Metadata-Version: 1.2
Name: itsxpress
Version: 1.6.1
Summary: Rapidly trim sequences down to their Internally Transcribed Spacer (ITS) regions
Home-page: http://github.com/usda-ars-gbru/itsxpress
Author: Adam R. Rivers
Author-email: adam.rivers@ars.usda.gov
License: License :: CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Description: ITSxpress: Software to rapidly trim  the Internally transcribed spacer (ITS) region of FASTQ files
        ==================================================================================================
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        .. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.1304349.svg
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        Author
        -------
        * Adam R. Rivers, US Department of Agriculture, Agricultural Research Service
        
        
        Introduction
        -------------
        
        The internally transcribed spacer region is a region between highly conserved the small
        subunit (SSU) of rRNA and the large subunit (LSU) of the rRNA. In Eukaryotes it contains
        the 5.8s genes and two variable length spacer regions. In amplicon sequencing studies it is
        common practice to trim off the conserved (SSU, 5,8S or LSU) regions. `Bengtsson-Palme
        et al. (2013)`_ published software the software package ITSx_ to do this.
        
        ITSxpress is designed to support the calling of exact sequence variants rather than OTUs_.
        This newer method of sequence error-correction requires quality score data from each
        sequence, so each input sequence must be trimmed. ITSXpress makes this possible by
        taking FASTQ data, de-replicating the sequences then identifying the start and stop
        sites using HMMSearch.  Results are parsed and the trimmed files are returned. The ITS 1,
        ITS2 or the entire ITS region including the 5.8s rRNA gene can be selected. ITSxpress
        uses the hmm model from ITSx so results are comparable.
        
        ITSxpress is also available as a `QIIME2 Plugin`_
        
        .. _`Bengtsson-Palme et al. (2013)`: https://doi.org/10.1111/2041-210X.12073
        .. _ITSx: http://microbiology.se/software/itsx/
        .. _OTUs: https://doi.org/10.1038/ismej.2017.119
        .. _`QIIME2 Plugin`: https://github.com/USDA-ARS-GBRU/q2_itsxpress
        
        
        Installation
        -------------
        ITSxpress can be installed from:
        
        1. Bioconda: (preferred method because it handles dependencies):
        
        .. code-block:: bash
        
            conda install itsxpress
        
        2. Pip: https://pypi.org/project/itsxpress/:
        
        .. code-block:: bash
        
            pip install itsxpress
        
        
        3. The Github repository: https://github.com/USDA-ARS-GBRU/itsxpress
        
        .. code-block:: bash
        
            git clone https://github.com/USDA-ARS-GBRU/itsxpress.git
        
        
        Dependencies
        -------------
        The software requires Vsearch, BBtools, Hmmer >= 3.1b and Biopython. Bioconda
        takes care of this for you so it is the preferred installation method.
        
        
        Usage
        ---------
        
        -h, --help            	Show this help message and exit.
        
        --fastq 				A ``.fastq``, ``.fq``, ``.fastq.gz`` or ``.fq.gz`` file. Interleaved
                                	or not. Required.
        
        --single_end 			A flag to specify that the fastq file is single-ended (not paired).
                                	single-ended (not paired). Default is false.
        
        --fastq2 				A ``.fastq``, ``.fq``, ``.fastq.gz`` or ``.fq.gz`` file representing read 2 if present, optional.
        
        --outfile				The trimmed FASTQ file, if it ends in ``gz`` it will be gzipped.
        
        --tempdir				Specify the temp file directory. Default is None.
        
        --keeptemp				Should intermediate files be kept? Default is false.
        
        --region 				Options : {ITS2, ITS1, ALL}
        
        --taxa					Select the taxonomic group sequenced: {Alveolata, Bryophyta,
        						Bacillariophyta, Amoebozoa, Euglenozoa, Fungi, Chlorophyta,
        						Rhodophyta, Phaeophyceae, Marchantiophyta, Metazoa,
        						Oomycota, Haptophyceae, Raphidophyceae, Rhizaria, Synurophyceae,
        						Tracheophyta, Eustigmatophyceae, All}. Default Fungi.
        
        --cluster_id            The percent identity for clustering reads range [0.98-1.0], set to 1
                                for exact dereplication. Default 0.995.
        
        --log		          	Log file. Default is ITSxpress.log.
        
        --threads		     	Number of processor threads to use. Default is 1.
        
        
        Examples
        ---------
        
        Use case 1: Trimming the ITS2 region from a fungal amplicon sequencing dataset with
        forward and reverse gzipped FASTQ files using two cpu threads.
        
        .. code-block:: bash
        
            itsxpress --fastq r1.fastq.gz --fastq2 r2.fastq.gz --region ITS2 \
            --taxa Fungi --log logfile.txt --outfile trimmed_reads.fastq.gz --threads 2
        
        ITSxpress can take gzipped or un-gzipped FASTQ files and it can write gzipped or
        un-gzipped FASTQ files. It expects FASTQ files to end in: .fq, .fastq, .fq.gz or fastq.gz.
        
        
        Use case 2: Trimming the ITS2 region from a fungal amplicon sequencing dataset with
        an interleaved gzipped FASTQ files using two cpu threads.
        
        .. code-block:: bash
        
            itsxpress --fastq interleaved.fastq.gz  --region ITS2 --taxa Fungi \
            --log logfile.txt --outfile trimmed_reads.fastq.gz --threads 2
        
        
        Use case 3: Trimming the ITS2 region from a fungal amplicon sequencing dataset with
        an single-ended gzipped FASTQ files using two cpu threads.
        
        .. code-block:: bash
        
            itsxpress --fastq single-end.fastq.gz --single_end --region ITS2 --taxa Fungi \
            --log logfile.txt --outfile trimmed_reads.fastq.gz --threads 2
        
        Single ended data is less common and may come from a dataset where the reads have already
        been merged.
        
        Use case 4: Trimming the ITS1 region from a Alveolata amplicon sequencing dataset with
        an interleaved gzipped FASTQ files using 40 cpu threads.
        
        .. code-block:: bash
        
            itsxpress --fastq interleaved.fastq.gz --region ITS1 --taxa Alveolata \
            --log logfile.txt --outfile trimmed_reads.fastq.gz --threads 40
        
        
        License information
        --------------------
        
        This software is a work of the United States Department of Agriculture, Agricultural
        Research Service. 17 U.S.C. Section 105 states that "Copyright protection under this
        title is not available for any work of the United States Government".  While I anticipate
        that this work will be released under a CC0 public domain attribution, only the USDA ARS
        Office of Technology transfer has the authority to make that determination.
        
Keywords: Amplicon sequencing fungal ITS
Platform: UNKNOWN
Classifier: Topic :: Scientific/Engineering :: Bio-Informatics
Classifier: Programming Language :: Python :: 3.6
Classifier: Programming Language :: Python :: 3.5
Classifier: Development Status :: 3 - Alpha
Requires-Python: >3.5
