homology for transfer of inserts from pGAD-Cx to pGADT7.
TAGAGGTGTGGTCAATAAGA

>167_pCAPSfw (24-mer)
TCCTGACGGGTAATTTTGATTTGC

>166_pCAPSrv (24-mer) EMPTY
CTGTGAAGTGGCTGAAATTTCGTA

>165_AmLAT1_216_F (25-mer)
TTGGAAGTTTAGTTTCAGGTCTGTT

>164_AmLAT1_724_F (20-mer)
TCGTCGAAATGGAAAAATCC

>163_AmLAT1_1234_F (20-mer)
GATCCCGTGGTACCTCCAGT

>162_AmLAT1_122_R (20-mer)
AATGATGGCGGAAATTGAAC

>161_AmLAT1_562_R (20-mer)
GCAGGGATCATTTGAAGACC

>160_AmLAT1_1078_R (20-mer)
ACGGATTGAGCGTATTCACC

>159_AmLAT2_293_F (20-mer)
CGGTACTCTTTGCGGTTGTT

>158_AmLAT2_767_F (20-mer)
CGAGGAGAGAGGTGCTGAAG

>157_AmLAT2_1288_F (21-mer)
CCATGGATTATCCCATCTGAG

>156_AmLAT2_130_R (20-mer)
GCTGAAAGTGCAACGACAGA

>155_AmLAT2_638_R (20-mer)
TGGAAATCTCCAACGGAAAG

>154_AmLAT2_1149_R (20-mer)
TACCGGTACAACCCCAGAAT

>153_TEF1promfwd (29-mer)
TCTTCAATTTCTCAAGTTTCAGTTTCATT

>152_pHXT7_fwd (20-mer) This primer sits 236 bp from the start codon in the HXT7p in fwd direction
CATCTTTCGAGATCCCCTGT

>151_pTAC_fwd (24-mer) (EMPTY) Forward primer in the pTAC promoter. This primer has been refered to as M13/pUCrev http://www.jbc.org/cgi/content/full/278/1/18 and M13 sequence tag. It also anneals to the pUC19.
GAGCGGATAACAATTTCACACAGG

>150_MX4fwd (25-mer) Primer in the Ashbya gossypi TEF promoter in forward direction. contaminated?
AAAATCTTGCTAGGATACAGTTCTC

>149_MX4rev (25-mer) Primer in the Ashbya gossypi TEF terminator in the reverse direction.
ACAAATGACAAGTTCTTGAAAACAA

>148_LAT1fwd (38-mer)
GATCAAGCTTAAAATGCGGTTACCTAAAGTTTACAACC

>147_LAT1rev (34-mer)
GATCTCGAGTCAAGCAAAATTATGATTCTCATCA

>146_LAT2fwd (38-mer)
GATCAAGCTTAAAATGGGTCAGTTTATTGAAAAATTCA

>145_LAT2rev (37-mer)
GATCTCGAGTCAAACACTACTTACAGAGTCTTTGAGC

>144_ART1fwd (33-mer) forward primer for cloning of CaART1, incorporates a HindIII site and AAA in front of the start codon
GATCAAGCTTAAAATGGTTTTCGGTAACAGGCA

>143_ART2rev (33-mer) reverse primer for cloning of CaART1, incorporates a XhoI site after the stop codon
GATCTCGAGTTAACTATCTAAAGACCGAACGGA

>142_GFP_rv_seq (20-mer) this primer sits 118 bp from the 5' of the mycGFP gene, reverse with respect of the ORF. Useful for sequencing GFP fusions.
GAACTTCAGGGTCAGCTTGC

>141_GFP_fw_seq (20-mer) this primer sits 48 bp from the 3' end of the mycGFP gene, forward with respect of the ORF.
ACATGGTCCTGCTGGAGTTC

>140_HXT7p_fwd (20-mer) despite the name, this primer sits inside the E. coli AraB coding sequence fwd with respect of the ORF.
GAACGCGATGTTCGTATTGT

>139_araB_rev (22-mer) This primer sits inside the E. coli AraB ORF 377 bp from the start in a reverse direction. Useful for detecting the ara B gene
ACGGGTAATCTCTTCCGCTTCT

