LOCUS               pGP4G_EYFP        5606 bp ds-DNA   circular     04-OCT-2013
DEFINITION  pAG304GPD-EYFP-ccdB Sequence
ACCESSION    
KEYWORDS    
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(29..51)
                     /label=pGEX_3_primer
                     /ApEinfo_fwdcolor=#9384ff
                     /ApEinfo_revcolor=#00ff29
     misc_feature    2918..3372
                     /label=TRP1 3' UTR extended
                     /ApEinfo_fwdcolor=#b38bff
                     /ApEinfo_revcolor=green
     misc_feature    337..346
                     /label=Fill-in removed EcoRI
                     /ApEinfo_fwdcolor=#a17689
                     /ApEinfo_revcolor=green
     misc_feature    1119..1164
                     /label=TRP1 3' UTR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    444..1118
                     /label=TRP1
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    193..443
                     /label=TRP1 5' UTR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     rep_origin      complement(3772..4391)
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    complement(2653..2669)
                     /label=pBluescriptKS_primer
                     /ApEinfo_fwdcolor=#b390ff
                     /ApEinfo_revcolor=#00ff29
     misc_feature    2527..2548
                     /label=EGFP_C_primer
                     /ApEinfo_fwdcolor=#9384ff
                     /ApEinfo_revcolor=#00ff29
     CDS             complement(4546..5406)
                     /gene="Ampicillin"
                     /note="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label=Ampicillin
                     /ApEinfo_fwdcolor=#ffff01
                     /ApEinfo_revcolor=green
     misc_feature    1708..1731
                     /label=GPDPro_F_primer
                     /ApEinfo_fwdcolor=#9384ff
                     /ApEinfo_revcolor=#00ff29
     misc_feature    complement(3398..3420)
                     /label=M13_pUC_rev_primer
                     /ApEinfo_fwdcolor=#9384ff
                     /ApEinfo_revcolor=#00ff29
     misc_feature    complement(2687..2705)
                     /label=CYC1_primer
                     /ApEinfo_fwdcolor=#b390ff
                     /ApEinfo_revcolor=#00ff29
     gene            complement(4546..5406)
                     /gene="Ampicillin"
                     /label=Ampicillin(1)
                     /ApEinfo_label=Ampicillin
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=pink
     misc_feature    complement(2687..2705)
                     /label=pYESTrp_rev_primer
                     /ApEinfo_fwdcolor=#b390ff
                     /ApEinfo_revcolor=#00ff29
     misc_feature    2591..2593
                     /label=Stop Codon
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     promoter        complement(5448..5476)
                     /label=AmpR_promoter
                     /ApEinfo_fwdcolor=#602ef3
                     /ApEinfo_revcolor=pink
     misc_feature    1869..1870
                     /label=TC In Frame
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(1919..1940)
                     /label=EGFP_N_primer
                     /ApEinfo_fwdcolor=#9384ff
                     /ApEinfo_revcolor=#00ff29
     terminator      2678..2917
                     /label=CYC1_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     CDS             complement(1874..2590)
                     /translation="MSTTWSTLVQLVHAESDPGGGHELQQDHVIALLVGVFAQGGLVA
                     QVVVVGQQHGAVADGGVLLVVVGELHAAVLDVVADLEVHLDAVLLLVGHDIDVVAVVV
                     VLQLVPQDVAVLLEVDALQLDAVHQGVALELHLGAGLVVAVVLEEDGALLDVAFGHGG
                     LEEVVLLHVVGVAGEALQAVAEGGHEGGPGHGQLAGGADELQGQLAVGGIALALAGHA
                     ELVAVYVAVQLDQDGHHPGEQLLALAHHFVPGDPLVLESVETKFWCFKTKKKTNYKSR
                     I*"
                     /label=ORF frame 2
                     /ApEinfo_fwdcolor=#ffff01
                     /ApEinfo_revcolor=green
     misc_feature    complement(3381..3399)
                     /label=M13_reverse_primer
                     /ApEinfo_fwdcolor=#9384ff
                     /ApEinfo_revcolor=#00ff29
     misc_feature    3373..3380
                     /label=PmeI site
                     /ApEinfo_fwdcolor=#6cff69
                     /ApEinfo_revcolor=green
     CDS             1874..2590
                     /gene="EYFP"
                     /note="ORF frame 2"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGPGGGHQTSLYKKAERET*"
                     /label=EYFP
                     /ApEinfo_fwdcolor=#ffff01
                     /ApEinfo_revcolor=green
     misc_feature    185..192
                     /label=PmeI site(1)
                     /ApEinfo_label=PmeI site
                     /ApEinfo_fwdcolor=#6cff69
                     /ApEinfo_revcolor=green
     misc_feature    1820..1836
                     /label=pBluescriptSK_primer
                     /ApEinfo_fwdcolor=#b390ff
                     /ApEinfo_revcolor=#00ff29
     misc_feature    1845..1868
                     /label=attB1
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
     misc_feature    2594..2618
                     /label=attB2
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
     misc_feature    1844..1844
                     /label=attR1
                     /ApEinfo_fwdcolor=#b390ff
                     /ApEinfo_revcolor=#00ff29
     misc_feature    1165..1819
                     /label=Actual GPD Promoter
                     /ApEinfo_fwdcolor=#009bff
                     /ApEinfo_revcolor=green
     misc_feature    1871..1873
                     /label=Yeast Kozak
                     /ApEinfo_fwdcolor=#000eff
                     /ApEinfo_revcolor=green
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaGTTTAA ACCATTTAAT AGAACAGCAT CGTAATATAT GTGTACTTTG CAGTTATGAC
      241 GCCAGATGGC AGTAGTGGAA GATATTCTTT ATTGAAAAAT AGCTTGTCAC CTTACGTACA
