LOCUS       pMODKan_HO_pACT1GE        7805 bp ds-DNA   circular     24-SEP-2013
DEFINITION  Leu2 plasmid with modular linkers for flanking funtional genetic
            units.
ACCESSION   urn.local...1326666445012.17
KEYWORDS    .
SOURCE      
  ORGANISM  . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    4666..5571
                     /label=upstream HO targeting
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     terminator      4404..4643
                     /created_by="User"
                     /label=CYC1
                     /ApEinfo_fwdcolor=#59c0cb
                     /ApEinfo_revcolor=pink
     misc_feature    4141..4344
                     /label=HSV1 VP16
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    756..1152
                     /label=KANMX Wach et al 1994 (genome del. project)
                     /ApEinfo_fwdcolor=#edd079
                     /ApEinfo_revcolor=#ffe28b
     misc_feature    3256..4140
                     /label=hER HBD
                     /ApEinfo_fwdcolor=#6c59ff
                     /ApEinfo_revcolor=green
     misc_feature    5890..6744
                     /label=ori
                     /ApEinfo_fwdcolor=#f1de49
                     /ApEinfo_revcolor=green
     misc_feature    2941..3218
                     /label=GAL4(1-93) DBD
                     /ApEinfo_fwdcolor=#00ff29
                     /ApEinfo_revcolor=green
     misc_feature    2225..2885
                     /label=pACT1
                     /ApEinfo_fwdcolor=#f6ff39
                     /ApEinfo_revcolor=green
     misc_feature    6745..7605
                     /label=bla
                     /ApEinfo_fwdcolor=#fcabe4
                     /ApEinfo_revcolor=green
     misc_feature    complement(29..51)
                     /label=pGEX_3_primer
                     /ApEinfo_fwdcolor=#96bbff
                     /ApEinfo_revcolor=cyan
     misc_feature    5580..5619
                     /label=M13R
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    5572..5579
                     /label=PmeI
                     /ApEinfo_fwdcolor=#96bbff
                     /ApEinfo_revcolor=cyan
     misc_feature    5805..5889
                     /label=origin-extended
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    7606..7684
                     /label=AmpR promoter
                     /ApEinfo_fwdcolor=#f96061
                     /ApEinfo_revcolor=green
     misc_feature    1153..1962
                     /label=KanMX CDS
                     /ApEinfo_fwdcolor=#acb1ff
                     /ApEinfo_revcolor=green
     misc_feature    1963..2200
                     /label=KanMX terminator
                     /ApEinfo_fwdcolor=#e9c021
                     /ApEinfo_revcolor=green
     primer_bind     2201..2224
                     /modified_by="User"
                     /label=M13 Forward (-47) primer
                     /ApEinfo_fwdcolor=#1ed628
                     /ApEinfo_revcolor=#63c05c
     primer_bind     4644..4665
                     /created_by="User"
                     /label=T7 EEV primer
                     /ApEinfo_fwdcolor=#6cc0cb
                     /ApEinfo_revcolor=#63c05c
     primer_bind     complement(4413..4431)
                     /created_by="User"
                     /label=pYESTrp_rev primer
                     /ApEinfo_fwdcolor=#6cc0cb
                     /ApEinfo_revcolor=#63c05c
     misc_feature    726..755
                     /label=pMOD_t1suf
                     /ApEinfo_fwdcolor=#0071ff
                     /ApEinfo_revcolor=green
     misc_feature    163..725
                     /label=HO Targeting 1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    155..162
                     /label=PmeI(1)
                     /ApEinfo_label=PmeI
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
     misc_feature    133..154
                     /label=pMOD_t1pre
                     /ApEinfo_fwdcolor=#0064ff
                     /ApEinfo_revcolor=green
     misc_feature    4348..4377
                     /label=L2
                     /ApEinfo_fwdcolor=#40ff40
                     /ApEinfo_revcolor=green
     misc_feature    4345..4347
                     /label=stop codon
                     /ApEinfo_fwdcolor=#d0410e
                     /ApEinfo_revcolor=green
     misc_feature    5572..5579
                     /label=PmeI site
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
     misc_feature    3219..3219
                     /label=Differs from Gottschling map (backbone)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2922..2932
                     /label=Extra sequence not found in Gottschling map
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    4378..4399
                     /label=T + pBluescript KS linker
                     /ApEinfo_fwdcolor=#eb66ff
                     /ApEinfo_revcolor=green
     misc_feature    4236..4236
                     /label=Differs from Gottschling Map
                     /ApEinfo_fwdcolor=#ff4638
                     /ApEinfo_revcolor=green
     misc_feature    7685..7704
                     /label=New Feature
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    6427..6427
                     /label=is a g in normal maps.
