Data File  : C:\Users\Public\Documents\ChemStation\2\Data\LC BO\0_2025-03-15 19-14-35.D
Acq. Method: GENERAL-POROSHELL-OPT.M


The Acq. Method's Instrument Parameters for the Run were : 


=====================================================================
                             HiP Sampler
=====================================================================

HiP Sampler (G4226A)
====================


Auxiliary                     
Draw Speed:                                     200.0 µL/min
Eject Speed:                                    200.0 µL/min
Draw Position Offset:                                 0.0 mm
Wait Time After Drawing:                               2.0 s
Sample Flush Out Factor:                                 5.0
Vial/Well bottom sensing:                                 No

Injection                     
Injection Mode:                   Injection with needle wash
Injection Volume:                                    0.35 µL

Needle Wash                   
Needle Wash Location:                             Flush Port
Wash Time:                                             3.0 s

High throughput               
Automatic Delay Volume Reduction:                         No

Overlapped Injection          
Enable Overlapped Injection:                              No

Valve Switching               
Valve Movements:                                           0

Valve Switch Time 1           
Switch Time 1 Enabled:                                    No

Valve Switch Time 2           
Switch Time 2 Enabled:                                    No

Valve Switch Time 3           
Switch Time 3 Enabled:                                    No

Valve Switch Time 4           
Switch Time 4 Enabled:                                    No

Stoptime                      
Stoptime Mode:                              As Pump/No Limit

Posttime                      
Posttime Mode:                                           Off



HiP Sampler (G4226A)
====================

Function            Parameter                               
------------------------------------------------------------
Draw                Draw 0.35 µL from location "P1-A4" with 
                    default speed  using default offset     
Inject              Inject                                  









=====================================================================
                             Column Comp.
=====================================================================

Column Comp. (G1316C)
=====================

Valve Position:                    Position 2 (Port 1 -> 10)
Ready when front door open:                              Yes

Left Temperature Control      
Temperature Control Mode:                     Not Controlled

Enable Analysis Left Temperature
Enable Analysis Left Temperature On:                     Yes
Enable Analysis Left Temperature Value:               0.8 °C

Right Temperature Control     
Right temperature Control Mode:                     Combined

Enable Analysis Right Temperature
Enable Analysis Right Temperature On:                    Yes
Enable Analysis Right Temperature Value:              0.8 °C

Stoptime                      
Stoptime Mode:                              As Pump/Injector

Posttime                      
Posttime Mode:                                           Off

Timetable                     




Instrument Curves
Store Left Temperature:                                    No
Store Right Temperature:                                   No






=====================================================================
                             Binary Pump
=====================================================================

Binary Pump (G4220A)
====================

Flow:                                           0.650 mL/min
Use Solvent Types:                                        No
Stroke Mode:                                    Synchronized
Low Pressure Limit:                                 0.00 bar
High Pressure Limit:                             1000.00 bar
Max. Flow Ramp Up:                           100.000 mL/min²
Max. Flow Ramp Down:                         100.000 mL/min²
Expected Mixer:                                     No check

Stroke A                      
Automatic Stroke Calculation A:                          Yes

Compress A                    
Compressibility Mode A:            Compressibility Value Set
Compressibility A:                              50 10e-6/bar

Compress B                    
Compressibility Mode B:            Compressibility Value Set
Compressibility B:                             115 10e-6/bar

Stoptime                      
Stoptime Mode:                                      Time set
Stoptime:                                           9.96 min

Posttime                      
Posttime Mode:                                      Time set
Posttime:                                           1.00 min
Solvent Composition
   Channel                           Name 1   Name 2
                                                    
----------------------------------------------------
         A        Water (+0.1% Formic Acid)         
         B Acetonitrile (+0.1% Formic Acid)         


   Selected   Used   Percent
                           %
----------------------------
      Ch. 1    Yes     95.00
      Ch. 1    Yes      5.00



Timetable
         Time     A      B   Flow   Pressure
          min     %      % mL/min        bar
--------------------------------------------
 Start. Cond. 95.00   5.00  0.650    1000.00
         9.69  0.00 100.00    ---        ---






Instrument Curves
Store Pressure:                                           Yes
Store Flow:                                               Yes
Store Solvent Ratio A:                                    Yes
Store Solvent Ratio B:                                    Yes
Store Tuning A:                                           Yes
Store Tuning B:                                           Yes






=====================================================================
                                 DAD
=====================================================================

DAD (G1315D)
============

UV Lamp Required:                                        Yes
Vis Lamp Required:                                        No
Peakwidth:        < 0.013 min (0.13 s response time) (20 Hz)
Slit:                                                   4 nm

Stoptime                      
Stoptime Mode:                              As Pump/Injector

Posttime                      
Posttime Mode:                                           Off

Analog Output 1               
Analog 1 Zero Offset:                                    5 %
Analog 1 Attenuation:                               1000 mAU

Analog Output 2               
Analog 2 Zero Offset:                                    5 %
Analog 2 Attenuation:                               1000 mAU

Signals                       
Signal table
   Acquire   Signal   Wavelength   Bandwidth   Use Ref.
                              nm          nm           
-------------------------------------------------------
       Yes Signal A          210           4         No
       Yes Signal B          230           4         No
       Yes Signal C          254           4         No
       Yes Signal D          310           4         No
       Yes Signal E          270           4         No
        No Signal F                                    
        No Signal G                                    
        No Signal H                                    




Prepare Mode                  
Margin for negative Absorbance:                      100 mAU

Autobalance                   
Autobalance Postrun:                                      No
Autobalance Prerun:                                      Yes

Spectrum                      
Spectrum Store:                                          All
Spectrum Range WL from:                               190 nm
Spectrum Range WL to:                                 400 nm
Spectrum Step:                                        2.0 nm

Timetable                     




Instrument Curves
Store Board Temperature:                                   No
Store Optical Unit Temperature:                            No
Store UV Lamp Anode Voltage:                               No








