LOCUS       pUC_LAC4__copy_         6389 bp ds-DNA     circular     28-JAN-2014
DEFINITION  Cloning vector pUC19c, complete sequence.
ACCESSION   L09137 X02514
VERSION     L09137.2  GI:20141090
KEYWORDS    .
SOURCE      Cloning vector pUC19c
  ORGANISM  Cloning vector pUC19c other sequences; _artificial_ sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Yanisch-Perron,C., Vieira,J. and Messing,J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
   PUBMED   2985470
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
  TITLE     The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
            to facilitate the use of sonicated DNA for nucleotide sequencing
  JOURNAL   Gene 68 (1), 139-149 (1988)
   PUBMED   2851488
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Department of Biochemistry, University of
            Minnesota, St. Paul, MN 55108, USA
REFERENCE   5  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2002) Rutgers, The State University of New
            Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
            Piscataway, NJ 08854-8020, USA
  REMARK    Sequence update by submitter
COMMENT     On Apr 11, 2002 this sequence version replaced gi:209213. These
            data and their annotation were supplied to GenBank by Will Gilbert
            under the auspices of the GenBank Currator Program. pUC19c -
            Cloning vector (beta-galactosidase mRNA on complementary strand)
            ENTRY PUC19C                    #TYPE DNA CIRCULAR TITLE pUC19c -
            Cloning vector               (beta-galactosidase mRNA on
            complementary strand) DATE      03-FEB-1986    #sequence
            16-DEC-1986 ACCESSION VB0033 SOURCE    artificial COLLECTION  ATCC
            37254 REFERENCE    #number    #authors  Norrander J., Kempe T.,
            Messing J.    #journal Gene (1983) 26: 101-106 REFERENCE    #number
            1    #authors  Yanisch-Perron C., Vieira J., Messing J.    #journal
            Gene (1985) 33: 103-119    #comment shows the complete compiled
            sequence REFERENCE    #number  2    #authors  Chambers,S.P., et al.
            #journal Gene (1988) 68: 139-149    #describes mutation at nt1308
            and its effect on copy number REFERENCE    #number    #authors
            Pouwels P.H., Enger-Valk B.E., Brammar W.J.    #book    Cloning
            Vectors, Elsvier 1985 and supplements    #comment  vector I-A-iv-20
            COMMENT    This Sequence was obtained 3-MAR-1986 from J. Messing,
            Waksman    Institute, NJ on floppy disk.    Revised 16-DEC-1986 by
            F. Pfeiffer:    1062/3 'AT' to 'TA' to match revised sequence of
            PBR322    The beta-galactosidase mRNA sequence including the
            multiple    cloning site of M13mp19 is on the strand complementary
            to that    shown. KEYWORDS CROSSREFERENCE    #complement
            VecBase(3):pUC19    #prerevised      GenBank(50):M11662,
            EMBL(11):ARPuc19    #parent      VecBase(3):pUC13,
            VecBase(3):M13mp19, VecSource(3):bGal19 PARENT    Features of
            pUC19c (2686 bp)      residue     source       1- 137  2074-2210
            pBR322     138- 237  2252-2351 pBR322     238- 395  1461-1304 (c)
            Lac-Operon     396- 452    57-   1 (c) polylinker of M13mp19 455-