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcGTTTAA ACTTAGCAGA TGCGCGCACC
      181 TGCGTTGTTA CCACAACTCT TATGAGGCCC GCGGACAGCA TCAAActgta agattccgcc
      241 acattttata cactctggtc ctttaactgg caaaccttcg ggcgtaatgc ccaatttttc
      301 gcctttgtct tttgcctttt tcacttcacg tgcttctggt acatacttgc aatttataca
      361 gtgatgaccg ctgaatttgt atcttccata gcatctagca catactcgat ttttaccact
      421 ccaatcttta taaaaatact tgattccctt tctgggacaa gcaacacagt gttttagatt
      481 ctttttttgt gatattttaa gctgttctcc cacacagcag cctcgacatg atttcacttc
      541 tattttgttg ccaagcaaga aatttttatg gccttctatc gtaagcccat atacagtact
      601 ctcaccctgg aaatcatccg tgaagctgaa atatacgggt tcccttttta taattggcgg
      661 aacttctctt gttttgtgac cacttcgaca atatgacaaa acattctgtg aagttgttcc
      721 cccagcatca gagcagattg tactgagagt gcaccGGCGC GCCAGATCTG TTTAGCTTGC
      781 CTCGTCCCCG CCGGGTCACC CGGCCAGCGA CATGGGGGCC CAGAATACCC TCCTTGACAG
      841 TCTTGACGTG CGCAGCTCAG GGGCATGATG TGACTGTCGC CCGTACATTT AGCCCATACA
      901 TCCCCATGTA TAATCATTTG CATCCATACA TTTTGATGGC CGCACGGCGC GAAGCAAAAA
      961 TTACGGCTCC TCGCTGCAGA CCTGCGAGCA GGGAAACGCT CCCCTCACAG ACGCGTTGAA
     1021 TTGTCCCCAC GCCGCGCCCC TGTAGAGAAA TATAAAAGGT TAGGATTTGC CACTGAGGTT
     1081 CTTCTTTCAT ATACTTCCTT TTAAAATCTT GCTAGGATAC AGTTCTCACA TCACATCCGA
     1141 ACATAAACAA CCATGGGTAA GGAAAAGACT CACGTTTCGA GGCCGCGATT AAATTCCAAC
     1201 ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG
     1261 ACAATCTATC GATTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA
     1321 GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT
     1381 ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC
     1441 ACTGCGATCC CCGGCAAAAC AGCATTCCAG ATATTAGAAG AATATCCTGA TTCAGGTGAA
     1501 AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT
     1561 TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC
     1621 GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC
     1681 TGGAAAGAAA TGCATAAGCT TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT
     1741 TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA
     1801 CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG
     1861 TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG
     1921 AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGTACT GACAATAAAA
     1981 AGATTCTTGT TTTCAAGAAC TTGTCATTTG TATAGTTTTT TTATATTGTA GTTGTTCTAT
     2041 TTTAATCAAA TGTTAGCGTG ATTTATATTT TTTTTCGCCT CGACATCATC TGCCCAGATG
     2101 CGAAGTTAAG TGCGCAGAAA GTAATATCAT GCGTCAATCG TATGTGAATG CTGGTCGCTA
     2161 TACTGCTGTC GATTCGATAC TAACGCCGCC ATCCAGTGTC cgccagggtt ttcccagtca
     2221 cgacGCCTCT ACCTTGCAGA CCCATATAAT ATAATAACTA AATAAGTAAA TAAGACACAC
     2281 GCGAGAACAT ATATACACAA TTACAGTAAC AATAACAAGA GGACAGATAC TACCAAAATG
     2341 TGTGGGGAAG CGGGTAAGCT GCCACAGCAA TTAATGCACA ACATTTAACC TACATTCTTC
     2401 CTTATCGGAT CCTCAAAACC CTTAAAAACA TATGCCTCAC CCTAACATAT TTTCCAATTA
     2461 ACCCTCAATA TTTCTCTGTC ACCCGGCCTC TATTTTCCAT TTTCTTCTTT ACCCGCCACG
     2521 CGTTTTTTTC TTTCAAATTT TTTTCTTCCT TCTTCTTTTT CTTCCACGTC CTCTTGCATA
     2581 AATAAATAAA CCGTTTTGAA ACCAAACTCG CCTCTCTCTC TCCTTTTTGA AATATTTTTG
     2641 GGTTTGTTTG ATCCTTTCCT TCCCAATCTC TCTTGTTTAA TATATATTCA TTTATATCAC
     2701 GCTCTCTTTT TATCTTCCTT TTTTTCCTCT CTCTTGTATT CTTCCTTCCC CTTTCTACTC
     2761 AAACCAAGAA GAAAAAGAAA AGGTCAATCT TTGTTAAAGA ATAGGATCTT CTACTACATC
     2821 AGCTTTTAGA TTTTTCACGC TTACTGCTTT TTTCTTCCCA AGATCGAAAA TTTACTGAAT
     2881 TAACAgggcc ccccctcgag gtcgacggta tcgataAGCT tGAAGCAAGC CTcctgaaag
     2941 ATGAAGCTAC TGTCTTCTAT CGAACAAGCA TGCGATATTT GCCGACTTAA AAAGCTCAAG
     3001 TGCTCCAAAG AAAAACCGAA GTGCGCCAAG TGTCTGAAGA ACAACTGGGA GTGTCGCTAC
     3061 TCTCCCAAAA CCAAAAGGTC TCCGCTGACT AGGGCACATC TGACAGAAGT GGAATCAAGG
     3121 CTAGAAAGAC TGGAACAGCT ATTTCTACTG ATTTTTCCTC GAGAAGACCT TGACATGATT
     3181 TTGAAAATGG ATTCTTTACA GGATATAAAA GCATTGTTGg gtacccctgc agctgcgtcg
     3241 actctagagg atccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
     3301 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
     3361 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
     3421 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
     3481 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
     3541 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
     3601 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
     3661 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
