Metadata-Version: 2.1
Name: mmv-im2im
Version: 0.2.0
Summary: A python package for deep learing based image to image transformation
Home-page: https://github.com/MMV-Lab/mmv_im2im
Author: Jianxu Chen
Author-email: jianxuchen.ai@gmail.com
License: MIT license
Keywords: deep learning,microscopy image analysis,biomedical image analysis
Platform: UNKNOWN
Classifier: Development Status :: 3 - Alpha
Classifier: Intended Audience :: Developers
Classifier: License :: OSI Approved :: MIT License
Classifier: Natural Language :: English
Classifier: Programming Language :: Python :: 3.8
Classifier: Programming Language :: Python :: 3.9
Requires-Python: >=3.8
Description-Content-Type: text/markdown
Provides-Extra: setup
Provides-Extra: test
Provides-Extra: dev
Provides-Extra: quick
Provides-Extra: all
License-File: LICENSE

# MMV Im2Im Transformation

[![Build Status](https://github.com/MMV-Lab/mmv_im2im/workflows/Build%20Main/badge.svg)](https://github.com/MMV-Lab/mmv_im2im/actions)
[![Documentation](https://github.com/MMV-Lab/mmv_im2im/workflows/Documentation/badge.svg)](https://MMV-Lab.github.io/mmv_im2im/)

A generic python package for deep learning based image-to-image transformation in biomedical applications

(We are actively working on the documentation and tutorials. Submit a feature request if there is anything you need.)

---

## Overview

The overall package is designed with a generic image-to-image transformation framework, which could be directly used for semantic segmentation, instance segmentation, image restoration, image generation, labelfree prediction, staining transformation, etc.. The implementation takes advantage of the state-of-the-art ML engineering techniques for users to focus on researches without worrying about the engineering details. In our pre-print [arxiv link](https://arxiv.org/abs/2209.02498), we demonstrated the effectiveness of *MMV_Im2Im* in more than ten different biomedical problems/datasets. 

* For biomedical machine learning researchers, we hope this new package could serve as the starting point for their specific problems to stimulate new biomedical image analysis or machine learning methods. 
* For experimental biomedical researchers, we hope this work can provide a holistic view of the image-to-image transformation concept with diverse examples, so that deep learning based image-to-image transformation could be further integrated into the assay development process and permit new biomedical studies that can hardly be done only with traditional experimental methods


## Installation

We recommend to [create a new conda environment](https://docs.conda.io/projects/conda/en/latest/user-guide/tasks/manage-environments.html#creating-an-environment-with-commands) or [a virtual environment](https://docs.python.org/3/library/venv.html) with Python 3.9.

### basic installation

(for users only using this package, not planning to change any code or make any extension):

**Stable Release:** `pip install mmv_im2im`<br>
**Development Head:** `pip install git+https://github.com/MMV-Lab/mmv_im2im.git`

### build from source

(for users planning to extend the methods or improve the code):

```
git clone https://github.com/MMV-Lab/mmv_im2im.git
cd mmv_im2im
pip install -e .[all]
```

Note: The `-e` option is the so-called "editable" mode. This will allow code changes taking effect immediately.


## Quick start / Try out on a simple example

Here, we use a [3D labelfree prediction](https://www.allencell.org/label-free-determination.html#:~:text=The%20Label-Free%20Determination%20model%20can%20leverage%20the%20specificity,structures.%20How%20does%20the%20label-free%20determination%20model%20work%3F) as an example. (If you followed [basic installation](#basic-installation), you will need to install the `quilt3` package by `pip install quilt3`, in order to download the data programmatically.)

**step 1 (data preparation):**, we pull 100 examples of lamin B1 images and the corresponding brightfield images from the AllenCell quilt bucket by running the following in the command line. 

```bash
# Suppose the current working directory is the root of mmv_im2im
python  scripts/pull_labelfree_sample_data.py --download_path /path/to/save/the/downloaded/images/ --structure LMNB1 --num 100 
```

**step 2 (train):** Now, we can train a labelfree model like this:
```bash
# Suppose the current working directory is the root of mmv_im2im
run_im2im --config train_labelfree_3d  --data.data_path /path/to/save/the/downloaded/train
```

This will apply all the default settings to train the 3D labelfree model. The training will stop after 100 epochs, 

**step 3 (test):** 

Suppose you run the training under the root of mmv_im2im. Then you will find a folder `mmv_im2im/lightning_logs/checkpoints`, where you can find several trained model. Here, we test with the model after the full training `last.ckpt` (other models are intermediate results for debugging purpose) by running:

```bash
# Suppose the current working directory is the root of mmv_im2im
run_im2im --config inference_labelfree_3d --data.inference_input.dir /path/to/save/the/downloaded/holdout --data.inference_output.path /path/to/save/predictions/ --model.checkpoint lightning_logs/checkpoints/last.ckpt
```

## Walk-through Guide

We provide [a tutorial](tutorials/README.md) for users to understand how to enjoy both the simplicity and the full flexibility of the package. 


## API Documentation

For full package API (i.e., the technical details of each function), please visit [MMV-Lab.github.io/mmv_im2im](https://MMV-Lab.github.io/mmv_im2im).


## Development

See [CONTRIBUTING.md](CONTRIBUTING.md) for information related to developing the code.


**MIT license**



