LOCUS       .                       3683 bp    DNA     linear   UNK 10-JAN-2013
DEFINITION  GUP1_locus
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(383..604)
                     /note="ARS716; Autonomously Replicating Sequence"
                     /db_xref="SGD:S000118456"
     gene            1001..2683
                     /locus_tag="YGL084C"
                     /gene="GUP1"
     mRNA            1001..2683
                     /locus_tag="YGL084C"
                     /gene="GUP1"
                     /product="Gup1p"
     CDS             1001..2683
                     /product="Gup1p"
                     /GO_component="GO:0005783 - endoplasmic reticulum [Evidence
                     IDA] [PMID 15813700]"
                     /GO_component="GO:0016021 - integral to membrane [Evidence
                     IEA]"
                     /GO_component="GO:0016021 - integral to membrane [Evidence
                     ISM] [PMID 12192589]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA,IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IDA,ISS]
                     [PMID 10931309]"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IDA]
                     [PMID 15813700]"
                     /GO_function="GO:0008374 - O-acyltransferase activity
                     [Evidence IMP] [PMID 16597698]"
                     /GO_function="GO:0008374 - O-acyltransferase activity
                     [Evidence IMP] [PMID 18036137]"
                     /GO_function="GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start="1"
                     /locus_tag="YGL084C"
                     /note="Plasma membrane protein involved in remodeling GPI
                     anchors; member of the MBOAT family of putative
                     membrane-bound O-acyltransferases; proposed to be involved
                     in glycerol transport"
                     /db_xref="SGD:S000003052"
                     /GO_process="GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IMP] [PMID 16597698]"
                     /GO_process="GO:0019563 - glycerol catabolic process
                     [Evidence IGI,IMP] [PMID 10747858]"
                     /GO_process="GO:0015793 - glycerol transport [Evidence
                     IGI,IMP] [PMID 10931309]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /ApEinfo_revcolor="#daeaab"
                     /translation="MSLISILSPLITSEGLDSRIKPSPKKDASTTTKPSLWKTTEFKFY
                     YIAFLVVVPLMFYAGLQASSPENPNYARYERLLSQGWLFGRKVDNSDSQYRFFRDNFAL
                     LSVLMLVHTSIKRIVLYSTNITKLRFDLIFGLIFLVAAHGVNSIRILAHMLILYAIAHV
                     LKNFRRIATISIWIYGISTLFINDNFRAYPFGNICSFLSPLDHWYRGIIPRWDVFFNFT
                     LLRVLSYNLDFLERWENLQKKKSPSYESKEAKSAILLNERARLTAAHPIQDYSLMNYIA
                     YVTYTPLFIAGPIITFNDYVYQSKHTLPSINFKFIFYYAVRFVIALLSMEFILHFLHVV
                     AISKTKAWENDTPFQISMIGLFNLNIIWLKLLIPWRLFRLWALLDGIDTPENMIRCVDN
                     NYSSLAFWRAWHRSYNKWVVRYIYIPLGGSKNRVLTSLAVFSFVAIWHDIELKLLLWGW
                     LIVLFLLPEIFATQIFSHYTDAVWYRHVCAVGAVFNIWVMMIANLFGFCLGSDGTKKLL
                     SDMFCTVSGFKFVILASVSLFIAVQIMFEIREEEKRHGIYLKC"
                     /gene="GUP1"
                     /protein_id="DAA08021.1"
                     /ApEinfo_fwdcolor="#daeaab"
