Metadata-Version: 2.4
Name: dropbucket
Version: 0.1.0
Summary: Robust SNP-based demultiplexing tool
Author-email: dongjuleem <ehdwn5170@g.skku.edu>
Maintainer-email: dongjuleem <ehdwn5170@g.skku.edu>
License: MIT
Requires-Python: >=3.9
Description-Content-Type: text/markdown
License-File: LICENSE
Requires-Dist: numpy==1.26.4
Requires-Dist: pandas==2.2.3
Requires-Dist: scipy==1.13.1
Requires-Dist: scikit-learn==1.6.1
Dynamic: license-file

### dropbucket
Robust SNP-based demultiplexing tool designed to accurately assign cells to donors in pooled scRNA-seq datasets, even under extremely unbalanced pooling condition and samples with genetically similar genotypes.

### Usage
```bash
   python dropbucket_pipeline.py \
     --mtx_path dir/mtx_path \
     --cellbarcode_path dir/barcodes.tsv \
     --k 8 \
     --output_dir results
```

### Requirements (Python)

- Python >= 3.9
- numpy
- pandas
- scipy
- scikit-learn

### Requirements (External tools)

- freebayes
- vartrix
