sample: HL60 S/4 cell
medium: phosphate buffered saline (PBS, n=1.335)
imaging wavelength: 647nm
pixel size: 0.139µm
imaging techniuqe: shearing interferometry (SID4Bio, Phasics S.A.)
date of acquisition: 2016/12/07
file format: qpimage 0.2.0 (http://qpimage.readthedocs.io)

context:
These data were used to illustrate the advantages of combining
fluorescence tomography with optical diffraction tomography.
In the original strudy, the cell nucleus was segmented using the
fluorescence channel which enabled a quantification of its refractive
index (figures 2 and 4):

  M. Schürmann, G. Cojoc, S. Girardo, E. Ulbricht, J. Guck,
  and P. Müller,
  "Three-dimensional correlative single-cell imaging utilizing
  fluorescence and refractive index tomography,"
  Journal of Biophotonics 11(3): e201700145, 2017.
  doi:10.1002/jbio.201700145

The data are already background-corrected and laterally aligned as
discussed in the manuscript. The rotational positions are stored
in 'angles.txt'. A 3D reconstruction shows that the nuclear
refractive index of this cell is lower than that of the cytoplasm.

The HL60 cells were a generous gift from Donald and Ada Olins
(University of New England).

license: CC0
These data are dedicated to the public domain. You can copy, modify,
or distribute them without asking permission.
