Metadata-Version: 2.1
Name: epimuller
Version: 0.0.6
Summary: Visualize lineages overtime, with phylogentic context, based on viral genomes
Home-page: https://github.com/jennifer-bio/epimuller
Author: Jennifer L Havens
Author-email: jhavens@ucsd.edu
License: MIT license
Description: # epiMuller README
        
        ![Muller plot image](https://raw.githubusercontent.com/jennifer-bio/epiMuller/main/images/case_scaled_lineages_long.png)
        
        ##### About
        
        ###### Author
        Jennifer L. Havens
        
        ###### Purpose
        Visualize lineages overtime, with phylogentic context, based on viral genomes.
        
        ###### Language
        Python3
        
        ###### Inputs
        Aligned viral genomes, collection dates, PANGO lineages, Nextstain JSON file, and timetree
        
        ###### Workflow overview
        
        - **epimuller-parse** (optional): parse fasta names with '*bar* isodate' suffix into usable fasta and metadata files.
        - **epimuller**: wrapper for epimuller-define and epimuller-draw.
        	 - **epimuller-define**: assigns samples to clades based on ancestral reconstruction of specified aa mutations or trait (hierarchy), and counts number of samples in a clade withen each time frame (abundance).
        	 - **epimuller-draw**: plots the frequency clades overtime, as specifed by abundance and hierarchy inputs from epimuller-define.
        
        ###### Source code avaliblity
        [gitHub](https://github.com/jennifer-bio/epimuller)
        
        ###### Documentation avaliblity 
        [Read the Docs](https://epimuller.readthedocs.io/en/stable/)
        
        ## Quick start
        
        ```
        pip3 install epimuller
        
        epimuller [-h] [-oDir OUTDIRECTORY] -oP OUTPREFIX
             (-n INNEXTSTRAIN | -a ANNOTATEDTREE) -m
             INMETA [-p INPANGOLIN] [--noPangolin]
             [-f TRAITOFINTERSTFILE] [-g GENEBOUNDRY]
             [-k TRAITOFINTERSTKEY]
             [-mut VOCLIST [VOCLIST ...]] [-t TIMEWINDOW]
             [-s STARTDATE] [-e ENDDATE] [-mt MINTIME]
             [-min MINTOTALCOUNT] [-c CASES_NAME]
             [-l {date,time}] [-lp {Right,Max,Start,End}]
             [--WIDTH WIDTH] [--HEIGHT HEIGHT]
             [--LEGENDWIDTH LEGENDWIDTH] [--MARGIN MARGIN]
             [--FONTSIZE FONTSIZE]
             [--LABELSHIFT LABELSHIFT]
        
        ```
        
        ## SOME EXAMPLES 
        
        #### Examples for full run 
        To see steps used to prep files for these examples look at scripts/Example_CommandsFromScratch.txt on [gitHub](https://github.com/jennifer-bio/epimuller/blob/main/scripts/Example_CommandsFromScratch.txt).
        
        ##### Visulize default aa mutation list
        
        ```
        epimuller \
        	-n inputData/GISAID_NYCPHL_04_29/02_nextstrainResults \
        	-m inputData/GISAID_NYCPHL_04_29/gisaid_2021_04_30_00_rename.tsv \
        	-oDir 03_results_NYCPHL_April29 \
        	-oP 01_defaultAAList \
        	-c inputData/CITY_US-NY_NYC_outbreakinfo_epidemiology_data_2021-04-30.tsv
        ```
        
        ##### Visulize a trait: lineage 
        
        ```
        epimuller \
        	-n inputData/GISAID_NYCPHL_04_29/02_nextstrainResults \
        	-m inputData/GISAID_NYCPHL_04_29/gisaid_2021_04_30_00_rename.tsv \
        	-oDir 03_results_NYCPHL_April29 \
        	-oP 02_pangolin \
        	-c inputData/CITY_US-NY_NYC_outbreakinfo_epidemiology_data_2021-04-30.tsv \
        	--traitOfInterstFile traits.json \
        	--traitOfInterstKey lineage \
        	-lp Max \
        	-min 100 \
        ```
        