     3721 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
     3781 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
     3841 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
     3901 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
     3961 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
     4021 tatgacctgc tgctggagat gctggacgcc caccgcctac atgcgcccac tagccgtgga
     4081 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
     4141 gagctccact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc
     4201 gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc
     4261 gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc
     4321 cttggaattg acgagtacgg tgggtaggga tccggttccg gaagtggatc cggatcctga
     4381 taccgtcgac ctcgagtcat gtaattagtt atgtcacgct tacattcacg ccctcccccc
     4441 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat
     4501 ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt
     4561 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
     4621 ggacgctcga aggctttaat ttgatgtcgt aataaccccg ccccgacgcc attttaagtc
     4681 caaaggcaca attttacgtt ggaactaaac ggtaaagatc aaatatcacc tttattcttt
     4741 tttggtatgc atcgatttct ttgccagtaa gaactacgtg caactctaca ttagatcaaa
     4801 atataacgga tcataaatgc aaacaacatt tgtccaaaag aaacaaaaat gttggcgttt
     4861 gtctcgaaca tgacacaata ctccttgatc gagggacatt gcccgattaa ctatgattta
     4921 tactttttac tacaggtact cgttctccct actatttcac cagtatacgg tcgttttatg
     4981 attcatttat tgcctataat ggtaaaaagg gacatcttaa ccttacgtgt tgcaatgaga
     5041 ccattctttt cgattcatca actgtgaaat ttctgaaatc cagtacaatg cgaaacgcta
     5101 ccgataatgg caccgtcttt tgaattatga ggcccagata tggcgttcaa tcttcaacaa
     5161 aaaaatgaat atcaaaacta aattctatct ggataacaat ctttctttgg tttgcagaaa
     5221 tatcattaaa aaacaatcca taactactaa ggaagggtat aaatggtttg gtttactaat
     5281 tttatatttt gatttgatta gcaattataa tctctaatat actatttctg tagccaaaaa
     5341 tctgcaatgt caataagtag caagtgccgc ttactcgtga gaatatcaac cttatagcat
     5401 atgttttcta tttttttttt ttgcttatgg agataatgaa cattgtacac atgaacaaag
     5461 tggtagttct ctctccttga ttttttgtgc ttgcgatata tataattgtt acattcaatg
     5521 gccatgctaa ataaaggttt tagctttgtt tcactcgtgc ccaggataat tgtttaaacc
     5581 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5641 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5701 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5761 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5821 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5881 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5941 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6001 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6061 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6121 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6181 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6241 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6301 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6361 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6421 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6481 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6541 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6601 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6661 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6721 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6781 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6841 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6901 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6961 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7021 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7081 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7141 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7201 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7261 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7321 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7381 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7441 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7501 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7561 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7621 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7681 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7741 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7801 tcgtc     
//