            682  1298-1071 (c) Lac-Operon     683-2686  2352-4355 pBR322
            Conflict (cfl) and Mutations (mut):         pUC19c  source    mut
            1308  A  G  2977   pBR322   linked to increased copy number    mut
            1942  A  G  3611   pBR322    mut 2243  T  C  3912   pBR322 FEATURE
            1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER
            HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION
            #resistance  Ap    #indicator beta-galactosidase SUMMARY  pUC19c
            #length 2686   #checksum 4465.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(4118..6389,1..414)
                     /organism="Cloning vector pUC19c"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:174689"
                     /label=source:Cloning vector pUC19c
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          415..4117
                     /organism="Kluyveromyces lactis"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:28985"
                     /tissue_lib="CBS2359"
                     /label=source:Kluyveromyces lactis
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            423..3820
                     /gene="LAC4"
                     /label=LAC4
                     /ApEinfo_fwdcolor=#ffc02c
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     TATA_signal     423..426
                     /gene="LAC4"
                     /note="putative"
                     /label=LAC4(1)
                     /ApEinfo_label=LAC4
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             457..3534
                     /gene="LAC4"
                     /EC_number="3.2.1.23"
                     /codon_start=1
                     /product="beta-D-galactosidase"
                     /protein_id="AAA35265.1"
                     /db_xref="GI:173305"
                     /translation="MSCLIPENLRNPKKVHENRLPTRAYYYDQDIFESLNGPWAFALF
                     DAPLDAPDAKNLDWETAKKWSTISVPSHWELQEDWKYGKPIYTNVQYPIPIDIPNPPT
                     VNPTGVYARTFELDSKSIESFEHRLRFEGVDNCYELYVNGQYVGFNKGSRNGAEFDIQ
                     KYVSEGENLVVVKVFKWSDSTYIEDQDQWWLSGIYRDVSLLKLPKKAHIEDVRVTTTF
                     VDSQYQDAELSVKVDVQGSSYDHINFTLYEPEDGSKVYDASSLLNEENGNTTFSTKEF
                     ISFSTKKNEETAFKINVKAPEHWTAENPTLYKYQLDLIGSDGSVIQSIKHHVGFRQVE
                     LKDGNITVNGKDILFRGVNRHDHHPRFGRAVPLDFVVRDLILMKKFNINAVRNSHYPN
                     HPKVYDLFDKLGFWVIDEADLETHGVQEPFNRHTNLEAEYPDTKNKLYDVNAHYLSDN
                     PEYEVAYLDRASQLVLRDVNHPSIIIWSLGNEACYGRNHKAMYKLIKQLDPTRLVHYE
                     GDLNALSADIFSFMYPTFEIMERWRKNHTDENGKFEKPLILCEYGHAMGNGPGSLKEY
                     QELFYKEKFYQGGFIWEWANHGIEFEDVSTADGKLHKAYAYGGDFKEEVHDGVFIMDG
                     LCNSEHNPTPGLVEYKKVIEPVHIKIAHGSVTITNKHDFITTDHLLFIDKDTGKTIDV
                     PSLKPEESVTIPSDTTYVVAVLKDDAGVLKAGHEIAWGQAELPLKVPDFVTETAEKAA
                     KINDGKRYVSVESSGLHFILDKLLGKIESLKVKGKEISSKFEGSSITFWRPPTNNDEP
                     RDFKNWKKYNIDLMKQNIHGVSVEKGSNGSLAVVTVNSRISPVVFYYGFETVQKYTIF
                     ANKINLNTSMKLTGEYQPPDFPRVGYEFWLGDSYESFEWLGRGPGESYPDKKESQRFG
                     LYDSKDVEEFVYDYPQENGNHTDTHFLNIKFEGAGKLSIFQKEKPFNFKISDEYGVDE
                     AAHACDVKRYGRHYLRLDHAIHGVGSEACGPAVLDQYRLKAQDFNFEFDLAFE"