>138_bstSEINrv_HA_Eco (new)
GATCGAATTCTTAAGCGTAATCCGGAACATCATACGGGTAAAACCTTACCTCCTTGTCAACAAC

>137_bstSEINrv_HA_Eco
GATCGAATTCTTAAGCGTAATCCGGAACATCATACGGGTAAAACCTTACCTCCTTGTCAACAAC

>136_bstSEINfw_Hind
GATCAAGCTTAAAATGGCTAAAAAAGTTAATTGGTATGTTTCT

>135_CCP1_chk
CGCCCAGTCCATTTATTTTC

>134_SSY5_chk
TCCTGGAGAGACTACATGACTGT

>133_PTR3_chk
TCGCGAAATCACCAAATGTA

>132_CYC1-tag-rev(2)
TAGAGCGGATGTGGGAGGAGGGCGTGAATGTACTATAGGGAGACCGGCAG

>131_116CREseq_rev
nnn

>130_A-ZWF1
ATTATTAATGTGGGATTTTTGGCTC

>129_D-ZWF1
TCAATGATAAGTACAAGTCCAATCG

>128_Alpha-ZWF1
nnn

>127_Beta-ZWF1
nnn

>126_AFT1fwd
TTCTAGAACTAGTGGATCCCCCGGGCTGCAG ATGGAAGGCTTCAATCCGG

>125_AFT1rev
TATCGATAAGCTTGATATCGAATTCCTGCAG CTAATCTTCTGGCTTCACA

>124_FRE1fwd
TTCTAGAACTAGTGGATCCCCCGGGCTGCAGATGGTTAGAACCCGTGTAT

>123_FRE1rev
TATCGATAAGCTTGATATCGAATTCCTGCAG TTACCATGTAAAACTTTCT

>122_UTR1fwd
TTCTAGAACTAGTGGATCCCCCGGGCTGCAGATGAAGGAGAATGACATGA

>121_UTR1rev
TATCGATAAGCTTGATATCGAATTCCTGCAGTTATACTGAAAACCTTGCT

>120_ZWF1fwd
TTCTAGAACTAGTGGATCCCCCGGGCTGCAGATGAGTGAAGGCCCCGTCA

>119_ZWF1rev
TATCGATAAGCTTGATATCGAATTCCTGCAGCTAATTATCCTTCGTATCT

>118_TEL2
TGGTGTGTGGGT

>117_TEL1
TGTGGGTGTGGTG

>116_CREseq_rev
ACGGCGTTTCACTTCTGAGT

>115_CREseq_fwd
ATGGTCCTGCTGGAGTTCGT

>114_CRE_fwd_Bsp1407I
GTACTGTACAAGGGTGGAGGTGGAGGATCTATGTCCAATTTACTGACC

>113_Delta-HOG1
TCTGGGTGAGACAGCTATTTAG

>112_Alfa-HOG1
AAGACTTTGCCCTGTATATAATTAAAC

>111_RPS10_5ORFfwd
ACTTTGATCAACAGATCTAC

>110_MM3'AD_LD-ISA
GTGAACTTGCGGGGTTTTTCAGTATCTACGATT

>109_MM5'AD_LD-ISA
CTATTCGATGATGAAGATACCCCACCAAACCC

>108_pUG6seq
CATACGATTTAGGTGACAC

>107_D-HOG1
CTCTTCGGTAAAAGCACAGAATTAG

>106_A-HOG1
GAGATTATTCGGCATTTTGACATAC

>105_Jen1dom3fwd
nnn

>104_Jen1dom2fwd
nnn

>103_Jen1dom1fwd
nnn

>102_Jen1dom3rev
AGTTATGCGGCCGCTGCAGGTCGACGGATCCTTAAACGGTCTCAATATGC

>101_Jen1dom2rev
AGTTATGCGGCCGCTGCAGGTCGACGGATCCTTTTGAACTGTTCTCTTCA

>100_Jen1dom1rev
