* Block Type = 96-Well Block (0.2mL)
* Calibration Background is expired  = No
* Calibration Background performed on = 10-13-2010
* Calibration HRM MELTDOCTOR is expired = No
* Calibration HRM MELTDOCTOR performed on = 10-13-2010
* Calibration Pure Dye FAM is expired = No
* Calibration Pure Dye FAM performed on = 10-13-2010
* Calibration Pure Dye MELTDOCTOR is expired = No
* Calibration Pure Dye MELTDOCTOR performed on = 10-13-2010
* Calibration Pure Dye ROX is expired = No
* Calibration Pure Dye ROX performed on = 10-13-2010
* Calibration Pure Dye SYBR is expired = No
* Calibration Pure Dye SYBR performed on = 10-13-2010
* Calibration Pure Dye VIC is expired = No
* Calibration Pure Dye VIC performed on = 10-13-2010
* Calibration RNaseP is expired  = No
* Calibration RNaseP performed on = 10-15-2010
* Calibration ROI is expired  = No
* Calibration ROI performed on = 10-13-2010
* Calibration Uniformity is expired  = No
* Calibration Uniformity performed on = 10-13-2010
* Chemistry = TAQMAN
* Date Created = 2014-09-10 04:10:50 AM EDT
* Experiment Barcode = 
* Experiment Comment = NA
* Experiment File Name = C:\Program Files (x86)\Applied BioSystems\QuantStudio Real-Time PCR Software\examples\QS7Flex\QS7_96-Well Comparative Ct Example.eds
* Experiment Name = QuantStudio96-Well Comparative Ct Example
* Experiment Run End Time = 2010-10-20 02:23:34 AM EDT
* Experiment Type = Comparative Cт (ΔΔCт) 
* Instrument Type = QuantStudio(TM) 7 Flex System
* Instrument Name = 1
* Instrument Serial Number = 1
* Passive Reference = ROX
* Quantification Cycle Method = Ct
* Signal Smoothing On = true
* Stage/ Cycle where Analysis is performed = Stage 2, Step 2
* User Name = NA

[Sample Setup]
Well	Well Position	Sample Name	Sample Color	Biogroup Name	Biogroup Color	Target Name	Target Color	Task	Reporter	Quencher	Quantity	Comments
37	D1	Lung	"RGB(176,23,31)"			TGF-B	"RGB(176,23,31)"	UNKNOWN	FAM	NFQ-MGB		
38	D2	Lung	"RGB(176,23,31)"			TGF-B	"RGB(176,23,31)"	UNKNOWN	FAM	NFQ-MGB		
46	D10	Liver	"RGB(139,137,112)"			TGF-B	"RGB(176,23,31)"	UNKNOWN	FAM	NFQ-MGB		
49	E1	Lung	"RGB(176,23,31)"			TGF-B	"RGB(176,23,31)"	UNKNOWN	FAM	NFQ-MGB		
58	E10	Liver	"RGB(139,137,112)"			TGF-B	"RGB(176,23,31)"	UNKNOWN	FAM	NFQ-MGB		
85	H1	Lung	"RGB(176,23,31)"			GAPDH	"RGB(0,0,255)"	UNKNOWN	VIC	NFQ-MGB		
86	H2	Lung	"RGB(176,23,31)"			GAPDH	"RGB(0,0,255)"	UNKNOWN	VIC	NFQ-MGB		
94	H10	Liver	"RGB(139,137,112)"			GAPDH	"RGB(0,0,255)"	UNKNOWN	VIC	NFQ-MGB		
95	H11	Liver	"RGB(139,137,112)"			GAPDH	"RGB(0,0,255)"	UNKNOWN	VIC	NFQ-MGB		


[Amplification Data]
Well	Cycle	Target Name	Rn	Delta Rn
37	1	TGF-B	0.627	0.001
38	1	TGF-B	0.612	-0.001
46	1	TGF-B	0.607	0.000
49	1	TGF-B	0.620	0.001
58	1	TGF-B	0.614	0.001
85	1	GAPDH	1.165	0.003
86	1	GAPDH	1.220	0.002
94	1	GAPDH	1.231	0.003
95	1	GAPDH	1.217	0.002


[Results]
Well	Well Position	Omit	Sample Name	Target Name	Task	Reporter	Quencher	Quantity	Quantity Mean	Quantity SD	RQ	RQ Min	RQ Max	CT	Ct Mean	Ct SD	Delta Ct	Delta Ct Mean	Delta Ct SE	Delta Delta Ct	Automatic Ct Threshold	Ct Threshold	Automatic Baseline	Baseline Start	Baseline End	Comments	Amp Score	Cq Conf
37	D1	false	Lung	TGF-B	UNKNOWN	FAM	NFQ-MGB				0.800	0.639	1.002	30.155	30.115	0.051		-2.647	0.150	0.323	true	0.133	true	3	25		1.242	0.967
38	D2	false	Lung	TGF-B	UNKNOWN	FAM	NFQ-MGB				0.800	0.639	1.002	30.200	30.115	0.051		-2.647	0.150	0.323	true	0.133	true	3	25		1.242	0.964
46	D10	false	Liver	TGF-B	UNKNOWN	FAM	NFQ-MGB				1.000	0.789	1.267	29.799	29.799	0.031		-2.969	0.158	0.000	true	0.133	true	3	25		1.230	0.968
49	E1	false	Lung	TGF-B	UNKNOWN	FAM	NFQ-MGB				0.800	0.639	1.002	30.093	30.115	0.051		-2.647	0.150	0.323	true	0.133	true	3	25		1.247	0.965
58	E10	false	Liver	TGF-B	UNKNOWN	FAM	NFQ-MGB				1.000	0.789	1.267	29.773	29.799	0.031		-2.969	0.158	0.000	true	0.133	true	3	25		1.232	0.970
85	H1	false	Lung	GAPDH	UNKNOWN	VIC	NFQ-MGB							32.884	32.762	0.259					false	0.060	true	3	29		0.936	0.954
86	H2	false	Lung	GAPDH	UNKNOWN	VIC	NFQ-MGB							32.937	32.762	0.259					false	0.060	true	3	29		0.947	0.911
94	H10	false	Liver	GAPDH	UNKNOWN	VIC	NFQ-MGB							32.546	32.768	0.274					false	0.060	true	3	29		0.945	0.947
95	H11	false	Liver	GAPDH	UNKNOWN	VIC	NFQ-MGB							33.074	32.768	0.274					false	0.060	true	3	29		0.947	0.912

Analysis Type = Singleplex
Endogenous Control = GAPDH
RQ Min/Max Confidence Level = 95.0
Reference Sample = Liver