        
        ##### Visulize your own aa mutation list
        
        ```
        epimuller \
        	-n inputData/GISAID_NYCPHL_04_29/02_nextstrainResults \
        	-m inputData/GISAID_NYCPHL_04_29/gisaid_2021_04_30_00_rename.tsv \
        	-oDir 03_results_NYCPHL_April29 \
        	-oP 03_selectedAA \
        	-c inputData/CITY_US-NY_NYC_outbreakinfo_epidemiology_data_2021-04-30.tsv \
        	-mut 'SE484K' 'S*452*' \
        	-min 50 \ 
        	-mt 20
        ```
        
        ##### Visulize default aa mutation list with TreeTime input
        
        ```
        epimuller \
        	-a inputData/GISAID_NYCPHL_04_29/06_treetimeDates_aa/timetree.nexus \
        	-oDir 04_results_NYCPHL_April29 \
        	-oP defaultAA_treetime \
        	-m inputData/GISAID_NYCPHL_04_29/gisaid_2021_04_30_00_rename.tsv \
        	-g data/geneAAboundries.json \
        	--FONTSIZE 18
        ```
        
        ##### Visulize a trait: lineage with TreeTime input
        
        ```
        epimuller \
        	-a inputData/GISAID_NYCPHL_04_29/06_treetimeDates_aa/timetree.nexus \
        	-oDir 03_results_NYCPHL_April29 \
        	-oP 05_pangolin_treetime \
        	-m inputData/GISAID_NYCPHL_04_29/gisaid_2021_04_30_00_rename.tsv \
        	--traitOfInterstKey lineage \
        	--noPangolin #do not label with mode of pangolin lineages in clade, label clade with defining lineage only 
        ```
        
        
        ## Known edge cases / featrues to add  
        Known edge cases which are not correctly dealt with or features I intend to address (eventually).
        If you run into anything else please let me know with an issue on [gitHub](https://github.com/jennifer-bio/epimuller/issues).
        ```
        	- feel free to ignore the undefined.svg that gets made - it is related to checking the size of the text to space out labels
        
        	- allow combination of aa mutants, not just 1
        	- define polytomy behavior
        ```
        
        ## Addtional features
        
        ##### Color
        If you would like to specify color for clade: in --parentHierarchy_name file (of epimuller-draw/drawMuller.py input) add col with name: "color" and hex color value (starting with #) for clades you want to specify.
        
        ##### Parse GISAID fasta for metadata
        epimuller-parse
        If you have downloaded sequences from GISAID under the search tab, you can parse out the names into a metadata file (format tested as of 2021-04-30).
        
        
        ## epimuller arguments  
        
        ```
        epimuller [-h] [-oDir OUTDIRECTORY] -oP OUTPREFIX
                 (-n INNEXTSTRAIN | -a ANNOTATEDTREE) -m
                 INMETA [-p INPANGOLIN] [--noPangolin]
                 [-f TRAITOFINTERSTFILE] [-g GENEBOUNDRY]
                 [-k TRAITOFINTERSTKEY]
                 [-mut VOCLIST [VOCLIST ...]] [-t TIMEWINDOW]
                 [-s STARTDATE] [-e ENDDATE] [-mt MINTIME]
                 [-min MINTOTALCOUNT] [-c CASES_NAME]
                 [-l {date,time}] [-lp {Right,Max,Start,End}]
                 [--WIDTH WIDTH] [--HEIGHT HEIGHT]
                 [--LEGENDWIDTH LEGENDWIDTH] [--MARGIN MARGIN]
                 [--FONTSIZE FONTSIZE]
                 [--LABELSHIFT LABELSHIFT]
        
        optional arguments:
          -h, --help            show this help message and exit
          -n INNEXTSTRAIN, --inNextstrain INNEXTSTRAIN
        		nextstrain results with tree.nwk and
        		[traitOfInterst].json (default: None)
          -a ANNOTATEDTREE, --annotatedTree ANNOTATEDTREE
        		nexus file name and [traitOfInterst].json (default:
        		None)
        