AGTTATGCGGCCGCTGCAGGTCGACGGATCCTAGTTCCAATTCTGCCAT

>99_CRE_cds_r_BsiWI
GTCAAGCTTCGTACGATCGCCATCTTCCAG

>98_CRE_cds_f_Acc65I
GATCGGTACCATGTCCAATTTACTG

>97_SSY5-tag-reverse
GGCAAATCATCCATCTAGTTGTGGATCAATGTCCATAGGGAGACCGGCAG

>96_SSY5-DHFRtag-fwd
AGGTGATATCCTGGAGAGACTACATGACTGTAACGGTGGAGGTGGAGGAT

>95_PTR3-tag-reverse
AATACCTGTATACCAGAACCTTAAACATACGTATATAGGGAGACCGGCAG

>94_PTR3-DHFRtag-fwd
CGAAATCACCAAATGTAAGCAAATATTCCACAAAGGTGGAGGTGGAGGAT

>93_CCP1-tag-reverse
ACGAAATATAACCAATAAATAATATCTTTCCTCAATAGGGAGACCGGCAG

>92_CCP1-DHFRtag-fwd
ATTTATTTTCAAGACTTTAGAGGAACAAGGTTTAGGTGGAGGTGGAGGAT

>91_Ŗ1-DHFRtag-fwd
CGACTTAATTACCTACTTGAAAAAAGCCTGTGAGGGTGGAGGTGGAGGAT

>90_F3_rev_Pf23II
GATCGGTACCTTAGTCTTTCTTCTCGTAG

>89_F12_rev_Pf23II
GATCGGTACCTTACAATTCCGGTTGTTC

>88_LINK_fwd_Pf23II
GATCCGTACGGGTGGAGGTGGAGGAT

>87_MM3'AD_LD-ISA
GTGAACTTGCGGGGTTTTTCAGTATCTACGATT

>86_MM5'AD_LD-ISA this primer is missing from box
CTATTCGATGATGAAGATACCCCACCAAACCC

>85_3'DNA-BD_Seq_Primer
TAAGAGTCACTTTAAAATTTGTAT

>84_T7fwd
GTAATACGACTCACTATAG

>83_MSW_rev this primer sits inside the loxP of pUG6
GTATAGCGACCAGCATTC

>82_MSW_fwd this primer sits inside the loxP of pUG6
TCCTTGACAGTCTTGACG

>81_fwd_GFP this primer is missing from box
ACTTAGTTTCGAATAAACACACATAAACAAACAAATAGAACTAGTGGATC

>80_rev_GFP
ACATGATATCGACAAAGGAAAAGGGGCCTGTTTATTTGTACAATTCATCC

>79_F[3]rev_NoTag
ATGATATCGACAAAGGAAAAGGGGCCTGTTTTTAGTCTTTCTTCTCGTAG

>78_MCSrev(NEW)
ACCGTCGACATCGATAAGCTTCTG

>77_3'AD-LDamplimer
nnn

>76_5'AD-LDamplimer
nnn

>75_3FLG_rev
TACGTCGACTTAGATCTTATCGTCGTCATC

>74_5YCAclon
GTAGGATCCAAATGAAGATGAGCCTCGAA

>73_5AIFclon
GTAGGATCCAAATGACAATTAACACAAAG

>72_3CYC7flag
TACGTCGACTTACTTATCGTCGTCATCCTTGTAATCCATTTGGCAGCCTTTGTCATATA

>71_3CYC1flag
TACGTCGACTTACTTATCGTCGTCATCCTTGTAATCCATCTCACAGGCTTTTTTCAAGT

>70_GFP_rev
AACATCACCATCTAATTCAAC

>69_Strep_r_re
AGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTCGAGTTACAA

>68_Strep_r_fw
TGGATGATGCCTTAAGACTTATTGAACAACCGGAATTGTAACTCGAGTCA

>67_F31S_test
GGTCATTCTTTGGAAGTACTTGCT

>66_S31_test
GGTCATTCTTTGGAAGTACTTGAA

>65_3-70-mer_DFR1
GGCTGTAAACATGCCACAATTCCTACAATAGGAATCTTTCCTCCAGCCATGGCACCCGCTCCAGCGCCTG

>64_5-70-mer_DFR1
GAGATGGCATCGCTGGCCAAAGGGAAACGTTTCATTATTTTCTGTAGTTAATTAAGGCGCGCCAGATCTG

>63_Degron_C
CTGGTGCAGGCGCTGGAGCG

>62_3CYC7clon
GCATATCGATCTAGTCGACTTTGGCAGCCTTT

>61_5CYC7clon
GATCGAATTCCAAATGGCTAAAGAAAGTACGG

>60_mDHFR_F31S_rev
GTCATTCTTTGGAAGTACTTGCTCTCGTTCCTGAGCGGA

>59_mDHFR_F31S_fwd
TCCGCTCAGGAACGAGAGCAAGTACTTCCAAAGAATGAC

>58_Lumio_Rev_77
GCGTGACATAACTAATTACATGATATCGACAAAGGAAAAGGGGCCTGTTTAGCCACCTCCACAGCAACCTGGGCAAC

>57_Lumio_Fwd_73
AGACAGAAACGACTTAATTACCTACTTGAAAAAAGCCTGTGAGGCTGGTGGATGTTGCCCAGGTTGCTGTGGA

>56_CYC1termrev
GAATGTAAGCGTGACATAACTAATTACATG

>55_TEF2promfwd
TGGTGTCGCTCCTTCTAGTGCTA

>54_TDHpromFWD2
ACAAGGCAATTGACCCACGCATGTATCTA

>53_Syclrev
ATCGGATCCCTCACAGGCTTTTTTCAA

>52_pU-R2
GGGATGTATGGGCTAAATG

>51_TefTermFwd(A.gos (almost the same as kanC3, partly overlaps but ends 15 bp further downstream)
CAGATGCGAAGTTAAGTGCG