     gene            complement(2859..3683)
                     /locus_tag="YGL085W"
                     /gene="LCL3"
     mRNA            complement(2859..3683)
                     /locus_tag="YGL085W"
                     /gene="LCL3"
                     /product="Lcl3p"
     CDS             complement(2859..3683)
                     /product="Lcl3p"
                     /GO_component="GO:0016021 - integral to membrane [Evidence
                     IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA,IEA]"
                     /GO_component="GO:0005739 - mitochondrion [Evidence
                     IEA,IEA]"
                     /GO_component="GO:0005739 - mitochondrion [Evidence IDA]
                     [PMID 14562095]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     ND]"
                     /GO_function="GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start="1"
                     /locus_tag="YGL085W"
                     /note="hypothetical protein; mutant has long chronological
                     lifespan; has homology to Staphylococcus aureus nuclease;
                     GFP-fusion protein localizes to mitochondria; is induced in
                     response to the DNA-damaging agent MMS"
                     /db_xref="SGD:S000003053"
                     /GO_process="GO:0008150 - biological_process [Evidence ND]"
                     /translation="MREGDSNSKKSADVAVLSIILTGSTLTLIYTYKRYLTQFKRTNDI
                     PRRIFRKHWLYGKVTSVGDGDNFHFFHMPGGIRGGWGWLRPVPQMIKNDSTAEKLVGDS
                     RNMRFFNFNWITHGRSTKSKIQKAKSQFLKLNVPYKNRKNLPTIPIRLCGIDAPERAHF
                     GNPAQPFGNEALIWLQNRILGKKVWVKPLSIDQYNRCVARVSYWDWFGGWKDLSLEMLK
                     DGLAVVYEGKVNTEFDDREDKYRYYEFLARSRKKGLWIQNKFETPGEYKKRI"
                     /gene="LCL3"
                     /protein_id="DAA08020.1"
ORIGIN
        1 acgcttcttg tattaatact ttatctctgg atccagattt ggatttaata ataccatagt
       61 gtaacaaata gttttcgaac tgcttcttgt caaacatgaa tatcgatact ttggaaggtg
      121 aagatgaaga tgaagatttg ggacgaccgg tgtaaatgct ccatggttca gctgtgaaag
      181 taggtgatga tgaaaatgaa tattttgacg tgatacccgt ctttgaggac caaaacatca
      241 tccctgctca atcaaagtgt gtcctatttt ccttaatgat acgatattcg aagttacact
      301 gttttgaata cttttgtgcg ctttaatatg tgcaagattt atttgttttg atattaactt
      361 ctttttggct tctatttttt caagactgca gtaccctttt tttcacgcaa cttcaggttc
      421 gaaccgaaga atagtttaat tacgtttagt tttcctactg aaactttttt tttaaaagac
      481 cctgagaatt tcatctcgag aataatgaag accaatatat aagtaatgaa tcataaaagt
      541 acaagagcag gatcaaagtt gaatgtctca acaacaggac aagaaaatga gtgaagaagg
      601 agcatatgta cgggtgagca tttttcttgt ataaacataa actagctgaa catcagctca
      661 atcggacata aaaataagtt tccaattaga catgcacttg aacttgtaat tatttatcga
      721 ccaaaaagag taagttaatt ttcacataat cttggaaaag ccagaaacaa gattcgagag
      781 ctggagaatg gatgatgaat caagcaatgt tgaacaatca aaataatata gggtgtatag
      841 gtgttcacgt atttctcttt atgtttagtg ggtgtgccaa aaaggacact gaaagggtaa
      901 gaataataac ttagcatatt gctttcgata tatagatgtt aatcatacaa aggcaaaaac
      961 aaagcgtgaa tcagcatttg ttaacagaca attgcaagaa atgtcgctga tcagcatcct
     1021 gtctccccta attacttccg agggcttaga ttcaagaatc aaaccttcac caaaaaagga