        Options for full repot:
          -oDir OUTDIRECTORY, --outDirectory OUTDIRECTORY
        		folder for output (default: ./)
          -oP OUTPREFIX, --outPrefix OUTPREFIX
        		prefix of out files withen outDirectory (default:
        		None)
        
        Options passed to epimuller-define:
          -m INMETA, --inMeta INMETA
        		metadata tsv with 'strain' and 'date'cols, optional:
        		cols of trait of interst; and pangolin col named:
        		'lineage' or 'pangolin_lin' (default: None)
          -p INPANGOLIN, --inPangolin INPANGOLIN
        		pangolin output lineage_report.csv file, if argument
        		not supplied looks in inMeta for col with
        		'pangolin_lin' or 'lineage' (default: metadata)
          --noPangolin          do not add lineage to cade names (default: False)
          -f TRAITOFINTERSTFILE, --traitOfInterstFile TRAITOFINTERSTFILE
        		name of nextstrain [traitOfInterst].json in
        		'inNextstrain' folder (default: aa_muts.json)
          -g GENEBOUNDRY, --geneBoundry GENEBOUNDRY
        		json formated file specifing start end postions of
        		genes in alnment for annotatedTree with aa_muts option
        		(default: None)
          -k TRAITOFINTERSTKEY, --traitOfInterstKey TRAITOFINTERSTKEY
        		key for trait of interst in json file or annotated
        		tree file for aa with 'mutations' annotation, use
        		'aa_muts' (default: aa_muts)
          -mut VOCLIST [VOCLIST ...], --VOClist VOCLIST [VOCLIST ...]
        		list of aa of interest in form
        		[GENE][*ORAncAA][site][*ORtoAA] ex. S*501*, gaps
        		represed by X (default: None)
          -t TIMEWINDOW, --timeWindow TIMEWINDOW
        		number of days for sampling window (default: 7)
          -s STARTDATE, --startDate STARTDATE
        		start date in iso format YYYY-MM-DD or 'firstDate'
        		which sets start date to first date in metadata
        		(default: 2020-03-01)
          -e ENDDATE, --endDate ENDDATE
        		end date in iso format YYYY-MM-DD or 'lastDate' which
        		sets end date as last date in metadata (default:
        		lastDate)
        
        Options passed to epimuller-draw:
          -mt MINTIME, --MINTIME MINTIME
        		minimum time point to start plotting (default: 30)
          -min MINTOTALCOUNT, --MINTOTALCOUNT MINTOTALCOUNT
        		minimum total count for group to be included (default:
        		50)
          -c CASES_NAME, --cases_name CASES_NAME
        		file with cases - formated with 'date' in ISO format
        		and 'confirmed_rolling' cases, in tsv format (default:
        		None)
          -l {date,time}, --xlabel {date,time}
        		Format of x axis label: ISO date format or timepoints
        		from start (default: date)
          -lp {Right,Max,Start,End}, --labelPosition {Right,Max,Start,End}
        		choose position of clade labels (default: Right)
        
        Options passed to epimuller-draw for page setup:
          --WIDTH WIDTH         WIDTH of page (px) (default: 1500)
          --HEIGHT HEIGHT       HEIGHT of page (px) (default: 1000)
          --LEGENDWIDTH LEGENDWIDTH
        		LEGENDWIDTH to the right of plotting area (px)
        		(default: 220)
          --MARGIN MARGIN       MARGIN around all sides of plotting area (px)
        		(default: 60)
          --FONTSIZE FONTSIZE
          --LABELSHIFT LABELSHIFT
        
        ```
        
        
        