>50_TefPromRev(A.gos = kanB)
CTGCAGCGAGGAGCCGTAAT

>49_JEN1HAr
CTTAGACACAGTAATCTACCATCTTGCTAGTGTTAACGGCTGTTATGTTTCTTGCGGC ACTATAGGGAGACCGGCAGATC

>48_JEN1Haf
CAGTGAAGATGATTGATTCGAACGTCTCAAAGACATATGAGGAGCATATTGAGACCGTTTCCCACCACCATCATCATCAC

>47_CYC1-tag-reverse
AGGTTGTCTAACTCCTTCCTTTTCGGTTAGAGCGGATGTGGGAGGAGGGCGTGAATGTACTATAGGGAGACCGGCAGATC

>46_CYC1-tag-forward
GGTTGAAGAAGGAAAAAGACAGAAACGACTTAATTACCTACTTGAAAAAAGCCTGTGAGTCCCACCACCATCATCATCAC

>45_F123rev
GATCCTCGAGTTAGTAATCCGGAACATCATAC

>44_F3:i114a]rev
CTGGTAAACAGAACTGCCTCCGACTGCCCAAACCA

>43_F3:i114a]fwd
GCAAGTAAAGTAGACATGGTTTGGGCAGTCGGAGG

>42_F[3]rev(Cys4)
GATCCTCGAGTTAAGCTCTCGCACAGCATTCTCTGCAACACGCTTCTCTTGCGGCCGCTTCGTCTTTCTTCTCGTAGACTTCA

>41_f[3]rev
GATCCTCGAGTTAGTAATCCGGAACATCATACGGGTATCCGTGATGATGATGGTGGTGGTCTTTCTTCTCGTAGACTTCA

>40_F[3]fwd(SalI)
CGATGTCGACGGTGGAGGTGGAGGATCTGGTGGTGGAGGAGGATCAGCAAGTAAAGTAGACATG

>39_F[3]fwd
GTGGTGGAGGAGGATCAGCAAGTAAAGTAGACATG

>38_F[1,2:p31s]rev
TGTGGTCATTCTTTGGAAGTACTTAGACTCGTTCC

>37_F[1,2:p31s]fwd
CCCTGGCCTCCGCTCAGGAACGAGTCTAAGTACTT

>36_F[1,2]rev(strtg)
GATCCTCGAGTTATTTTTCAAATTGTGGATGAGACCACAATTCCGGTTGTTCAATAAGTCTT

>35_F[1,2]rev
GATCCTCGAGCAATTCCGGTTGTTCAATAAGTCTT

>34_F[1,2]fwd
GTGGTGGAGGAGGATCAGTTCGACCATTGAACTGC

>33_F2fwdN
CGATGTCGACGGTGGAGGTGGAGGATCTGGTGGTGGAGGAGGATCAGCAAGTAAAGTAGACATG

>32_F[1,2]fwd(SalI)
CGATGTCGACGGTGGAGGTGGAGGATCTGGTGGTGGAGGAGGATCAGTTCGACCATTGAACTGCATCGTCG

>31_MCSrev
TGATCCTCCTCCACCACCAGATCCTCCACCTCCACCGTCGACATCGATAAGC

>30_MCSfwd
ACTAGTAGGATCCCGGGATATCGAATTCTGCAGAAGCTTATCGATGTCGACGGTG

>29_PreRev
CCGGGATCCTACTAGTCATAGCTTCGGCAGTTAAG

>28_PREfwd(new)
GATCTCTAGAGCGGCCGCAGAATGTTTTCAAATCTATCTA

>27_NUC1SPREV
TCAAATAAATGTATTATTTTATGTTATATGCGTTAGACTTGTTATATTCTTTCATGCTATTATCGATGAATTCGAGCTCG

>26_NUC1SPFWD
CTAACGCGGCGATCAAACAATCGAAAGATGTGAAATTGTTACCTCCTCCAAAAAAAAGGAATGGTCGACGGATCCCCGGG

>25_Amrev
TGAGGATCCTCATTTACGATTGTATAGAGTGAATT

>24_Amf
TGAGGATCCAAATGGCTGGAGGAAAGATTCCTATT

>23_D-NUC1
AATTCAGAGTTCAAAACAAACAAGG

>22_A-NUC1
GACAAAAATTCATTTAAAGAAGCCA

>21_Delta-DFR1
TAGCAGGATACACTTAGATGACTTAGATAT

>20_Alpha-DFR1
TCTATGGGTGGGTACAAATGGCAGTCTG

>19_D-DFR1
GACTCAGACAGGTTGAAAAGAAGAC

>18_A-DFR1
CAAAGGTTTGGTTTTCAGTTAAGAA

>17_D-CYC7
TTCCTCTACTGCTACTAAGAACGGA

>16_A-CYC7
GTTATAGCGCCCCTTATTGAATTAT

>15_D-CYC1
GATAAATACTCTCTGGAAGCAGACG

>14_A-CYC1
ATCAAGATGTTTTCACCGATCTTT

>13_3ZIPclon
GATCGGATCCTGCGTTCGCCAACTAATTTCTT

>12_5ZIPNw
GATGGATCCGCGGCCGCAAAATGAACACTGAAGCCGCCAGGCGTTCTCGT

>11_5ZIPclon
GATCGCGGCCGCAAATGTGGATCCAAAACACTGAAGCCGCCAGGC

>10_3SNF1clon
ATCGGATCCTCAATTGCTTTGACTGTTAACGGCTAATTCCA

>9_5SNF1clon
GATGGATCCGAATTCAAAATGACGAGTAACAACAACACAAACACA

>8_3NUC1clon
GTAGGATCCTTAGAATTCATTCCTTTTTTTTGGAGGAGGTAACAATTTCACATC

>7_5NUC1clon
GATGGATCCCAAATGTGCAGTAGGATACTCTTG

>6_3NUC1clon(N)
CTAGAGATCTCGAGTTAGAATTCATTCCTTTTTTTTGGAGGAGGTAACAATTTCACATC

>5_5NUC1clon(N)
GATAGATCTAGACTAGTCAAATGTGCAGTAGGATACTCTTG

>4_3CCP1clon
CGATGTCGACTTAGATCTCTAAACCTTGTTCCTCT

>3_5CCP1clon
GATCGGCCGGATCCAAATGACTACTGCTGTTAGGC

>2_3CYC1clon
CGATGTCGACTTAGATCTCACAGGCTTTTTTCAAG

>1_5CYC1clone
GATCGGCCGGATCCAAATGACTGAATTCAAGGCCG

>0_S1 67bp primer for amplification of GFP from pFA6a-GFPS65T-kanMX6. The last 21 bp are specific for the GFP gene in pFA6a-GFPS65T-kanMX6. The rest is homology with the 3'part of ScJEN1. This primer was used for tagging the JEN1 with GFP on the chromosome.
GATTCGAACGTCTCAAAGACATATGAGGAGCATATTGAGACCGTTAGTAAAGGAGAAGAACTTTTC

>GFPrev This primer is a reverse primer specific (22 bp) for the GFP in pFA6a-GFPS65T-kanMX6 with a part homologous to the CYC1 terminator in the MUMBERG vectors. This primer can be used for creating GFP fusions in these vectors by gap repair. This primer was designed using the Ŗ terminator sequence in the SGD database. For this reason, there is only about 36 bp of homology when comparing with the actual sequence.
GGCGTGAATGTAAGCGTGACATAACTAATTACATGATATCGACAAAGGAAAAGGGGCCTGTTAAACAGATCTATATTACCCTG