      301 ATCTTGATCC GGAGCTTTTC TTTTTTTGCC GATTAAgaat taattcGGTC GAAAAAAGAA
      361 AAGGAGAGGG CCAAGAGGGA GGGCATTGGT GACTATTGAG CACGTGAGTA TACGTGATTA
      421 AGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT TCTGGTCCAT
      481 TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA GATTCCGATG
      541 CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC CCGGTTATTG
      601 CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT CCGAAATACT
      661 TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC AATGATTACG
      721 GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA GAGTTCCTCG
      781 GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA CTCAGTGCAG
      841 CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT GGGACAGGTG
      901 AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC CCCGAAAGCT
      961 TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG CTTAGATTAA
     1021 ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA AAAGACTCTA
     1081 ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG TAGTATTTAT
     1141 TTAAGTATTG TTTGTGCACT TGCCagttta tcattatcaa tactcgccat ttcaaagaat
     1201 acgtaaataa ttaatagtag tgattttcct aactttattt agtcaaaaaa ttagcctttt
     1261 aattctgctg taacccgtac atgcccaaaa tagggggcgg gttacacaga atatataaca
     1321 tcgtaggtgt ctgggtgaac agtttattcc tggcatccac taaatataat ggagcccgct
     1381 ttttaagctg gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt tttcttcacc
     1441 aaccatcagt tcataggtcc attctcttag cgcaactaca gagaacaggg gcacaaacag
     1501 gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa atgatgacac
     1561 aaggcaattg acccacgcat gtatctatct cattttctta caccttctat taccttctgc
     1621 tctctctgat ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg aaattattcc
     1681 cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct gtaaatctat
     1741 ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt taaaacacca
     1801 gaacttagtt tcgacggatt ctagaactag tggatccccc atcaCAAGTT TGTACAAAAA
     1861 AGCAGGCTTC AAAatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1921 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1981 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2041 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
     2101 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2161 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2221 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2281 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2341 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2401 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2461 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     2521 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2581 gctgtacaag taaCACCCAG CTTTCTTGTA CAAAGTGGtg atgggctgca ggaattcgat
     2641 atcaagctta tcgataccgt cgacctcgag tcatgtaatt agttatgtca cgcttacatt
     2701 cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct
     2761 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa
     2821 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc
     2881 ttgagaaggt tttgggacgc tcgaaggctt taatttgTGC AGGCCTTTTG AAAAGCAAGC
     2941 ATAAAAGATC TAAACATAAA ATCTGTAAAA TAACAAGATG TAAAGATAAT GCTAAATCAT
     3001 TTGGCTTTTT GATTGATTGT ACAGGAAAAT ATACATCGCA GGGGGTTGAC TTTTACCATT
     3061 TCACCGCAAT GGAATCAAAC TTGTTGAAGA GAATGTTCAC AGGCGCATAC GCTACAATGA
     3121 CCCGATTCTT GCTAGCCTTT TCTCGGTCTT GCAAACAACC GCCGGCAGCT TAGTATATAA
     3181 ATACACATGT ACATACCTCT CTCCGTATCC TCGTAATCAT TTTCTTGTAT TTATCGTCTT
     3241 TTCGCTGTAA AAACTTTATC ACACTTATCT CAAATACACT TATTAACCGC TTTTACTATT
     3301 ATCTTCTACG CTGACAGTAA TATCAAACAG TGACACATAT TAAACACAGT GGTTTCTTTG
     3361 CATAAACACC ATGTTTAAAC catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3421 cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3481 agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3541 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3601 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3661 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3721 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3781 ggcgtttttc cataggctcg gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3841 gaggtggcga aacccgacag gactataaag ataccaggcg ttcccccctg gaagctccct
     3901 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3961 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4021 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4081 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4141 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4201 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4261 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4321 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4381 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4441 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4501 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4561 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactgcc
     4621 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4681 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4741 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4801 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4861 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4921 acgatcaagg cgagttacat gatcccccat gttgtgaaaa aaagcggtta gctccttcgg
     4981 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5041 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5101 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5161 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5221 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5281 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5341 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5401 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5461 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5521 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5581 taggcgtatc acgaggccct ttcgtc   
//