        ## epimuller-define: make abundance and hiearchy files 
        
        ```
        epimuller-define  [-h] (-n INNEXTSTRAIN | -a ANNOTATEDTREE) -m
               INMETA [-p INPANGOLIN] [--noPangolin]
               [-f TRAITOFINTERSTFILE] [-g GENEBOUNDRY]
               [-k TRAITOFINTERSTKEY]
               [-mut VOCLIST [VOCLIST ...]]
               [-oDir OUTDIRECTORY] -oP OUTPREFIX
               [-t TIMEWINDOW] [-s STARTDATE] [-e ENDDATE]
        
        optional arguments:
          -h, --help            show this help message and exit
          -n INNEXTSTRAIN, --inNextstrain INNEXTSTRAIN
        		nextstrain results with tree.nwk and
        		[traitOfInterst].json (default: None)
          -a ANNOTATEDTREE, --annotatedTree ANNOTATEDTREE
        		nexus file name (default: None)
          -m INMETA, --inMeta INMETA
        		metadata tsv with 'strain' and 'date'cols, optional:
        		cols of trait of interst; and pangolin col named:
        		'lineage' or 'pangolin_lin' (default: None)
          -p INPANGOLIN, --inPangolin INPANGOLIN
        		pangolin output lineage_report.csv file, if argument
        		not supplied looks in inMeta for col with
        		'pangolin_lin' or 'lineage' (default: metadata)
          --noPangolin          do not add lineage to cade names (default: False)
          -f TRAITOFINTERSTFILE, --traitOfInterstFile TRAITOFINTERSTFILE
        		name of nextstrain [traitOfInterst].json in
        		'inNextstrain' folder (default: aa_muts.json)
          -g GENEBOUNDRY, --geneBoundry GENEBOUNDRY
        		json formated file specifing start end postions of
        		genes in alnment for annotatedTree with aa_muts option
        		(default: None)
          -k TRAITOFINTERSTKEY, --traitOfInterstKey TRAITOFINTERSTKEY
        		key for trait of interst in json file or annotated
        		tree file for aa with 'mutations' annotation, use
        		'aa_muts', see example data/geneAAboundries.json
        		(default: aa_muts)
          -mut VOCLIST [VOCLIST ...], --VOClist VOCLIST [VOCLIST ...]
        		list of aa of interest in form
        		[GENE][*ORAncAA][site][*ORtoAA] ex. S*501*, gaps
        		represed by X (default: None)
          -oDir OUTDIRECTORY, --outDirectory OUTDIRECTORY
        		folder for output (default: ./)
          -oP OUTPREFIX, --outPrefix OUTPREFIX
        		prefix of out files withen outDirectory (default:
        		None)
          -t TIMEWINDOW, --timeWindow TIMEWINDOW
        		number of days for sampling window (default: 7)
          -s STARTDATE, --startDate STARTDATE
        		start date in iso format YYYY-MM-DD or 'firstDate'
        		which is in metadata (default: 2020-03-01)
          -e ENDDATE, --endDate ENDDATE
        		end date in iso format YYYY-MM-DD or 'lastDate' which
        		is in metadata (default: lastDate)
        ```
        
        
        ## epimuller-draw: plot
        
        ```
        epimuller-draw [-h] -p PARENTHIERARCHY_NAME -a ABUNDANCE_NAME
        		[-c CASES_NAME] -o OUTFOLDER [-mt MINTIME]
        		[-min MINTOTALCOUNT] [-l {date,time}]
        		[-lp {Right,Max,Start,End}] [--WIDTH WIDTH]
        		[--HEIGHT HEIGHT] [--LEGENDWIDTH LEGENDWIDTH]
        		[--LABELSHIFT LABELSHIFT] [--MARGIN MARGIN]
        		[--FONTSIZE FONTSIZE]
        
