Metadata-Version: 2.1
Name: primers
Version: 0.0.2
Summary: Create PCR primers optimized for length, tm, gc and free energy
Home-page: https://github.com/Lattice-Automation/primers
Author: JJTimmons
Author-email: jtimmons@latticeautomation.com
License: mit
Platform: UNKNOWN
Classifier: Development Status :: 3 - Alpha
Classifier: Programming Language :: Python :: 3 :: Only
Classifier: Topic :: Scientific/Engineering :: Bio-Informatics
Classifier: Environment :: Console
Requires-Python: >=3.0
Description-Content-Type: text/markdown
Requires-Dist: seqfold

# primers

```python
from primers import pcr, Primer

# create a FWD and REV primer
ps = pcr("ATGGATGGTAGAGATAGATGG",
    add_fwd="GGTAGGTAGAT", add_rev="GGTTTTAGGATAGAT", add_min=10, add_max=25,
    opt_tm=55.0, opt_len=30)

ps[0] # Primer("GGTAGGTAGATATGGATGGT", tm=62.3)
ps[1] # Primer("GGTTTTAGGATAGATCCTACTATCT", tm=64.0)
```