                     /label=beta-D-galactosidase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    3815..3820
                     /gene="LAC4"
                     /label=LAC4(2)
                     /ApEinfo_label=LAC4
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccAAAAAA
      421 AAAATAAACA CACATACTCA TCGAGAACTG AAAGATATGT CTTGCCTTAT TCCTGAGAAT
      481 TTAAGGAACC CCAAAAAGGT TCACGAAAAT AGATTGCCTA CTAGGGCTTA CTACTATGAT
      541 CAGGATATTT TCGAATCTCT CAATGGGCCT TGGGCTTTTG CGTTGTTTGA TGCACCTCTT
      601 GACGCTCCGG ATGCTAAGAA TTTAGACTGG GAAACGGCAA AGAAATGGAG CACCATTTCT
      661 GTGCCATCCC ATTGGGAACT TCAGGAAGAC TGGAAGTACG GTAAACCAAT TTACACGAAC
      721 GTACAGTACC CTATCCCAAT CGACATCCCA AATCCTCCCA CTGTAAATCC TACTGGTGTT
      781 TATGCTAGAA CTTTTGAATT AGATTCGAAA TCGATTGAGT CGTTCGAGCA CAGATTGAGA
      841 TTTGAGGGTG TGGACAATTG TTACGAGCTT TATGTTAATG GTCAATATGT GGGTTTCAAT
      901 AAGGGGTCCC GTAACGGGGC TGAATTTGAT ATCCAAAAGT ACGTTTCTGA GGGCGAAAAC
      961 TTAGTGGTCG TCAAGGTTTT CAAGTGGTCC GATTCCACTT ATATCGAGGA CCAAGATCAA
     1021 TGGTGGCTCT CTGGTATTTA CAGAGACGTT TCTTTACTAA AATTGCCTAA GAAGGCCCAT
     1081 ATTGAAGACG TTAGGGTCAC TACAACTTTT GTGGACTCTC AGTATCAGGA TGCAGAGCTT
     1141 TCTGTGAAAG TTGATGTCCA GGGTTCTTCT TATGATCACA TCAATTTCAC ACTTTACGAA
     1201 CCTGAAGATG GATCTAAAGT TTACGATGCA AGCTCTTTGT TGAACGAGGA GAATGGGAAC
     1261 ACGACTTTTT CAACTAAAGA ATTTATTTCC TTCTCCACCA AAAAGAACGA AGAAACAGCT
     1321 TTCAAGATCA ACGTCAAGGC CCCAGAACAT TGGACCGCAG AAAATCCTAC TTTGTACAAG
     1381 TACCAGTTGG ATTTAATTGG ATCTGATGGC AGTGTGATTC AATCTATTAA GCACCATGTT
     1441 GGTTTCAGAC AAGTGGAGTT GAAGGACGGT AACATTACTG TTAATGGCAA AGACATTCTC
     1501 TTTAGAGGTG TCAACAGACA TGATCACCAT CCAAGGTTCG GTAGAGCTGT GCCATTAGAT
     1561 TTTGTTGTTA GGGACTTGAT TCTAATGAAG AAGTTTAACA TCAATGCTGT TCGTAACTCG
     1621 CATTATCCAA ACCATCCTAA GGTGTATGAC CTCTTCGATA AGCTGGGCTT CTGGGTCATT
     1681 GACGAGGCAG ATCTTGAAAC TCATGGTGTT CAAGAGCCAT TTAATCGTCA TACGAACTTG
     1741 GAGGCTGAAT ATCCAGATAC TAAAAATAAA CTCTACGATG TTAATGCCCA TTACTTATCA
     1801 GATAATCCAG AGTACGAGGT CGCGTACTTA GACAGAGCTT CCCAACTTGT CCTAAGAGAT
     1861 GTCAATCATC CTTCGATTAT TATCTGGTCC TTGGGTAACG AAGCTTGTTA TGGCAGAAAC
     1921 CACAAAGCCA TGTACAAGTT AATTAAACAA TTGGATCCTA CCAGACTTGT GCATTATGAG
     1981 GGTGACTTGA ACGCTTTGAG TGCAGATATC TTTAGTTTCA TGTACCCAAC ATTTGAAATT
     2041 ATGGAAAGGT GGAGGAAGAA CCACACTGAT GAAAATGGTA AGTTTGAAAA GCCTTTGATC
     2101 TTGTGTGAGT ACGGCCATGC AATGGGTAAC GGTCCTGGCT CTTTGAAAGA ATATCAAGAG
     2161 TTGTTCTACA AGGAGAAGTT TTACCAAGGT GGCTTTATCT GGGAATGGGC AAATCACGGT
     2221 ATTGAATTCG AAGATGTTAG TACTGCAGAT GGTAAGTTGC ATAAAGCTTA TGCTTATGGT
     2281 GGTGACTTTA AGGAAGAGGT TCATGACGGA GTGTTCATCA TGGATGGTTT GTGTAACAGT
     2341 GAGCATAATC CTACTCCGGG CCTTGTAGAG TATAAGAAGG TTATTGAACC CGTTCATATT
     2401 AAAATTGCGC ACGGATCTGT AACAATCACA AATAAGCACG ACTTCATTAC GACAGACCAC
     2461 TTATTGTTTA TCGACAAGGA CACGGGAAAG ACAATCGACG TTCCATCTTT AAAGCCAGAA
     2521 GAATCTGTTA CTATTCCTTC TGATACAACT TATGTTGTTG CCGTGTTGAA AGATGATGCT
     2581 GGTGTTCTAA AGGCAGGTCA TGAAATTGCC TGGGGCCAAG CTGAACTTCC ATTGAAGGTA
     2641 CCCGATTTTG TTACAGAGAC AGCAGAAAAA GCTGCGAAGA TCAACGACGG TAAACGTTAT
     2701 GTCTCAGTTG AATCCAGTGG ATTGCATTTT ATCTTGGACA AATTGTTGGG TAAAATTGAA
     2761 AGCCTAAAGG TCAAGGGTAA GGAAATTTCC AGCAAGTTTG AGGGTTCTTC AATCACTTTC