     1081 tgcctctact accactaagc catcactatg gaaaactact gagttcaaat tctactacat
     1141 tgcatttctg gtcgtggttc ccttgatgtt ctatgctggg ttacaagcta gttcgcccga
     1201 aaatccaaac tatgcaagat acgaacgtct cctatctcaa ggttggttat ttggcagaaa
     1261 agtagacaat agtgattctc aatataggtt tttcagggac aattttgcgc tattgtcagt
     1321 tttaatgcta gtccacactt ctataaaacg cattgtactt tattcaacaa atatcactaa
     1381 attgaggttt gatctgatat ttggtttgat ctttttagtg gccgctcatg gtgtcaattc
     1441 gataagaatt ttagcccata tgctaatttt atatgccatc gcccatgtac taaagaactt
     1501 tagaagaata gccaccatca gcatttggat ttatggtatt tctacgcttt ttattaacga
     1561 caacttcaga gcatatccat ttggtaatat ttgctctttt ttaagcccat tggaccattg
     1621 gtatagaggt atcattccaa gatgggatgt ctttttcaat tttactcttt tgagagtctt
     1681 aagttacaac ttggacttct tagagaggtg ggagaattta caaaagaaga aaagtccatc
     1741 ctatgaatca aaagaagcta aatcagccat tttgctcaat gaacgtgcta gattaactgc
     1801 tgcacacccc atacaggact acagcttaat gaattatatt gcatatgtta cttacacgcc
     1861 acttttcatt gccggcccca ttataacatt caatgattat gtttaccaat cgaaacatac
     1921 cttgccatca ataaatttca aattcatttt ttactatgcg gtgagattcg ttattgctct
     1981 cttatctatg gagttcattt tacactttct ccacgttgtg gcaatctcaa aaaccaaagc
     2041 gtgggaaaat gacacacctt tccagatttc catgattggc ttatttaatt tgaatattat
     2101 ttggctaaaa ctactgattc cgtggaggct gtttaggctg tgggctttgc tagacggaat
     2161 cgatacacct gaaaatatga tcaggtgtgt tgataacaat tacagttcac tagcattctg
     2221 gagagcttgg catagaagct acaataagtg ggttgtccgt tacatatata ttcctctagg
     2281 tggttcaaaa aatagagttt tgacatcact agcagtcttt tccttcgtag ctatatggca
     2341 tgacatcgaa ctaaagttat tattatgggg ttggctaata gttttgttcc tcttaccaga
     2401 aatttttgct acccaaattt tctctcatta taccgacgca gtctggtaca gacacgtttg
     2461 cgctgtcggt gctgttttca acatatgggt tatgatgatc gctaatcttt ttggattctg
     2521 cttgggctct gacggtacta aaaaattact aagcgatatg ttctgtaccg tatctggttt
     2581 caaatttgta attttggcaa gcgttagttt attcatcgca gtacaaataa tgtttgaaat
     2641 cagagaagaa gaaaagaggc acggaattta cctaaaatgc tgaatgccaa atttacgaat
     2701 atcaattgta taacactgct atcatgtatg tatttttatc tactagtaca aaaatgaaac
     2761 cgaaaataaa taaaaaaata tcgagaggaa gcgtgaaaag ctaatgaaca tataatatga
     2821 aaatctgcag tatgatggat aaaaatcctc tcgaaaactc aaatacgttt tttatattca
     2881 ccaggcgttt caaatttatt ctggatccac agtccctttt ttctagatct tgccaaaaat
     2941 tcataatatc gatacttatc ttctctatca tcaaattctg tgtttacttt accctcataa
     3001 acaaccgcta atccatcctt gagcatttct aagcttaagt ctttccaccc accaaaccag
     3061 tcccagtatg acactcgagc tacacaacgg ttgtactgat ctatagataa aggcttgacc
     3121 catactttct ttcccaaaat tcggttttgt aaccaaatta aagcttcatt accaaacggt
     3181 tgagctgggt taccgaaatg tgctctttct ggggcatcga ttccacataa tcttatagga
     3241 atagttggca ggtttttcct atttttgtaa ggtacattta gcttcaagaa ctggctttta
     3301 gctttttgta ttttactttt ggtggacctc ccatgcgtaa tccaattgaa attgaaaaac
     3361 ctcatatttc tgctatctcc aacaagcttt tctgccgtag aatcattctt gatcatttgt
     3421 gggacgggtc gtaaccatcc ccatccacct cttataccac ctggcatgtg aaagaaatga
     3481 aagttgtccc cgtcacctac agacgtgact ttaccataaa gccagtgttt acgaaatatt
     3541 cttcgcggta tatcatttgt tcttttgaat tgagttaaat accttttata tgtgtagatc
     3601 aatgttaagg tcgaacccgt aaggatgatg gagaggacag caacatccgc agattttttt
     3661 gaattagaat caccttccct cat
//