        optional arguments:
          -h, --help            show this help message and exit
          -p PARENTHIERARCHY_NAME, --parentHierarchy_name PARENTHIERARCHY_NAME
        		csv output from mutationLinages_report.py with child
        		parent col (default: None)
          -a ABUNDANCE_NAME, --abundance_name ABUNDANCE_NAME
        		csv output from mutationLinages_report.py with
        		abundances of clades (default: None)
          -c CASES_NAME, --cases_name CASES_NAME
        		file with cases - formated with 'date' in ISO format
        		and 'confirmed_rolling' cases, in tsv format (default:
        		None)
          -o OUTFOLDER, --outFolder OUTFOLDER
        		csv output from mutationLinages_report.py with child
        		parent col (default: None)
          -mt MINTIME, --MINTIME MINTIME
        		minimum time point to start plotting (default: 30)
          -min MINTOTALCOUNT, --MINTOTALCOUNT MINTOTALCOUNT
        		minimum total count for group to be included (default:
        		50)
          -l {date,time}, --xlabel {date,time}
        		Format of x axis label: ISO date format or timepoints
        		from start (default: date)
          -lp {Right,Max,Start,End}, --labelPosition {Right,Max,Start,End}
        		choose position of clade labels (default: Right)
        
        Options for page setup:
          --WIDTH WIDTH         WIDTH of page (px) (default: 1500)
          --HEIGHT HEIGHT       HEIGHT of page (px) (default: 1000)
          --LEGENDWIDTH LEGENDWIDTH
        		LEGENDWIDTH to the right of plotting area (px)
        		(default: 220)
          --LABELSHIFT LABELSHIFT
        		nudge label over by LABELSHIFT (px) (default: 15)
          --MARGIN MARGIN       MARGIN around all sides of plotting area (px)
        		(default: 60)
          --FONTSIZE FONTSIZE
        ```
        
        ## Install methods
        
        ##### With Bioconda
        
        ```
        conda install -c bioconda epimuller
        ```
        
        ##### With pip
        
        ```
        pip3 install epimuller
        
        #If there is an issue with cairo, try:
        
        pip3 install pycairo
        pip3 install epimuller
        ```
        
        ##### From source
        
        Download source code from [gitHub](https://github.com/jennifer-bio/epimuller) or [pypi](https://pypi.org/project/epimuller/)
        
        ```
        #open as needed for download format
        tar -zxvf epimuller-[version].tar.gz
        
        cd epimuller-[version]
        
        python3 setup.py install
        ```
        
        ##### Run scripts directly
        This is currently the best way to change display and font size, howerver you will have to install all dependencies.
        
        Download source code from [gitHub](https://github.com/jennifer-bio/epimuller) or [pypi](https://pypi.org/project/epimuller/)
        
        ```
        #open as needed for download format
        tar -zxvf epimuller-[version].tar.gz
        
        cd epimuller-[version]
        
        #to run epimuller
        python3 ./scripts/mutationLinages_report.py [arugments]
        
        #to run epimuller-parse
        python3 ./scripts/parseFastaNames.py  [arugments]
        
        #to run epimuller-define 
        python3 ./scripts/defineAndCountClades.py  [arugments]
        
        #to run epimuller-draw 
        python3 ./scripts/drawMuller.py  [arugments]
        
        ```
        
        
        ## Citation
        
        Please [link to this github](https://github.com/jennifer-bio/epimuller) if you have used epimuller in your research. 
        
        #### Extra notes on GISAID
        
        If you do use GISAID data please acknowledge the contributers, such as with [language suggested by GISAID](https://www.gisaid.org/help/publish-with-data-from-gisaid/).
Keywords: epimuller
Platform: UNKNOWN
Classifier: Development Status :: 2 - Pre-Alpha
Classifier: Intended Audience :: Science/Research
Classifier: Intended Audience :: Healthcare Industry
Classifier: License :: OSI Approved :: MIT License
Classifier: Natural Language :: English
Classifier: Programming Language :: Python :: 3.7
Classifier: Programming Language :: Python :: 3.8
Classifier: Programming Language :: Python :: 3.9
Classifier: Topic :: Scientific/Engineering :: Bio-Informatics
Classifier: Topic :: Scientific/Engineering :: Visualization
Requires-Python: >=3.7
Description-Content-Type: text/markdown