     2821 TGGAGACCTC CAACGAATAA TGATGAACCT AGGGACTTTA AGAACTGGAA GAAGTACAAT
     2881 ATTGATTTAA TGAAGCAAAA CATCCATGGA GTGAGTGTCG AAAAAGGTTC TAATGGTTCT
     2941 CTAGCTGTAG TCACGGTTAA CTCTCGTATA TCCCCAGTTG TATTTTACTA TGGGTTTGAG
     3001 ACTGTTCAGA AGTACACGAT CTTTGCTAAC AAAATAAACT TGAACACTTC TATGAAGCTT
     3061 ACTGGCGAAT ATCAGCCTCC TGATTTCCCA AGAGTTGGGT ACGAATTCTG GCTAGGAGAT
     3121 AGTTATGAAT CATTTGAATG GTTAGGTCGC GGGCCCGGCG AATCATATCC GGATAAGAAG
     3181 GAATCTCAAA GATTCGGTCT TTACGATTCC AAAGATGTAG AGGAATTCGT ATATGACTAT
     3241 CCTCAAGAAA ATGGAAATCA TACAGATACC CACTTTTTGA ACATCAAATT TGAAGGTGCA
     3301 GGAAAACTAT CGATCTTCCA AAAGGAGAAG CCATTTAACT TCAAGATTTC AGACGAATAC
     3361 GGGGTTGATG AAGCTGCCCA CGCTTGTGAC GTTAAAAGAT ACGGCAGACA CTATCTAAGG
     3421 TTGGACCATG CAATCCATGG TGTTGGTAGC GAAGCATGCG GACCTGCTGT TCTGGACCAG
     3481 TACAGATTGA AAGCTCAAGA TTTCAACTTT GAGTTTGATC TCGCTTTTGA ATAAGAATTT
     3541 TATACTTAGA TAAGTATGTA CTTACAGGTA TATTTCTATG AGATACTGAT GTATACATGC
     3601 ATGATAATAT TTAAACGGTT ATTAGTGCCG ATTGTCTTGT GCGATAATGA CGTTCCTATC
     3661 AAAGCAATAC ACTTACCACC TATTACATGG GCCAAGAAAA TATTTTCGAA CTTGTTTAGA
     3721 ATATTAGCAC AGAGTATATG ATGATATCCG TTAGATTATG CATGATTCAT TCCTACAACT
     3781 TTTTCGTAGC ATAAGGATTA ATTACTTGGA TGCCAATAAA AAAAAAAAAC ATCGAGAAAA
     3841 TTTCAGCATG CTCAGAAACA ATTGCAGTGT ATCAAAGTAA AAAAAAGATT TTCACTACAT
     3901 GTTCCTTTTG AAGAAAGAAA ATCATGGAAC ATTAGATTTA CAAAAATTTA ACCACCGCTG
     3961 ATTAACGATT AGACCGTTAA GCGCACAACA GGTTATTAGT ACAGAGAAAG CATTCTGTGG
     4021 TGTTGCCCCG GACTTTCTTT TGCGACATAG GTAAATCGAA TACCATCATA CTATCTTTTC
     4081 CAATGACTCC CTAAAGAAAG ACTCTTCTTC GATGTTGggg gatcctctag agtcgacctg
     4141 caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4201 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4261 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4321 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4381 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4441 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4501 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4561 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4981 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5101 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5161 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5221 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5281 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5341 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5401 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5461 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5521 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5581 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5641 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5701 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5761 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5821 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5881 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5941 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6001 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6061 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6301 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6361 aaataggcgt atcacgaggc cctttcgtc   
//
