LOCUS       pUC19                   2733 bp ds-DNA     circular     18-OCT-2012
DEFINITION  Cloning vector pUC19c, complete sequence.
ACCESSION   L09137 X02514
VERSION     L09137.2  GI:20141090
KEYWORDS    .
SOURCE      Cloning vector pUC19c
  ORGANISM  Cloning vector pUC19c other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Yanisch-Perron,C., Vieira,J. and Messing,J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
   PUBMED   2985470
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
  TITLE     The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
            to facilitate the use of sonicated DNA for nucleotide sequencing
  JOURNAL   Gene 68 (1), 139-149 (1988)
   PUBMED   2851488
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Department of Biochemistry, University of
            Minnesota, St. Paul, MN 55108, USA
REFERENCE   5  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2002) Rutgers, The State University of New
            Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
            Piscataway, NJ 08854-8020, USA
  REMARK    Sequence update by submitter
COMMENT     On Apr 11, 2002 this sequence version replaced gi:209213. These
            data and their annotation were supplied to GenBank by Will Gilbert
            under the auspices of the GenBank Currator Program. pUC19c -
            Cloning vector (beta-galactosidase mRNA on complementary strand)
            ENTRY PUC19C                    #TYPE DNA CIRCULAR TITLE pUC19c -
            Cloning vector               (beta-galactosidase mRNA on
            complementary strand) DATE      03-FEB-1986    #sequence
            16-DEC-1986 ACCESSION VB0033 SOURCE    artificial COLLECTION  ATCC
            37254 REFERENCE    #number    #authors  Norrander J., Kempe T.,
            Messing J.    #journal Gene (1983) 26: 101-106 REFERENCE    #number
            1    #authors  Yanisch-Perron C., Vieira J., Messing J.    #journal
            Gene (1985) 33: 103-119    #comment shows the complete compiled
            sequence REFERENCE    #number  2    #authors  Chambers,S.P., et al.
            #journal Gene (1988) 68: 139-149    #describes mutation at nt1308
            and its effect on copy number REFERENCE    #number    #authors
            Pouwels P.H., Enger-Valk B.E., Brammar W.J.    #book    Cloning
            Vectors, Elsvier 1985 and supplements    #comment  vector I-A-iv-20
            COMMENT    This Sequence was obtained 3-MAR-1986 from J. Messing,
            Waksman    Institute, NJ on floppy disk.    Revised 16-DEC-1986 by
            F. Pfeiffer:    1062/3 'AT' to 'TA' to match revised sequence of
            PBR322    The beta-galactosidase mRNA sequence including the
            multiple    cloning site of M13mp19 is on the strand complementary
            to that    shown. KEYWORDS CROSSREFERENCE    #complement
            VecBase(3):pUC19    #prerevised      GenBank(50):M11662,
            EMBL(11):ARPuc19    #parent      VecBase(3):pUC13,
            VecBase(3):M13mp19, VecSource(3):bGal19 PARENT    Features of
            pUC19c (2686 bp)      residue     source       1- 137  2074-2210
            pBR322     138- 237  2252-2351 pBR322     238- 395  1461-1304 (c)
            Lac-Operon     396- 452    57-   1 (c) polylinker of M13mp19
            455- 682  1298-1071 (c) Lac-Operon     683-2686  2352-4355 pBR322
            Conflict (cfl) and Mutations (mut):         pUC19c  source    mut
            1308  A  G  2977   pBR322   linked to increased copy number    mut
            1942  A  G  3611   pBR322    mut 2243  T  C  3912   pBR322 FEATURE
            1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER
            HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION
            #resistance  Ap    #indicator beta-galactosidase SUMMARY  pUC19c
            #length 2686   #checksum 4465.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Cloning vector pUC19c"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:174689"
                     /label=source:Cloning vector pUC19c
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          488..2733
                     /organism="Cloning vector pUC19c"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:174689"
                     /label=source:Cloning vector pUC19c(1)
                     /ApEinfo_label=source:Cloning vector pUC19c
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtGAATT Cacgcgcata tatatataat
      421 tatagagagt accgatgatg agcagcagaa tagatcagta cgctacgatg atagtagatg
      481 aCTGCAGgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1321 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1621 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1681 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1741 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1801 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1861 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1921 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1981 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2041 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2101 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2161 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2221 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2281 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2341 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2401 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2461 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2521 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2581 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2641 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2701 ataaaaatag gcgtatcacg aggccctttc gtc  
//