>HAfwd
GATTCGAACGTCTCAAAGACATATGAGGAGCATATTGAGACCGTTTCCCACCACCATCATCATCAC

>HArev
GGCGTGAATGTAAGCGTGACATAACTAATTACATGATATCGACAAAGGAAAAGGGGCCTGTTCATTAAGCGTAGTCTGGGA

>Haubiqrev
AGCGTAATCCGGAACATCATAC

>GFP_rev
AACATCACCATCTAATTCAAC

>GALprom
GTTAAAGTATAGAGGTATATTAACAAT

>A-YCR010c
AGACTGCATTTTCTTACAGCTTTTT

>D-YCR010c
AGACAAGTAAAGGAGTCAGCAAAAA

>A-JEN1
TATTCTCCACCAGGAAGTTAGTTTG

>D-JEN1
ATTGTCTTATAGTGGCAAAAGCAAG

>A-YGR067c
TATGCTTACAACGCAAAATAATTGA

>D-YGR067c
TGTTATCGAATGTGAACAGAACACT

>GALprom_c
ATTGTTAATATACCTCTATACTTTAAC

>CIp-20_fw_lo Primers for the fusion PCR assembly of CIp20-CaJEN2
AGCCACTGGCACAATCGGTTAGAAATATGAGACGGGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTA

>CaJEN2_rv_lo
AAAGGGAACAAAAGCTGGGTACCGGGCCCCCCCTCGAGGTCGACCCGTCTCATATTTCTAACCGATTGTGCCAGTGGCTC

>CaJEN2_fw_sh
CTTGTTTTTACCGACAGCCATGTTGTACTTGAGTTGGATCTACGCGTGAGCACTAACAATTAGTTGTACAGTTCAAAACT

>CIp-20_rv_sh
GAGTTTTGAACTGTACAACTAATTGTTAGTGCTCACGCGTAGATCCAACTCAAGTACAACATGGC

>CIp-20_rv_lo_n
CCTCATGGTTTGGATTTCACTTCTGACAAATTAAGATCATTGGTCAGAAA

>CIp-20_rv_lo
GATGAAGTCCAAGGTAAAAACTTGTTGACCAACTTCCATG

>CIp-20_fw_sh_n
CCTCATGGAAGTTGGTCAACAAGTTTTTACCTTGGACTTCATCAAC

>CIp-20_fw_sh
TGTCAGAAGTGAAATCCAAACCATG

>jen2_416Rev_REQ primers for the p416GPD-CaJEN2
CATGACTCGAGGTCGACGGTATCGATAAGCTTTTAGTGATGGTGATGGTGATGCTCTTTATGTTCAACTTCTGGTTTTTC

>jen2_416For_REQ
AAAACACCAAGAACTTAGTTTCGACGGATTCTAGAACTAGTGGATCCAATATTATGACTGCTGCTGATACTCATTCTATC

>NewCaJEN2-DB4
TTCAAGATCAGAATCACCTCTGTCTTCCCTATCACTATCGTACGCACTGTATTCGTCATCTGTGGAATTGTGAGCGGATA

>NewCaJEN2-DB3
CATAATAGACACATTATTCGTCCACCAAAATTCACTTGGCCCGCTATTCGAAAATATGCCTTTTCCCAGTCACGACGTT

>URA3-dpl200rev KlURA3?
ACCATGATTACGCCAAGCTC

>URA3-dpl200fwd KlURA3?
TAAAACGACGGCCAGTGAAT

>120_Ca5
TTCAATATTTGAATAGGGTAAAAGC

>119_Ca4
AACTTAAAATGCTATTATTGGAAAAGG

>2u-CENARS_AarI_f (34-mer)
cctttGCAGGTGgtatttcacaccgcatatatcg

>2u-CENARS_AarI_r (37-mer)
cctttGCAGGTGttttgaaaagcaagcataaaagatc

>CORE_JEN1_I-SceI_fwd Primers to amplify the pCORE casette
ACAGTTTCAAAAGTTTTTCCTCAAAGAGATTAAATACTGCTACTGAAAATTAGGGATAACAGGGTAATTTGGATGGACGCAAAGAAGT

>CORE_JEN1_rev
CAACCACGCAAAATAACCCATAAAAAAATAGTTCCAATTCTGCCATGTCATTCGTACGCTGCAGGTCGAC

>Syntforw Primers to amplify the synthetic 399 bp gene. Desenhei o primer forw 18pb JEN1+ 50pb upstream JEN1 Desenhei primer forw 50 pb depois da lisina posição (399aa) +50pb depois
ACAGTTTCAAAAGTTTTTCCTCAAAGAGATTAAATACTGCTACTGAAAATATGTCGTCGTCAATTACAG

>Synthrev_
CAACCACGCAAAATAACCCATAAAAAAATAGTTCCAATTCTGCCATGTCATTCTGCGCAGTTCGGGTATG

>242b_CaART1opt_1246_F (20-mer)
TTAGGTCAAAGGGCATGAGC

>241b_CaART1opt_143_R (20-mer)
TGTTCCCAAGATGGCACTTA




